Evans Blue (Synonyms: Direct Blue 53; T-1824; C.I. 23860)

Name: Evans Blue
Brand: MedChemExpress
SKU: HY-B1102
Rating: 5.0 (29 reviews)

Cat. No.: HY-B1102 Purity: 99.86%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Evans Blue (Direct Blue 53) is a potent inhibitor of L-glutamate uptake via the membrane bound excitatory amino acid transporter (EAAT). Evans Blue is a L-glutamate and kainate receptor-mediated currents inhibitor. Evans Blue has a strong affinity towards serum albumin, making it a high molecular weight protein tracer. Evans Blue is also used to study BBB (blood-brain barrier) permeability.

For research use only. We do not sell to patients.

CAS No. : 314-13-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote
5 g	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 29 publication(s) in Google Scholar

Other Forms of Evans Blue:

Neutrophil elastase-IN-7 Get quote

29 Publications Citing Use of MCE Evans Blue

In Vivo Efficacy Study

: Evans Blue purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Oct 4;330(Pt 3):148106. [Abstract]; Representative images of injured spinal cord in the control, ZFgf11a, and MFGF11b groups after Evans Blue (EB, 2 mL/kg, i.v.; single dose) dye injection at 4 dpi.

: Evans Blue purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Oct 4;330(Pt 3):148106. [Abstract]; Representative immunofluorescence images of Evans Blue (EB, 2 mL/kg, i.v.; single dose) extravasation at the lesion epicenter in the transverse sections of the spinal cord in the control, ZFgf11a, and MFGF11b groups at 4 dpi.

: Evans Blue purchased from MedChemExpress. Usage Cited in: Cancer Immunol Immunother. 2025 Jul 12;74(8):268. [Abstract]; Representative images of Evans blue (4 ml/kg; i.v.; single dose) leakage and the difference between TGF-β3 and PBS groups (n = 5).

: Evans Blue purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2024 Jan;13(3):e2302320. [Abstract]; The effects of different groups (control, CLP, Ca-MOF, Zolif, CMZ, RCMZ, and γ3-RCMZ) on CLP-induced vascular permeability were examined by measuring Evans blue dye in peritoneal washings. Evans blue (10 mg/kg; i.v.; single dose) injection revealed that γ3-RCMZ effectively reduced endothelial leakage levels to one-sixth of those observed in the CLP group, indicating its predominant antiendothelial leakage effect in sepsis.

: Evans Blue purchased from MedChemExpress. Usage Cited in: Theranostics. 2023 Jun 26;13(11):3761-3780. [Abstract]; Sufu-cKO and WT mice were intraperitoneally injected with PBS or LPS (5 mg/kg) for 23 h, then injected with Evans blue dye (30 mg/kg body weight; i.v.; single dose) for an additional 40 min before mice were killed and lung tissues harvested. Means ± SEM (n = 5).

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AMPA Receptor NMDA Receptor Kainate Receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
Sf9	IC₅₀	0.922 μM Compound: 20, NSC-8680	Inhibition of human recombinant C-terminal FLAG-tagged autotaxin expressed in baculovirus-infected Sf9 cells assessed as FS-3 hydrolysis Inhibition of human recombinant C-terminal FLAG-tagged autotaxin expressed in baculovirus-infected Sf9 cells assessed as FS-3 hydrolysis	[PMID: 20349977]

In Vitro

Evans blue, a bis-azo dye, is a non-permeating dye. In presence of plasma membrane damage, Evans blue enters in the cytoplasm and nucleus, thereby staining them blue. Evans blue is used for checking cell viability^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The amount of Evans Blue (2% solution; 4 mL/kg) stain accumulated in the brains of mice after ICH produced by collagenase injection was independent of the stain administration route^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	CD-1 mice bearing intracerebral hemorrhage (ICH)^[2]
Dosage:	2% solution; 4 mL/kg
Administration:	Jugular vein or intraperitoneal injection; once
Result:	Showed the amount of stain accumulated in the brains.

Clinical Trial

Molecular Weight

960.81

Formula

C₃₄H₂₄N₆Na₄O₁₄S₄

CAS No.

314-13-6

Appearance

Solid

Color

Brown to black

SMILES

CC1=CC(C2=CC=C(/N=N/C3=C(O)C4=C(N)C(S(=O)(O[Na])=O)=CC(S(=O)(O[Na])=O)=C4C=C3)C(C)=C2)=CC=C1/N=N/C5=C(O)C6=C(N)C(S(=O)(O[Na])=O)=CC(S(=O)(O[Na])=O)=C6C=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 31.25 mg/mL (32.52 mM; Need ultrasonic)

DMSO : ≥ 25 mg/mL (26.02 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.0408 mL	5.2039 mL	10.4079 mL
5 mM	0.2082 mL	1.0408 mL	2.0816 mL
10 mM	0.1041 mL	0.5204 mL	1.0408 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (2.60 mM); Clear solution; Need warming

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.86%

Select Batch:

Data Sheet (275 KB) SDS (420 KB)

COA (250 KB) HNMR (354 KB) RP-HPLC (230 KB)

Handling Instructions (2659 KB)

References

[1]. Linpeng Yao, et al. Evans Blue Dye: A Revisit of Its Applications in Biomedicine. Contrast Media Mol Imaging. 2018 Apr 22:2018:7628037. [Content Brief]

[2]. Anatol Manaenko, et al. Comparison Evans Blue injection routes: Intravenous versus intraperitoneal, for measurement of blood-brain barrier in a mice hemorrhage model. J Neurosci Methods. 2011 Feb 15;195(2):206-10. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	1.0408 mL	5.2039 mL	10.4079 mL	26.0197 mL
	5 mM	0.2082 mL	1.0408 mL	2.0816 mL	5.2039 mL
	10 mM	0.1041 mL	0.5204 mL	1.0408 mL	2.6020 mL
	15 mM	0.0694 mL	0.3469 mL	0.6939 mL	1.7346 mL
	20 mM	0.0520 mL	0.2602 mL	0.5204 mL	1.3010 mL
	25 mM	0.0416 mL	0.2082 mL	0.4163 mL	1.0408 mL
H₂O	30 mM	0.0347 mL	0.1735 mL	0.3469 mL	0.8673 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Evans Blue Related Classifications

Neurological Disease Cardiovascular Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Evans Blue314-13-6Direct Blue 53 T-1824 C.I. 23860Direct Blue53Direct Blue-53T1824T 1824T-1824EAATiGluRExcitatory amino acid transporter Glutamate transporterIonotropic glutamate receptorsInhibitorinhibitorinhibit

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Evans Blue (Synonyms: Direct Blue 53; T-1824; C.I. 23860)

Customer Review

Other Forms of Evans Blue:

Evans Blue Related Antibodies

29 Publications Citing Use of MCE Evans Blue

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All EAAT Isoform Specific Products:

View All iGluR Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Evans Blue Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Evans Blue Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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