Glyoxalase I inhibitor
Based on 4 publication(s) in Google Scholar
Glyoxalase I inhibitor (3(Et)2) is the inhibitor for glyoxalase I (GLO), and can be used in antitumor research.
For research use only. We do not sell to patients.
- CAS No.: 221174-33-0
- Formula: C21H30BrClN4O8S
- Molecular Weight:613.91
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Glyoxalase I inhibitor
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Biological Activity
Glyoxalase I inhibitor (100 μM, 48 h) inhibits glyoxalase I, causes accumulation of methylglyoxal and leads to cytotoxicity in cells L1210 and B16 with GI50 of 3 μM and 11 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:L1210 and B16
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Concentration:100 μM
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Incubation Time:48 h
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Result:Inhibited cell growth.
Chemical Information
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CAS No. 221174-33-0
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Appearance Solid
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Molecular Weight 613.91
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Formula C21H30BrClN4O8S
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Color Off-white to light yellow
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SMILES
BrC1=CC=C(N(O)C(SC[C@H](NC(CC[C@H](N)C(OCC)=O)=O)C(NCC(OCC)=O)=O)=O)C=C1.[H]Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (4)
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Journal Impact Factor
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Most Recent
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Nature
A metabolite-derived protein modification integrates glycolysis with KEAP1-NRF2 signalling. [Abstract]2018 Oct;562(7728):600-604. PMID: 30323285 -
Cell
2024 Jan 18;187(2):294-311.e21. PMID: 38128537 -
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Solvent & Solubility
DMSO : ≥ 37 mg/mL (60.27 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.07 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.07 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6289 mL | 8.1445 mL | 16.2890 mL | 40.7226 mL |
| 5 mM | 0.3258 mL | 1.6289 mL | 3.2578 mL | 8.1445 mL | |
| 10 mM | 0.1629 mL | 0.8145 mL | 1.6289 mL | 4.0723 mL | |
| 15 mM | 0.1086 mL | 0.5430 mL | 1.0859 mL | 2.7148 mL | |
| 20 mM | 0.0814 mL | 0.4072 mL | 0.8145 mL | 2.0361 mL | |
| 25 mM | 0.0652 mL | 0.3258 mL | 0.6516 mL | 1.6289 mL | |
| 30 mM | 0.0543 mL | 0.2715 mL | 0.5430 mL | 1.3574 mL | |
| 40 mM | 0.0407 mL | 0.2036 mL | 0.4072 mL | 1.0181 mL | |
| 50 mM | 0.0326 mL | 0.1629 mL | 0.3258 mL | 0.8145 mL | |
| 60 mM | 0.0271 mL | 0.1357 mL | 0.2715 mL | 0.6787 mL |
Glyoxalase I inhibitor Related Classifications
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