1. Others
  2. Fluorescent Dye
  3. Calcein-AM

Calcein-AM  (Synonyms: Calcein acetoxymethyl ester)

製品番号: HY-D0041 純度: 97.34%
COA 取扱説明書

Calcein AM, has cell membrane permeability and can easily enter the cell. Calcein AM has no fluorescence and is hydrolyzed by endogenous esterase in the cell to produce polar molecule Calcein (Calcein), which has strong negative charge and cannot permeate the cell membrane. Calcein can emit strong green fluorescence, so it is often used with Propidium Iodide for cell viability/virulence detection, excitation/emission wavelength: 494/515 nm.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

Calcein-AM 構造式

Calcein-AM 構造式

CAS 番号 : 148504-34-1

容量 価格(税別) 在庫状況 数量
Solvent
100 μg(2 mg/mL * 50 μL in DMSO) USD 70 在庫あり
Solvent
500 μg(2 mg/mL * 250 μL in DMSO) USD 130 在庫あり
Synthetic products have potential research and development risk.

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 42 publication(s) in Google Scholar

Top Publications Citing Use of Products

顧客検証

IF

    Calcein-AM purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2022 Aug 11;21:20-31.  [Abstract]

    HUVECs proliferation is assessed by Calcein-AM, and the results show that HP-β-CD and HP-β-CD@Res could effectively promote the proliferation of HUVECs.

    Calcein-AM purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 Oct;9(30):e2203031.  [Abstract]

    XPS analysis of CaALN powder before and after immersing in FeCl3 solution for 24 h. Detection of intracellular Fe concentration using Calcein-AM in MGC 803 cells after treatment with NaALN, CaALN, and DFX at 25 mg/L for 24 h by FCM analysis. Then these cells are washed with PBS and incubated with Calcein-AM (0.5 μM) for 15 min at 37 °C in the dark.

    Calcein-AM purchased from MedChemExpress. Usage Cited in: Pharmaceuticals. 2022 Aug 26;15(9):1059.

    For U251 cells, Calcein-AM (10 μM) solution and 0.5 μL PI staining solution (15 μM) is added into each well, stained for 10 min.

    Calcein-AM purchased from MedChemExpress. Usage Cited in: Antioxidants (Basel). 2021 May 18;10(5):798.  [Abstract]

    HeLa cells are pretreated with 20 μM Fe(II) for 2 h and then incubated with Calcein-AM ( 0.5 μM; for 15 min at 37 °C). After 15 min, cells are washed and incubated with or without CAPE and its analogues for 15 min. The fluorescence intensity of cells is measured by flow cytometry.

    Calcein-AM purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2021 Jun;188:114583.  [Abstract]

    Cells are incubated with fluorescence probes Calcein-AM (1 μM) and propidium iodide (5 μM) for 5 min and imaged through fluorescence inverted microscope.

    Calcein-AM purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2020 Sep;10(9):1694-1708.  [Abstract]

    HUVECs are pre-treated with SMI or Rd for 24 h and seeded on Matrigel for 5.5 h followed by 0.5 h of Calcein-AM staining.

    Calcein-AM purchased from MedChemExpress. Usage Cited in: EMBO Rep. 2019 Jul;20(7):e47563.  [Abstract]

    Cells are trypsinized, washed twice with 0.5 ml PBS, and then incubated with 0.2 μM Calcein‐AM at 37°C for 15 min. Then, the cells were washed twice with 0.5 ml PBS and either incubated with 100 μM Deferiprone or left untreated at 37°C for 1 h. The cells are analyzed with a flow cytometer. Calcein-AM is excited at 488 nm, and fluorescence is measured at 525 nm.
    • 生物活性

    • プロトコル

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Calcein AM, has cell membrane permeability and can easily enter the cell. Calcein AM has no fluorescence and is hydrolyzed by endogenous esterase in the cell to produce polar molecule Calcein (Calcein), which has strong negative charge and cannot permeate the cell membrane. Calcein can emit strong green fluorescence, so it is often used with Propidium Iodide for cell viability/virulence detection, excitation/emission wavelength: 494/515 nm[1].

    体外実験

    General Protocol

    1.Preparation of Calcein AM working solution
    Preparation of 5 mM stock solution with DMSO.
    Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
    Note: Please adjust the concentration of Calcein AM working solution according to the actual situation.

    2.Cell staining
    2.1 Suspension cells(6-well plate)
    a.Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
    b.Add 1 mL of working solution, and then incubate at room temperature for 30-45 minutes.
    c.Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
    d.Wash twice with PBS, 5 minutes each time.
    e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or fluorescence microplate readers.
    2.2 Adherent cells
    a.Culture adherent cells on sterile coverslips.
    b.Remove the coverslip from the medium and aspirate excess medium.
    c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 30-45 minutes.
    d.Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or fluorescence microplate readers.

    Storage
    -20°C
    Protect from light

    Precautions
    1.Please adjust the concentration of Calcein AM working solution according to the actual situation.
    2.This product is for R&D use only, not for drug, household, or other uses.
    3.For your safety and health, please wear a lab coat and disposable gloves to operate.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    体内実験

    Calcein-AM is found to be suitable for in vivo studies, because it has no deleterious effects on cell function and is, indeed, a marker of cell viability[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    分子量

    994.86

    分子式

    C46H46N2O23

    Emission (Em)

    515

    Excitation (Ex)

    485

    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C1OC2(C(C=C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OC(C)=O)=C3)=C3OC4=CC(OC(C)=O)=C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C24)C5=C1C=CC=C5

    輸送条件

    Room temperature in continental US; may vary elsewhere.

    保管条件

    -20°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (100.52 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.0052 mL 5.0258 mL 10.0517 mL
    5 mM 0.2010 mL 1.0052 mL 2.0103 mL
    10 mM 0.1005 mL 0.5026 mL 1.0052 mL
    *Please refer to the solubility information to select the appropriate solvent.
    純度とドキュメンテーション

    純度: ≥98.0%

    Dyeing Example
    参考文献
    細胞実験
    [1][2][3]

    K562, Daudi, and Chang liver cells are labeled with calcein-AM. Calcein-AM's excitation and emission wavelengths are 496 nm and 520 nm, respectively. The filter/mirror combination used to detect calcein-AM's green fluorescence includes the 490-nm excitation and 520-nm emission filters with a dichroic mirror. Differences in the automatic fluorescence readings between the test and control wells determine the results[1]. A simple and sensitive cell-cell adhesion microplate assay is established using the calcein-AM. The procedure involves three steps: the labeling of lymphocytes with an adequate concentration of calcein-AM (20 μM) during a short incubation period (30 min); the adhesion of 2×105 labeled lymphocytes per well to confluent keratinocyte or fibroblast monolayers grown in microtiter plates for 90 min; and, finally, measurement of the fluorescent signal utilizing a new system of cold-light microfluorimetry[2]. Cells are incubated for 15 min in 1 mL of a 1% saponin solution in PBS buffer, pH 7.4, containing 0.05% sodium azide. After saponin permeabilization, 4×105 RBCs in suspension in PBS buffer containing 0.1% saponin and 0.05% sodium azide are incubated (37°C in the dark for 45 min) with calcein-AM to a final concentration of 5 μM, ished three times with the same PBS buffer containing 0.1% saponin and 0.05% sodium azide, and the cell viability is analyzed by flow cytometry[3].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。

    参考文献
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Calcein-AM Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    • モル濃度カルキュレーター

    • 希釈カルキュレーター

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    最近チェックした製品:

    オンラインお問い合わせ

    Your information is safe with us. * Required Fields.

    製品名

     

    タイトル

    お名前 *

     

    PC 用メールアドレス *

    電話番号 *

     

    勤務先/学校名 *

    Department *

     

    カスタマ需要量 *

    国会或いは地域 *

         

    必ず会社名を記載ください。個人への返信は行いません。

    メッセージ

    バルクお問い合わせ

    Inquiry Information

    製品名:
    Calcein-AM
    製品番号:
    HY-D0041
    数量:
    MCE 日本正規代理店: