ADP-β-S trisodium
ADP-β-S (Adenosine 5'-(β-thiodiphosphate)) trilithium is a non-hydrolyzable ADP analog and a P2Y12 receptor agonist. ADP-β-S trilithium activates the P2Y12 receptor in microglia, thereby triggering downstream inflammatory signaling pathways. ADP-β-S trilithium activates P2Y purinergic receptors in rat pancreatic β cells and enhances glucose-induced insulin secretion. ADP-β-S trilithium can be used in the research of diseases such as inflammation and diabetes.
For research use only. We do not sell to patients.
- Formula: C10H12N5Na3O9P2S
- Molecular Weight:509.21
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
ADP-β-S (0.2-2 mM; 30 min) trilithium enhances IL-1β production in primary cultured murine microglia via the P2Y12 receptor, with significant effects observed at 0.2 mM and 2 mM concentrations when paired with LPS or LPS plus ATP stimulation[1].
ADP-β-S (0.2 mM; 30 min) trilithium enhances LPS plus ATP-induced IL-1β production in wild-type MG6 murine microglial cells, but not in P2ry12 KO MG6 cells, confirming dependence on the P2Y12 receptor[1].
ADP-β-S (0.2 mM; 24 h) trilithium enhances LPS-induced IL-6 production in MG6 murine microglial cells via the P2Y12 receptor[1].
ADP-β-S (0.2 mM; 24 h) trilithium enhances LPS-induced IL-6 production in primary cultured murine microglia via the P2Y12 receptor[1].
ADP-β-S (0.2 mM; 24 h) trilithium enhances LPS-induced IL-6 production in wild-type MG6 murine microglial cells, but not in P2ry12 KO MG6 cells, confirming dependence on the P2Y12 receptor[1].
ADP-β-S (0.2 mM; 24 h) trilithium potentiates LPS-induced NF-κB activation, including nuclear translocation and phosphorylation, in MG6 murine microglial cells[1].
ADP-β-S (trilithium) (0.2-2 mM; 30 min) activates caspase-1 at 2 mM and enhances LPS plus ATP-induced caspase-1 activation at 0.2 mM in MG6 murine microglial cells[1].
ADP-β-S (0.2 mM; 30 min) trilithium enhances LPS plus ATP-induced mitochondrial membrane potential disruption in MG6 murine microglial cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:primary cultured murine microglia
-
Concentration:0.2 mM, 2 mM
-
Incubation Time:30 min
-
Result:Induced significant IL-1β release when paired with LPS at 2 mM, which was partially blocked by PSB0739.
Induced modest but significant IL-1β release when paired with LPS at 0.2 mM.
Significantly augmented ATP-induced IL-1β production when added alongside LPS plus 1 or 2 mM ATP at 0.2 mM, and this augmentation was suppressed by PSB0739.
-
Cell Line:wild-type and P2ry12 KO MG6 immortalized murine microglial cells
-
Concentration:0.2 mM
-
Incubation Time:30 min
-
Result:Significantly increased IL-1β production in wild-type MG6 cells stimulated with LPS plus ATP.
Had no effect on IL-1β production in P2ry12 KO MG6 cells under the same conditions.
-
Cell Line:MG6 immortalized murine microglial cells
-
Concentration:0.2 mM
-
Incubation Time:24 h
-
Result:Significantly increased LPS-induced IL-6 production in MG6 cells, and this increase was abrogated by the P2Y12 receptor antagonist PSB0739.
-
Cell Line:primary cultured murine microglia
-
Concentration:0.2 mM
-
Incubation Time:24 h
-
Result:Significantly increased LPS-induced IL-6 production in primary microglia, and this increase was reduced by the P2Y12 receptor antagonist PSB0739.
-
Cell Line:wild-type and P2ry12 KO MG6 immortalized murine microglial cells
-
Concentration:0.2 mM
-
Incubation Time:24 h
-
Result:Significantly increased LPS-induced IL-6 production in wild-type MG6 cells.
Had no effect on IL-6 production in P2ry12 KO MG6 cells under the same conditions.
-
Cell Line:MG6 immortalized murine microglial cells
-
Concentration:0.2 mM, 2 mM
-
Incubation Time:30 min
-
Result:Activated caspase-1 when paired with LPS at 2 mM, as shown by presence of cleaved caspase-1 in culture supernatants.
Strongly enhanced caspase-1 activation when paired with LPS plus 1 mM ATP at 0.2 mM compared to LPS plus ATP alone.
Chemical Information
-
Molecular Weight 509.21
-
Formula C10H12N5Na3O9P2S
-
SMILES
O[C@H]1[C@@H](O)[C@H](N2C=NC3=C(N=CN=C32)N)O[C@@H]1COP(OP(O[Na])(S[Na])=O)(O[Na])=O
-
Synonyms
Adenosine 5'-(β-thiodiphosphate) trisodium
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Suzuki T, et al. Extracellular ADP augments microglial inflammasome and NF-κB activation via the P2Y12 receptor. Eur J Immunol. 2020;50(2):205-219. [Content Brief]
[2]. Bertrand G, et al. Adenosine-5'-O-(2-thiodiphosphate) is a potent agonist at P2 purinoceptors mediating insulin secretion from perfused rat pancreas. Br J Pharmacol. 1991;102(3):627-630. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
ADP-β-S trisodium Related Classifications
HY-134353A Related Classifications
Powered by Bioz