ADP-β-S trilithium  (Synonyms: Adenosine 5'-β-thiodiphosphate trilithium)

ADP-β-S (Adenosine 5'-(β-thiodiphosphate)) trilithium is a non-hydrolyzable ADP analog and a P2Y12 receptor agonist. ADP-β-S trilithium activates the P2Y12 receptor in microglia, thereby triggering downstream inflammatory signaling pathways. ADP-β-S trilithium activates P2Y purinergic receptors in rat pancreatic β cells and enhances glucose-induced insulin secretion. ADP-β-S trilithium can be used in the research of diseases such as inflammation and diabetes^[1][2].

ADP-β-S (0.2-2 mM; 30 min) trilithium enhances IL-1β production in primary cultured murine microglia via the P2Y12 receptor, with significant effects observed at 0.2 mM and 2 mM concentrations when paired with LPS or LPS plus ATP stimulation^[1].
ADP-β-S (0.2 mM; 30 min) trilithium enhances LPS plus ATP-induced IL-1β production in wild-type MG6 murine microglial cells, but not in P2ry12 KO MG6 cells, confirming dependence on the P2Y12 receptor^[1].
ADP-β-S (0.2 mM; 24 h) trilithium enhances LPS-induced IL-6 production in MG6 murine microglial cells via the P2Y12 receptor^[1].
ADP-β-S (0.2 mM; 24 h) trilithium enhances LPS-induced IL-6 production in primary cultured murine microglia via the P2Y12 receptor^[1].
ADP-β-S (0.2 mM; 24 h) trilithium enhances LPS-induced IL-6 production in wild-type MG6 murine microglial cells, but not in P2ry12 KO MG6 cells, confirming dependence on the P2Y12 receptor^[1].
ADP-β-S (0.2 mM; 24 h) trilithium potentiates LPS-induced NF-κB activation, including nuclear translocation and phosphorylation, in MG6 murine microglial cells^[1].
ADP-β-S (trilithium) (0.2-2 mM; 30 min) activates caspase-1 at 2 mM and enhances LPS plus ATP-induced caspase-1 activation at 0.2 mM in MG6 murine microglial cells^[1].
ADP-β-S (0.2 mM; 30 min) trilithium enhances LPS plus ATP-induced mitochondrial membrane potential disruption in MG6 murine microglial cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

461.07

C₁₀H₁₂Li₃N₅O₉P₂S

73536-95-5

Solid

White to off-white

O[C@H]1[C@@H](O)[C@H](N2C=NC3=C(N=CN=C32)N)O[C@@H]1COP(OP(O[Li])(S[Li])=O)(O[Li])=O

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Suzuki T, et al. Extracellular ADP augments microglial inflammasome and NF-κB activation via the P2Y12 receptor. Eur J Immunol. 2020;50(2):205-219.  [Content Brief]

  [2]. Bertrand G, et al. Adenosine-5'-O-(2-thiodiphosphate) is a potent agonist at P2 purinoceptors mediating insulin secretion from perfused rat pancreas. Br J Pharmacol. 1991;102(3):627-630.  [Content Brief]