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Folate Binding Protein Antibody (YA3809)

(Synonyms: FBP; FOLR)
Customer Review

Based on 1 Customer Validation

Folate Binding Protein Antibody (YA3809) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Folate Binding Protein.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2a

  • Application:

    IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Folate Binding Protein Antibody (YA3809)
  • Experimental Validation Results for Folate Binding Protein Antibody (YA3809)
  • Folate Binding Protein Antibody (YA3809) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:100-1:500 1:200-1:400 1:10000

Product Details

Description

Folate Binding Protein Antibody (YA3809) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Folate Binding Protein.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 40 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 30 kDa
Immunogen

Purified recombinant fragment of human FOLR1 (AA: 41-227) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2a

RRID

AB_3718838

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Folate Binding Protein Antibody (YA3809)
    Immunocytochemistry analysis of MCF-7 cells labeling Folate Binding Protein with Folate Binding Protein Antibody (HY-P84112) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Folate Binding Protein Antibody (HY-P84112) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Folate Binding Protein Antibody (YA3809)
    Immunocytochemistry analysis of MCF-7 cells labeling Folate Binding Protein with Folate Binding Protein Antibody (HY-P84112) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Folate Binding Protein Antibody (HY-P84112) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Folate Binding Protein binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. Required for normal embryonic development and normal cell proliferation[1][2][3][4][5][6].

  • Subcellular Localization

    Cell membrane; Lipid-anchor, GPI-anchor; Apical cell membrane; Lipid-anchor, GPI-anchor; Basolateral cell membrane; Lipid-anchor, GPI-like-anchor; Secreted; Cytoplasmic vesicle; Cytoplasmic vesicle, clathrin-coated vesicle; Endosome

  • Expression


    Tissue_specificity:It is primarily expressed in epithelial tissues. Expression increases in malignant tissues. It is expressed in the kidneys, lungs, and cerebellum. It can also be detected in the placental and thymic epithelium.

  • SwissProt ID

    P15328

  • Gene ID
    2348 [NCBI]
  • Synonyms

    FBP; FOLR

Folate Binding Protein Antibody (YA3809) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Folate Binding Protein with Folate Binding Protein Antibody (HY-P84112) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Folate Binding Protein Antibody (HY-P84112) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Folate Binding Protein with Folate Binding Protein Antibody (HY-P84112) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Folate Binding Protein Antibody (HY-P84112) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Folate Binding Protein Antibody (YA3809) Product Image
    1/16
  • Experimental Validation Results for Folate Binding Protein Antibody (YA3809)
  • Experimental Validation Results for Folate Binding Protein Antibody (YA3809)
  • Folate Binding Protein Antibody (YA3809) Product Image
  • Experimental Validation Results for Folate Binding Protein Antibody (YA3809)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Folate Binding Protein with Folate Binding Protein Antibody (HY-P84112) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Folate Binding Protein Antibody (HY-P84112) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Folate Binding Protein Antibody (YA3809)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Folate Binding Protein with Folate Binding Protein Antibody (HY-P84112) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Folate Binding Protein Antibody (HY-P84112) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Folate Binding Protein Antibody (YA3809)
  • Experimental Validation Results for Folate Binding Protein Antibody (YA3809)
  • Folate Binding Protein Antibody (YA3809) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling Folate Binding Protein with Folate Binding Protein Antibody (HY-P84112) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Folate Binding Protein Antibody (HY-P84112) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Folate Binding Protein Antibody (YA3809) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling Folate Binding Protein with Folate Binding Protein Antibody (HY-P84112) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Folate Binding Protein Antibody (HY-P84112) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Folate Binding Protein Antibody (YA3809) Product Image ICC
  • Folate Binding Protein Antibody (YA3809) Product Image ICC