Saikosaponin D

Name: Saikosaponin D
Brand: MedChemExpress
SKU: HY-N0250
Rating: 5.0 (10 reviews)

Cat. No.: HY-N0250 Purity: 99.81%: Data Sheet
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Saikosaponin D is a triterpene saponin isolated from Bupleurum, with anti-inflammatory, anti-bacterial, anti-tumor, and anti-allergic activities; Saikosaponin D inhibits selectin, STAT3 and NF-kB and activates estrogen receptor-β.

For research use only. We do not sell to patients.

CAS No. : 20874-52-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 10 publication(s) in Google Scholar

Other Forms of Saikosaponin D:

Saikosaponin D (Standard) Get quote

10 Publications Citing Use of MCE Saikosaponin D

Cell Proliferation/Viability Assay

Cell Imaging/Staining

RT-PCR

Histological Imaging/Staining

: Saikosaponin D purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2021 Nov 23;15:4741-4757. [Abstract]; Cell viability of BMMs (bone marrow-derived macrophages) was evaluated by CCK-8 at 48, 96 h treated with Saikosaponin D (0.5, 1, 2, 4, 8, 16 μM).

: Saikosaponin D purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2021 Nov 23;15:4741-4757. [Abstract]; The role of Saikosaponin D (SSD) (0.5, 1 μM) in osteoclast formation in a dose-dependent manner. BMMs stimulated by RANKL (50 ng/mL) were treated with or without various concentrations of SSD for 7 days. After fixing, the cells subjected to TRAP staining.

: Saikosaponin D purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2021 Nov 23;15:4741-4757. [Abstract]; mRNA expression profile of OMG (osteoclast marker genes) (time-dependent). RT-qPCR (real-time quantitative PCR) was performed on total RNA extracted from cells stimulated by RANKL (50 ng/mL) and treated with 2 μM Saikosaponin D (SSD) for the indicated time points during the 5 day osteoclast culture.

: Saikosaponin D purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2021 Nov 23;15:4741-4757. [Abstract]; BMMs were treated without or with various concentrations of SSD (0.5, 1 and 2 μmol/L) for 1 h and then were treated with RANKL (50 ng/mL) for 15 min. Total proteins extracted from BMMs were probed for protein levels using immunoblot analyses.

: Saikosaponin D purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2021 Nov 23;15:4741-4757. [Abstract]; H&E staining and TRAP staining of calvaria from the high-dose Saikosaponin D (SSD) (4 mg/kg) group, low-dose SSD (2 mg/kg) group, LPS group, and sham group.

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[2]^[3]

STAT3

NF-κB

ERβ

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	30.82 μM Compound: 9	Cytotoxicity against human A549 cells assessed as induction of cell death incubated for 2 days by MTT assay Cytotoxicity against human A549 cells assessed as induction of cell death incubated for 2 days by MTT assay	[PMID: 26259802]
Bcap37	IC₅₀	10.67 μM Compound: 9	Cytotoxicity against human Bcap37 cells assessed as induction of cell death incubated for 2 days by MTT assay Cytotoxicity against human Bcap37 cells assessed as induction of cell death incubated for 2 days by MTT assay	[PMID: 26259802]
HeLa	IC₅₀	10 μM Compound: 26	Antiproliferative activity against human HeLa cells Antiproliferative activity against human HeLa cells	[PMID: 32030976]
Hep 3B2	IC₅₀	9.61 μM Compound: 9	Cytotoxicity against human Hep3B cells assessed as induction of cell death incubated for 2 days by MTT assay Cytotoxicity against human Hep3B cells assessed as induction of cell death incubated for 2 days by MTT assay	[PMID: 26259802]
HepG2	IC₅₀	4.88 μM Compound: 9	Cytotoxicity against human HepG2 cells assessed as induction of cell death incubated for 2 days by MTT assay Cytotoxicity against human HepG2 cells assessed as induction of cell death incubated for 2 days by MTT assay	[PMID: 26259802]
MCF7	IC₅₀	7.68 μM Compound: 9	Cytotoxicity against human MCF7 cells assessed as induction of cell death incubated for 2 days by MTT assay Cytotoxicity against human MCF7 cells assessed as induction of cell death incubated for 2 days by MTT assay	[PMID: 26259802]

In Vitro

Saikosaponin D (Compound 3) is a triterpene saponin, which inhibits E-selectin, L-selectin and P-selectin binding to THP-1 cells, with IC₅₀s of 1.8 μM, 3.0 μM and 4.3 μM, and such effects are not due to cytotoxic action. Saikosaponin D (1, 5, 10 μM) dose-dependently inhibits the THP-1 adhesion to the HUVECs monolayer activated by TNF-α. Saikosaponin D (30 μM) also inhibits the expression of P-selectin ligand (CD162) in THP-1 cells^[1]. Saikosaponin D (5 μM) suppresses the proliferation of HSC-T6 cells induced by H₂O₂ treatment, reduces the expression levels of α-SMA, TGF-β1, Hyp, COL1 and TIMP-1, and increases MMP-1 expressioon, thus inhibiting H₂O₂-induced excessive extracellular matrix (ECM) formation, with similar effects to estradiol (E2), and these effects are blocked by ER antagonists. Saikosaponin D also inhibits oxidative stress-induced ROS generation and down reduates MAPK signaling pathway, and the inhibition is also suppressed by ER antagonists^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Saikosaponin D (2 mg/kg/day, i.p.) shows a protective effect on overdose of acetaminophen (APAP)-induced liver injury of mice. Saikosaponin D affects APAP metabolism, increases GSH levels but does not alter PPARα activation. Saikosaponin D (2 mg/kg/day, i.p.) also suppresses APAP-induced increases in the expression of STAT3 target genes and pro-inflammatory cytokines and inhibits APAP-induced activation of STAT3 and NF-kB^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

780.98

Formula

C₄₂H₆₈O₁₃

CAS No.

20874-52-6

Appearance

Solid

Color

White to off-white

SMILES

CC1(C)CC[C@]2(CO3)[C@H](O)C[C@@]4(C)[C@]([C@]5([H])C=C[C@]43[C@]2([H])C1)(C)CC[C@@]([C@]5(C)CC6)([H])[C@@](CO)(C)[C@H]6O[C@@](O[C@H](C)[C@@H]7O)([H])[C@H](O)[C@H]7O[C@@]8([H])[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O8

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (64.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2804 mL	6.4022 mL	12.8044 mL
5 mM	0.2561 mL	1.2804 mL	2.5609 mL
10 mM	0.1280 mL	0.6402 mL	1.2804 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.81%

Select Batch:

Data Sheet (283 KB) SDS (393 KB)

COA (253 KB) HNMR (372 KB) RP-HPLC (208 KB)

Handling Instructions (2659 KB)

References

[1]. Jang MJ, et al. Saikosaponin D isolated from Bupleurum falcatum inhibits selectin-mediated cell adhesion. Molecules. 2014 Dec 4;19(12):20340-9. [Content Brief]

[2]. Liu A, et al. Saikosaponin d protects against acetaminophen-induced hepatotoxicity by inhibiting NF-κB and STAT3 signaling. Chem Biol Interact. 2014 Nov 5;223:80-6. [Content Brief]

[3]. Que R, et al. Estrogen receptor-β-dependent effects of saikosaponin‑d on the suppression of oxidative stress-induced rat hepatic stellate cell activation. Int J Mol Med. 2018 Mar;41(3):1357-1364. [Content Brief]

Cell Assay ^[1]	Cell viability is assessed by morphology and by reduction of the tetrazolium salt (MTT). Briefly, the THP-1 cells (2 × 10⁵ cells/well) and various concentrations of compounds 1-4 (including Saikosaponin D) are added to the 96-well plates, incubated for 48 h at 37°C, and 5 µL of MTT solution (5 mg/mL in PBS) is added to each well of the 96-well plates. After incubation for 4 h at 37°C, the absorbance is measured at 540 nm using a microplate reader with the reference absorbance at 650 nm^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[2]	Mice^[2] Male 6- to 7-week-old C57BL6 mice are randomly divided into four groups, vehicle/control, Saikosaponin D (SSd)/control, vehicle/APAP, and SSd/APAP, and killed 4 h or 24 h after single APAP injection. For overdose of acetaminophen (APAP) injection, a typical single dose of 200 mg/kg/day is used. Saikosaponin D, 2 mg/kg once daily is used as the dosing regimen. Saikosaponin D powder is dissolved in a saline solution supplemented with 0.1% Tween 20 and is administered by intraperitoneal injection at a dose of 2 mg/kg/day once daily for five days. Saline solution containing 0.1% Tween 20 without Saikosaponin D is administered as a vehicle. APAP is dissolved in warm saline solution (20 mg/mL) and is injected intraperitoneally 30 minutes after the last Saikosaponin D injection. Saline is injected to mice in the control groups^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Jang MJ, et al. Saikosaponin D isolated from Bupleurum falcatum inhibits selectin-mediated cell adhesion. Molecules. 2014 Dec 4;19(12):20340-9. [Content Brief] [2]. Liu A, et al. Saikosaponin d protects against acetaminophen-induced hepatotoxicity by inhibiting NF-κB and STAT3 signaling. Chem Biol Interact. 2014 Nov 5;223:80-6. [Content Brief] [3]. Que R, et al. Estrogen receptor-β-dependent effects of saikosaponin‑d on the suppression of oxidative stress-induced rat hepatic stellate cell activation. Int J Mol Med. 2018 Mar;41(3):1357-1364. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.2804 mL	6.4022 mL	12.8044 mL	32.0111 mL
	5 mM	0.2561 mL	1.2804 mL	2.5609 mL	6.4022 mL
	10 mM	0.1280 mL	0.6402 mL	1.2804 mL	3.2011 mL
	15 mM	0.0854 mL	0.4268 mL	0.8536 mL	2.1341 mL
	20 mM	0.0640 mL	0.3201 mL	0.6402 mL	1.6006 mL
	25 mM	0.0512 mL	0.2561 mL	0.5122 mL	1.2804 mL
	30 mM	0.0427 mL	0.2134 mL	0.4268 mL	1.0670 mL
	40 mM	0.0320 mL	0.1601 mL	0.3201 mL	0.8003 mL
	50 mM	0.0256 mL	0.1280 mL	0.2561 mL	0.6402 mL
	60 mM	0.0213 mL	0.1067 mL	0.2134 mL	0.5335 mL

Saikosaponin D Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Saikosaponin D20874-52-6STATNF-κBEstrogen Receptor/ERRBacterialNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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Saikosaponin D

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Saikosaponin D Related Antibodies

10 Publications Citing Use of MCE Saikosaponin D

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References

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Complete Stock Solution Preparation Table

Saikosaponin D Related Classifications

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