Tetrazolium Red (Synonyms: 2,3,5-Triphenyltetrazolium chloride; TPTZ; TTC)

Name: Tetrazolium Red
Brand: MedChemExpress
SKU: HY-D0714
Rating: 4.5 (2 reviews)

Cat. No.: HY-D0714 Purity: 99.85%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Tetrazolium Red (2,3,5-Triphenyltetrazolium chloride; TTC) is used to observe the activity of dehydrogenase, and it turns colorless to red when exposed to hydrogen. Tetrazolium Red is a colorless, water-soluble dye that is reduced to a deep red, water-insoluble compound (formazan) mainly in the mitochondria of living cells, thereby distinguishing between surviving and infarcted brain tissue after stroke.

For research use only. We do not sell to patients.

Tetrazolium Red Chemical Structure

CAS No. : 298-96-4

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
5 g	USD 60	In-stock	Estimated Time of Arrival: December 31
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This product is a controlled substance and not for sale in your territory.

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Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE Tetrazolium Red

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Description

Molecular Weight

334.80

Formula

C₁₉H₁₅ClN₄

CAS No.

298-96-4

Appearance

Solid

Color

White to light yellow

SMILES

C1(C2=CC=CC=C2)=NN(C3=CC=CC=C3)[N+](C4=CC=CC=C4)=N1.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (149.34 mM; Need ultrasonic)

DMSO : 16.67 mg/mL (49.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9869 mL	14.9343 mL	29.8686 mL
5 mM	0.5974 mL	2.9869 mL	5.9737 mL
10 mM	0.2987 mL	1.4934 mL	2.9869 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.67 mg/mL (4.99 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.67 mg/mL (4.99 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.67 mg/mL (4.99 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 25 mg/mL (74.67 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.85%

Select Batch:

Data Sheet (279 KB) SDS (544 KB)

COA (258 KB) LCMS (264 KB)

Handling Instructions (2659 KB)

References

[1]. Sun YY, et al. Mannitol-facilitated perfusion staining with 2,3,5-triphenyltetrazolium chloride (TTC) for detection of experimental cerebral infarction and biochemical analysis. J Neurosci Methods. 2012 Jan 15;203(1):122-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	2.9869 mL	14.9343 mL	29.8686 mL	74.6714 mL
	5 mM	0.5974 mL	2.9869 mL	5.9737 mL	14.9343 mL
	10 mM	0.2987 mL	1.4934 mL	2.9869 mL	7.4671 mL
	15 mM	0.1991 mL	0.9956 mL	1.9912 mL	4.9781 mL
	20 mM	0.1493 mL	0.7467 mL	1.4934 mL	3.7336 mL
	25 mM	0.1195 mL	0.5974 mL	1.1947 mL	2.9869 mL
	30 mM	0.0996 mL	0.4978 mL	0.9956 mL	2.4890 mL
	40 mM	0.0747 mL	0.3734 mL	0.7467 mL	1.8668 mL
H₂O	50 mM	0.0597 mL	0.2987 mL	0.5974 mL	1.4934 mL
	60 mM	0.0498 mL	0.2489 mL	0.4978 mL	1.2445 mL
	80 mM	0.0373 mL	0.1867 mL	0.3734 mL	0.9334 mL
	100 mM	0.0299 mL	0.1493 mL	0.2987 mL	0.7467 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Tetrazolium Red Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tetrazolium Red298-96-42,3,5-Triphenyltetrazolium TPTZ TTCBiochemical Assay Reagentsdehydrogenasewater-solubleinfarctedstrokebrain tissueInhibitorinhibitorinhibit

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Tetrazolium Red (Synonyms: 2,3,5-Triphenyltetrazolium chloride; TPTZ; TTC)

Customer Review

Tetrazolium Red Related Antibodies

2 Publications Citing Use of MCE Tetrazolium Red

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Tetrazolium Red Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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