Anti-Mouse IFN gamma Antibody (H22) 

Anti-Mouse IFN gamma Antibody (H22) is an anti-mouse IFN gamma IgG antibody inhibitor derived from host Armenian Hamster. Anti-Mouse IFN gamma Antibody (H22) abrogates specific binding of ^I25II-rIFN-γ to macrophages. Anti-Mouse IFN gamma Antibody (H22) inhibits macrophage-activating factor (MAF) (ID₅₀ = 56 ng). Anti-Mouse IFN gamma Antibody (H22) inhibits IFN-gamma dependent antiviral activity (ID₅₀ = 2 ng). Anti-Mouse IFN gamma Antibody (H22) neutralizes IFNγ in mice. Anti-Mouse IFN gamma Antibody (H22) can be used for the researches of inflammation and infection, such as arthritis^[1][2][3][4].

Armenian Hamster IgG

Polyclonal Armenian hamster IgG, Isotype Control

Mouse

IFN gamma

Anti-Mouse IFN gamma Antibody (H22) (1 h) inhibits both macrophage-activating factor (MAF) and antiviral activities with ID₅₀ of 56 ng and 2 ng^[4].
Anti-Mouse IFN gamma Antibody (H22) (50 ng, 2 h) abrogates specific binding of ^I25II-rIFN-γ to macrophages^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-Mouse IFN gamma Antibody (H22) (200 μg, at days on 0, 1, 4, 11 and 18 for 5 times) neutralizes IFNγ in mice^[1].
Anti-Mouse IFN gamma Antibody (H22) (250 μg, i.p., at days 0, 6, 13 21, 28, 35 and 42 for 7 times) highly increases the number of limbs affected by arthritis in collagen-induced arthritis mice models^[2].
Anti-Mouse IFN gamma Antibody (H22) (100 μg, i.p., once weekly beginning 5 h before cell transfer) completely ablates the protective effect of Lyt-2+ immune T cells against toxoplasmosis in mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

15978  [NCBI]

P01580

ELISA, FACS, Functional assay, Research in vivo

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

150 kDa

Liquid

Colorless to light yellow

[Anti-Mouse IFN gamma Antibody (H22)]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Slaney CY, et al. Dual-specific Chimeric Antigen Receptor T Cells and an Indirect Vaccine Eradicate a Variety of Large Solid Tumors in an Immunocompetent, Self-antigen Setting. Clin Cancer Res. 2017 May 15;23(10):2478-2490.  [Content Brief]

  [2]. Williams RO, et al. Increased limb involvement in murine collagen-induced arthritis following treatment with anti-interferon-gamma. Clin Exp Immunol. 1993 May;92(2):323-7.  [Content Brief]

  [3]. uzuki Y, et al. The effect of anti-IFN-gamma antibody on the protective effect of Lyt-2+ immune T cells against toxoplasmosis in mice. J Immunol. 1990 Mar 1;144(5):1954-6.  [Content Brief]

  [4]. Schreiber RD, et al. Monoclonal antibodies to murine gamma-interferon which differentially modulate macrophage activation and antiviral activity. J Immunol. 1985 Mar;134(3):1609-18.  [Content Brief]