1. Biochemical Assay Reagents
  2. Tetrazolium Red

Tetrazolium Red (Synonyms: 2,3,5-Triphenyltetrazolium chloride; TPTZ; TTC)

Cat. No.: HY-D0714 Purity: 99.66%
Handling Instructions

Tetrazolium Red(2,3,5-Triphenyltetrazolium chloride; TPTZ) is used to visualize dehydrogenase enzyme activity; initially the tetrazolium solution is colorless but changes to red when it comes into contact with hydrogen.

For research use only. We do not sell to patients.

Tetrazolium Red Chemical Structure

Tetrazolium Red Chemical Structure

CAS No. : 298-96-4

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Estimated Time of Arrival: December 31
5 g USD 60 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

Tetrazolium Red(2,3,5-Triphenyltetrazolium chloride; TPTZ) is used to visualize dehydrogenase enzyme activity; initially the tetrazolium solution is colorless but changes to red when it comes into contact with hydrogen. Tetrazolium red is used in a biochemical viability test for seeds. The test relies on dehydrogenase enzymes to release hydrogen ions which subsequently reduce the colorless tetrazolium salt solution to a red compound called formazan. Living cells turn red while dead cells remain colorless.

Molecular Weight

334.80

Formula

C₁₉H₁₅ClN₄

CAS No.

298-96-4

SMILES

C1(C2=CC=CC=C2)=NN(C3=CC=CC=C3)[N+](C4=CC=CC=C4)=N1.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 150 mg/mL (448.03 mM; Need ultrasonic)

H2O : 50 mg/mL (149.34 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9869 mL 14.9343 mL 29.8686 mL
5 mM 0.5974 mL 2.9869 mL 5.9737 mL
10 mM 0.2987 mL 1.4934 mL 2.9869 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (7.47 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (7.47 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (7.47 mM); Clear solution

*All of the co-solvents are provided by MCE.

Purity: 99.66%

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Keywords:

Tetrazolium Red2,3,5-TriphenyltetrazoliumTPTZTTCOthersInhibitorinhibitorinhibit

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Product name:
Tetrazolium Red
Cat. No.:
HY-D0714
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