2. Apoptosis
  3. Apoptosis
  4. Ubiquitin Isopeptidase Inhibitor I, G5

Ubiquitin Isopeptidase Inhibitor I, G5  (Synonyms: NSC144303)

製品番号: HY-100738 純度: 98.0%
COA 取扱説明書 Technical Support

Ubiquitin Isopeptidase Inhibitor I, G5 (NSC 144303) is an apoptosome-independent caspase and apoptosis activator with IC50 values of 1.76 and 1.6 μM in E1A and E1A/C9DN cells, respectively.

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Ubiquitin Isopeptidase Inhibitor I, G5

Ubiquitin Isopeptidase Inhibitor I, G5 構造式

CAS 番号 : 108477-18-5

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>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
5 mg $92 在庫あり
10 mg $158 在庫あり
25 mg $310 在庫あり
50 mg $500 在庫あり
100 mg $800 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

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Based on 7 publication(s) in Google Scholar

Other Forms of Ubiquitin Isopeptidase Inhibitor I, G5:

Ubiquitin Isopeptidase Inhibitor I, G5 関連抗体

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製品説明

Ubiquitin Isopeptidase Inhibitor I, G5 (NSC 144303) is an apoptosome-independent caspase and apoptosis activator with IC50 values of 1.76 and 1.6 μM in E1A and E1A/C9DN cells, respectively.

IC50 & Target

IC50: 1.76 μM (E1A cells), 1.6 μM (E1A/C9DN cells)[1]
Cellular Effect
Cell Line Type Value Description References
Sf9 IC50
33.23 μM
Compound: G5
Inhibition of recombinant human USP18 (16 to 372 aa) expressed in Sf9 cells using ISG15-AMC as substrate preincubated for 30 mins before substrate addition measured after 60 mins by fluorescence assay
Inhibition of recombinant human USP18 (16 to 372 aa) expressed in Sf9 cells using ISG15-AMC as substrate preincubated for 30 mins before substrate addition measured after 60 mins by fluorescence assay
[PMID: 25639862]
U-87MG ATCC IC50
0.77 μM
Compound: G5
Cytotoxicity against human U87MG cells after 48 hrs by resazurin assay
Cytotoxicity against human U87MG cells after 48 hrs by resazurin assay
[PMID: 25639862]
体外実験

G5 is capable of activating an apoptosome-independent apoptotic pathway. It targets the ubiquitinproteasome system by inhibiting the ubiquitin isopeptidases. G5 induces a rather unique apoptotic pathway, which includes a Bcl-2-dependent but apoptosome-independent mitochondrial pathway with upregulation of the BH3-only protein Noxa, stabilization of the inhibitor of apoptosis antagonist Smac, but also the involvement of the death receptor pathway. G5 is a potent apoptotic inducer showing IC50s of 1.76 and 1.6 μM in E1A and E1A/C9DN cells, respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ubiquitin Isopeptidase Inhibitor I, G5 関連抗体
分子量

414.39

分子式

C19H14N2O7S
CAS 番号
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(/C(C1)=C/C2=CC=C([N+]([O-])=O)C=C2)/C(CS1(=O)=O)=C/C3=CC=C([N+]([O-])=O)C=C3
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years

* The compound is unstable in solutions, freshly prepared is recommended.

溶剤 & 溶解度
体外: 

DMSO : 25 mg/mL (60.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

THF : 3.44 mg/mL (8.30 mM; Need ultrasonic)

Acetone : 2.86 mg/mL (6.90 mM; Need ultrasonic)

Ethanol : < 1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4132 mL 12.0659 mL 24.1319 mL
5 mM 0.4826 mL 2.4132 mL 4.8264 mL
10 mM 0.2413 mL 1.2066 mL 2.4132 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  15% Cremophor EL    85% Saline

    Solubility: 10 mg/mL (24.13 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
純度とドキュメンテーション

純度: 98.0%

COA (246 KB) HNMR (249 KB)

取扱説明書 (2659 KB)
参考文献
細胞実験
[1]

Different drug concentrations are used to determine the dose-response profile and to calculate the IC50 value. Cells are incubated with the required concentrations of the 57 compounds. After 24 or 48 hours of incubation, cell death is evaluated bytr ypan blue staining[1].

MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Acetone / THF / DMSO 1 mM 2.4132 mL 12.0659 mL 24.1319 mL 60.3296 mL
5 mM 0.4826 mL 2.4132 mL 4.8264 mL 12.0659 mL
DMSO 10 mM 0.2413 mL 1.2066 mL 2.4132 mL 6.0330 mL
15 mM 0.1609 mL 0.8044 mL 1.6088 mL 4.0220 mL
20 mM 0.1207 mL 0.6033 mL 1.2066 mL 3.0165 mL
25 mM 0.0965 mL 0.4826 mL 0.9653 mL 2.4132 mL
30 mM 0.0804 mL 0.4022 mL 0.8044 mL 2.0110 mL
40 mM 0.0603 mL 0.3016 mL 0.6033 mL 1.5082 mL
50 mM 0.0483 mL 0.2413 mL 0.4826 mL 1.2066 mL
60 mM 0.0402 mL 0.2011 mL 0.4022 mL 1.0055 mL
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Ubiquitin Isopeptidase Inhibitor I, G5 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ubiquitin Isopeptidase Inhibitor I, G5108477-18-5NSC144303NSC 144303NSC-144303ApoptosisInhibitorinhibitorinhibit

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製品名:
Ubiquitin Isopeptidase Inhibitor I, G5
製品番号:
HY-100738
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