eRF3b/GSPT2

Eukaryotic release factor 3b (eRF3b), also known as G1 to S phase transition protein 2 (GSPT2), is a member of the eRF3 family of translation termination factors that participates in stop codon recognition and polypeptide release through interaction with eukaryotic release factor 1 (eRF1)^[1][2]. GSPT2 belongs to the GTPase family of release factors and contains conserved regions involved in eRF1 binding and translation termination complex formation^[1][3]. Mechanistically, eRF3 proteins promote translation termination by facilitating GTP-dependent conformational changes required for efficient peptide release^[2]GSPT2 expression and function have been studied in translation regulation models because eRF3 family proteins contribute to maintaining accurate protein synthesis termination^[2][4]. Compared with the related isoform eRF3a/GSPT1, GSPT2 shows a more restricted expression pattern and shares conserved eRF1-interacting domains required for translation termination activity^[3][5]. Unlike eRF3a/GSPT1, which is broadly expressed and frequently studied in cancer-associated translation regulation, GSPT2 has been investigated primarily as a translation termination factor with distinct expression characteristics^[5]. For experimental applications, GSPT2 function is analyzed using protein interaction studies, translation termination assays, expression profiling, and genetic manipulation approaches^[3][4]. The limited availability of GSPT2-selective modulators has resulted in greater reliance on molecular and cellular approaches to study its role in translation termination^[4].