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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D2883C
    RB-PEG2000-SH
    RB-PEG2000-SH (Rhodamine B-PEG2000-SH) is a fluorescent dye composed of Rhodamine B (HY-Y0016), PEG and sulfhydryl (SH). SH is highly reactive and can react with a variety of functional groups (such as maleimide) to form stable thioether bonds (Ex/Em=546/610 nm).
    RB-PEG2000-SH
  • HY-D2183
    6-Carboxy-JF5252
    6-Carboxy-JF5252 is a fluorophore. 6-Carboxy-JF5252 can be used as probes, dyes, tags. 6-Carboxy-JF5252 can be used for the research of super-resolution imaging.
    6-Carboxy-JF5252
  • HY-DY1088
    Fluorescein-5-maleimide (solution)
    Fluorescein-5-maleimide (solution) (N-(5-Fluoresceinyl)maleimide (solution)) is a fluorescent dye. Fluorescein-5-maleimide can be used to detect the redox state of thiols in eukaryotic cells. Fluorescein-5-maleimide can label peptides and is used to detect negatively charged nanoparticles. Fluorescein-5-maleimide can also label actin to explore its interaction with cardiac myosin-binding protein C (cMyBP-C), which helps in developing small molecule modulators for heart failure. Fluorescein-5-maleimide can screen mutant proteins that contain cysteine residues. The excitation wavelength of Fluorescein-5-maleimide is 494 nm, and the emission wavelength is 519 nm.

    Solvent and concentration: DMSO: 10 mM
    Fluorescein-5-maleimide (solution)
  • HY-NP0189
    Succinylated Wheat Germ Agglutinin (Biotinylated)
    Succinylated Wheat Germ Agglutinin (WGA) Biotinylated is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Succinylated Wheat Germ Agglutinin (WGA) Biotinylated is a biological material or organic compound that can be used in life science research.
    Succinylated Wheat Germ Agglutinin (Biotinylated)
  • HY-D2401F1
    Cy5-Polystyrene microsphere (20um)
    Cy5-Polystyrene microsphere (20um) is a Cy5 (HY-D0821)-labeled microsphere that can be used for bioimaging and drug delivery.
    Cy5-Polystyrene microsphere (20um)
  • HY-23926
    PYBG
    		99.87%
    PYBG acts as a versatile precursor to be facilely conjugated with various fluorescent dyes through ‘Click chemistry’ and Sonogashira coupling reactions. PYBG is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
    PYBG
  • HY-D1883
    Cy7.5-COOH
    Cy7.5-COOH (compound 8f), a cyanine dye, is a highly fluorescent compound (Abs/Em = 781/808 nm). Cy7.5-COOH can be used as a fluorescent probe in a wide-range of applications such as DNA sequencing, flow cytometry and in vivo imaging.
    Cy7.5-COOH
  • HY-D1514
    NIR-641 N-succinimidyl ester
    NIR-641 N-succinimidyl ester is a fluorescent dye.
    NIR-641 N-succinimidyl ester
  • HY-138760
    SEluc-2
    SEluc-2 is a small-molecule probe based on the firefly luciferin. SEluc-2, a bioluminescent probe for the sensitive and selective detection of thiols in living cells.
    SEluc-2
  • HY-D1575
    C6 NBD Galactosylceramide
    C6 NBD galactosylceramide is an active derivative of galactosylceramide that is tagged with fluorescent C6 nitrobenzoxadiazole (C6 NBD). C6 NBD galactosylceramide can be used as a substrate for neutral β-glycosylceramidase (GCase) to study intracellular localization and metabolism of galactosylceramide (Ex=nm, Em=525).
    C6 NBD Galactosylceramide
  • HY-N8502
    Urdamycin A
    Urdamycin A (Compound 3b) is an angucycline antibiotic that can be isolated from Streptomyces fradiae. Urdamycin A is an orange indicator with a change of the color to ultramarine blue at pH 7.7. Urdamycin A has anticancer activity with IC50s of 2.4 and 0.55 μg/mL in proliferation and stem cell assays, respectively.
    Urdamycin A
  • HY-D2840H
    FITC-mPEG10000
    FITC-mPEG10000 is a fluorescent dye composed of methoxypolyethylene glycol (mPEG) and FITC (HY-66019). FITC-mPEG10000 can be used for fluorescent labeling and imaging.
    FITC-mPEG10000
  • HY-D2171
    AF488 DBCO
    AF488 DBCO is a fluorescent dye and also a click chemistry reagent that can label azide-containing biomolecules. AF488 DBCO mediates the coupling reaction with azide-modified 2'-OMe RNA oligonucleotides. DBCO is a bioorthogonal partner of azides and enables covalent coupling without copper (Ex/Em = 470/520 nm).
    AF488 DBCO
  • HY-D1568A
    Sulfo-Cy7.5 NHS ester tripotassium
    Sulfo-Cy7.5 NHS ester tripotassium is a near infrared water soluble hydrophilic dye, also is an NHS ester for the modification of amine groups. Sulfo-Cy7.5 NHS ester tripotassium contains a trimeth ylene bridge and has a linker arm for its attachment to proteins, peptides, and other molecules. Sulfo-Cy7.5 NHS ester tripotassium can be used for the research of NIR imaging applications.
    Sulfo-Cy7.5 NHS ester tripotassium
  • HY-D0151
    Tetramethylrhodamine-5-maleimide
    Tetramethylrhodamine-5-maleimide is a fluorescent probe with high specificity for cysteine thiol (SH) groups. Tetramethylrhodamine-5-maleimide has high sensitivity, responds rapidly to negatively charged nanoparticles, and shows a concentration-dependent decrease in fluorescence intensity, but it is susceptible to interference from humic acids.
    Tetramethylrhodamine-5-maleimide
  • HY-P4323
    Boc-Gln-Arg-Arg-AMC
    Boc-Gln-Arg-Arg-AMC is a fluorogenic substrate for the determination of protease activity. Boc-Gln-Arg-Arg-AMC undergoes hydrolysis and releases the fluorescent product 7-amino-4-methylcoumarin (AMC). AMC is fluorescent under UV light and can emit a fluorescent signal.
    Boc-Gln-Arg-Arg-AMC
  • HY-D2844B
    FITC-PEG2000-FA
    FITC-PEG2000-FA is a fluorescent dye composed of Folic acid (HY-16637) (FA), polyethylene glycol (PEG) and FITC (HY-66019). Fluorescent dye is linked to PEG and Folic acid through a specific chemical reaction to form a molecule with special properties. FITC-PEG2000-FA can be used for fluorescent labeling and imaging.
    FITC-PEG2000-FA
  • HY-N16306
    Lyso-laurdan
    Lyso-laurdan, a derivative of Laurdan (HY-D0080), is a fluorescent probe. Lyso-Laurdan accumulates in the lysosome via a morpholine group that is entrapped in lysosome via protonation to its cationic form in the acidic compartment. Lyso-laurdan is localized to lysosomes, allowing for the study of lysosomal membranes.
    Lyso-laurdan
  • HY-DY1087
    Coppersensor 1 (solution)
    Coppersensor 1 (solution) is a membrane-permeable fluorescent dye. Coppersensor-1 has a picomolar affinity for Cu+ with high selectivity over competing cellular metalions. Coppersensor-1 as a probe, can selective and sensitive detection of copper(I) ions (Cu+) in biological samples, including live cells. Coppersensor-1 can be used for the research of imaging of severe diseases such as cancer, cardiovascular disorders and neurogenerative diseases.
    Solvent and concentration: DMSO: 2 mM
    Coppersensor 1 (solution)
  • HY-134145
    1-(Anilinocarbonyl)proline
    		99.60%
    1-(Anilinocarbonyl)proline can be used to identify dual action probes in a cell model of Huntington.
    1-(Anilinocarbonyl)proline
Cat. No. Product Name / Synonyms Application Reactivity