2. Immunology/Inflammation
  3. PGE synthase
  4. ADP-β-S trisodium

ADP-β-S trisodium  (Synonyms: Adenosine 5'-(β-thiodiphosphate) trisodium)

Cat. No.: HY-134353A
Handling Instructions Technical Support

ADP-β-S (Adenosine 5'-(β-thiodiphosphate)) trilithium is a non-hydrolyzable ADP analog and a P2Y12 receptor agonist. ADP-β-S trilithium activates the P2Y12 receptor in microglia, thereby triggering downstream inflammatory signaling pathways. ADP-β-S trilithium activates P2Y purinergic receptors in rat pancreatic β cells and enhances glucose-induced insulin secretion. ADP-β-S trilithium can be used in the research of diseases such as inflammation and diabetes.

For research use only. We do not sell to patients.

ADP-β-S trisodium

ADP-β-S trisodium Chemical Structure

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ADP-β-S trisodium Related Antibodies

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Description

ADP-β-S (Adenosine 5'-(β-thiodiphosphate)) trilithium is a non-hydrolyzable ADP analog and a P2Y12 receptor agonist. ADP-β-S trilithium activates the P2Y12 receptor in microglia, thereby triggering downstream inflammatory signaling pathways. ADP-β-S trilithium activates P2Y purinergic receptors in rat pancreatic β cells and enhances glucose-induced insulin secretion. ADP-β-S trilithium can be used in the research of diseases such as inflammation and diabetes[1][2].

In Vitro

ADP-β-S (0.2-2 mM; 30 min) trilithium enhances IL-1β production in primary cultured murine microglia via the P2Y12 receptor, with significant effects observed at 0.2 mM and 2 mM concentrations when paired with LPS or LPS plus ATP stimulation[1].
ADP-β-S (0.2 mM; 30 min) trilithium enhances LPS plus ATP-induced IL-1β production in wild-type MG6 murine microglial cells, but not in P2ry12 KO MG6 cells, confirming dependence on the P2Y12 receptor[1].
ADP-β-S (0.2 mM; 24 h) trilithium enhances LPS-induced IL-6 production in MG6 murine microglial cells via the P2Y12 receptor[1].
ADP-β-S (0.2 mM; 24 h) trilithium enhances LPS-induced IL-6 production in primary cultured murine microglia via the P2Y12 receptor[1].
ADP-β-S (0.2 mM; 24 h) trilithium enhances LPS-induced IL-6 production in wild-type MG6 murine microglial cells, but not in P2ry12 KO MG6 cells, confirming dependence on the P2Y12 receptor[1].
ADP-β-S (0.2 mM; 24 h) trilithium potentiates LPS-induced NF-κB activation, including nuclear translocation and phosphorylation, in MG6 murine microglial cells[1].
ADP-β-S (trilithium) (0.2-2 mM; 30 min) activates caspase-1 at 2 mM and enhances LPS plus ATP-induced caspase-1 activation at 0.2 mM in MG6 murine microglial cells[1].
ADP-β-S (0.2 mM; 30 min) trilithium enhances LPS plus ATP-induced mitochondrial membrane potential disruption in MG6 murine microglial cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ADP-β-S trisodium Related Antibodies

ELISA Assay[1]

Cell Line: primary cultured murine microglia
Concentration: 0.2 mM, 2 mM
Incubation Time: 30 min
Result: Induced significant IL-1β release when paired with LPS at 2 mM, which was partially blocked by PSB0739.
Induced modest but significant IL-1β release when paired with LPS at 0.2 mM.
Significantly augmented ATP-induced IL-1β production when added alongside LPS plus 1 or 2 mM ATP at 0.2 mM, and this augmentation was suppressed by PSB0739.

ELISA Assay[1]

Cell Line: wild-type and P2ry12 KO MG6 immortalized murine microglial cells
Concentration: 0.2 mM
Incubation Time: 30 min
Result: Significantly increased IL-1β production in wild-type MG6 cells stimulated with LPS plus ATP.
Had no effect on IL-1β production in P2ry12 KO MG6 cells under the same conditions.

ELISA Assay[1]

Cell Line: MG6 immortalized murine microglial cells
Concentration: 0.2 mM
Incubation Time: 24 h
Result: Significantly increased LPS-induced IL-6 production in MG6 cells, and this increase was abrogated by the P2Y12 receptor antagonist PSB0739.

ELISA Assay[1]

Cell Line: primary cultured murine microglia
Concentration: 0.2 mM
Incubation Time: 24 h
Result: Significantly increased LPS-induced IL-6 production in primary microglia, and this increase was reduced by the P2Y12 receptor antagonist PSB0739.

ELISA Assay[1]

Cell Line: wild-type and P2ry12 KO MG6 immortalized murine microglial cells
Concentration: 0.2 mM
Incubation Time: 24 h
Result: Significantly increased LPS-induced IL-6 production in wild-type MG6 cells.
Had no effect on IL-6 production in P2ry12 KO MG6 cells under the same conditions.

Western Blot Analysis[1]

Cell Line: MG6 immortalized murine microglial cells
Concentration: 0.2 mM, 2 mM
Incubation Time: 30 min
Result: Activated caspase-1 when paired with LPS at 2 mM, as shown by presence of cleaved caspase-1 in culture supernatants.
Strongly enhanced caspase-1 activation when paired with LPS plus 1 mM ATP at 0.2 mM compared to LPS plus ATP alone.
Molecular Weight

509.21

Formula

C10H12N5Na3O9P2S
SMILES

O[C@H]1[C@@H](O)[C@H](N2C=NC3=C(N=CN=C32)N)O[C@@H]1COP(OP(O[Na])(S[Na])=O)(O[Na])=O
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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ADP-β-S trisodium Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ADP-β-S trisodiumAdenosine 5'-β-thiodiphosphate trisodiumPGE synthaseProstaglandin E synthaseADPadenylate cyclaseplateletInhibitorinhibitorinhibit

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