Anti-CD24 Antibody (SWA11)
Based on 1 Customer Validation
For research use only. We do not sell to patients.
- Purity: 99.18%
- Molecular Weight:146.67 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Human IgG1 kappa
Human
CD24
Anti-CD24 Antibody (SWA11) inhibits the proliferation of human pancreatic cancer cells, ovarian cancer cells and lung cancer cells; it enhances the anti-tumor efficacy of Gemcitabine (HY-17026), 5-Fluorouridine (HY-107856), Oxaliplatin (HY-17371), Doxorubicin (HY-15142A), Paclitaxel (HY-B0015) and Irinotecan (HY-16562), and also enhances the anti-tumor efficacy of Cisplatin (HY-17394) in human embryonic carcinoma cells[2].
Anti-CD24 Antibody (SWA11) binds to CD24 on the surface of CD24-positive tumor cells, thereby triggering endocytosis, regulating STAT3-dependent gene expression and Src phosphorylation, directly inhibiting the proliferation, adhesion and invasion abilities of tumor cells. Meanwhile, it activates antibody-dependent cell-mediated cytotoxicity (ADCC) and the complement system to enhance anti-tumor efficacy[2].
Anti-CD24 Antibody (SWA11) conjugated with doxorubicin, namely SWA11-DOX, can be rapidly internalized by small cell lung cancer cells[2].
Anti-CD24 Antibody (SWA11) (1 μg/mL) specifically detects CD24 protein in human colorectal cancer HT-29 cell lysates[3].
Anti-CD24 Antibody (SWA11) (1.6-16 μg/mL; 1 h) selectively binds to CD24-positive human colorectal cancer cell line HT-29, while showing no significant binding to CD24-negative human colorectal cancer cell line HCT116[3].
Anti-CD24 Antibody (SWA11) (10 μg/mL; 30 min) shows specific binding to CD24-positive human colorectal cancer cells, which can be detected by flow cytometry[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HT-29 (CD24-positive), HCT116 (CD24-negative) human CRC cell lines
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Concentration:1.6, 3.2, 8, 16 μg/mL
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Incubation Time:1 h
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Result:Measured high absorbance in HT-29 cells across all tested concentrations.
Showed negligible absorbance in HCT116 cells across all tested concentrations.
Anti-CD24 Antibody (SWA11) (10 mg/kg; twice weekly; 4 weeks) reduces SKOV3ip ovarian carcinoma xenograft endpoint weight by relative to controls, decreases tumor cell proliferation and blood vessel density, and increases macrophage infiltration in female athymic CD1 nu/nu mice[1].
Anti-CD24 Antibody (SWA11) (10 mg/kg; once per treatment cycle, 3 total cycles, 1 day prior to Gemcitabine (HY-17026)) potentiates the anti-tumor efficacy of gemcitabine, reducing A549 lung carcinoma xenograft growth in female SCID beige mice[1].
Anti-CD24 Antibody (SWA11) slows tumor growth in human colorectal cancer xenografts, modulates the tumor immune microenvironment, and enhances the anti-tumor efficacy of several chemotherapy agents[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:SCID beige (female, 4- to 5-week-old; subcutaneous xenograft of A549 human lung adenocarcinoma cells)[1]
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Dosage:10 mg/kg
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Administration:4 times with 5-day interval
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Result:Reduced relative tumor volume increase by day 21 after first injection.
Reduced percentage of Ki-67-positive proliferating tumor cells.
Increased intratumoral levels of CCL1, CCL2/MCP-1, CCL12, CXCL9/MIG, CXCL10, CXCL11, IFN-γ, IL-1α, IL-1β, and IL-1Ra.
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Animal Model:athymic CD1 nu/nu (female; intraperitoneal inoculation of luciferase-transfected SKOV3ip human ovarian carcinoma cells)[1]
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Dosage:10 mg/kg
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Administration:8 times; twice weekly for 4 weeks
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Result:Reduced endpoint tumor weight.
Reduced percentage of Ki-67-positive proliferating tumor cells.
Increased intratumoral levels of CCL13, sICAM-1, IFN-γ, IL-1α, IL-1Ra, IL-12 p70, IL-16, IL-17, CXCL9/MIG, and CCL5/RANTES.
Increased infiltration of SKOV3ip tumors by F4/80-positive macrophages.
P25063-1
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
ELISA, FACS, Functional assay
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Flow cytometric analysis of 1×106 MCF-7 cells with Anti-CD24 Antibody (SWA11) (HY-P991196, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa Isotype Control (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Chemical Information
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Appearance Liquid
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Molecular Weight 146.67 kDa
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Color Colorless to light yellow
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SMILES
[Anti-CD24 Antibody (SWA11)]
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (264 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Salnikov AV, et al. Antibody targeting of CD24 efficiently retards growth and influences cytokine milieu in experimental carcinomas. Br J Cancer. 2013;108(7):1449-1459. [Content Brief]
[2]. Huang S, et al. Checkpoint CD24 function on tumor and immunotherapy. Front Immunol. 2024;15:1367959. [Content Brief]
[3]. Shapira S, et al. An immunoconjugate of anti-CD24 and Pseudomonas exotoxin selectively kills human colorectal tumors in mice. Gastroenterology. 2011;140(3):935-946. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-CD24 Antibody (SWA11) Related Classifications
HY-P991196 Related Classifications
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