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Plexin A1 Antibody (YA4563)

(Synonyms: NOV; NOVP; PLXN1; PLEXIN-A1)
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Based on 1 Customer Validation

Plexin A1 Antibody (YA4563) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Plexin A1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Plexin A1 Antibody (YA4563)
  • Experimental Validation Results for Plexin A1 Antibody (YA4563)
  • Plexin A1 Antibody (YA4563) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:100-1:500 1:200-1:400 1:10000

Product Details

Description

Plexin A1 Antibody (YA4563) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Plexin A1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 211 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 211 kDa
Immunogen

Purified recombinant fragment of human PLXNA1 (AA: 1100-1200) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718992

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Plexin A1 Antibody (YA4563)
    Immunocytochemistry analysis of A431 cells labeling Plexin A1 with Plexin A1 Antibody (HY-P84866) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Plexin A1 Antibody (HY-P84866) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Plexin A1 Antibody (YA4563)
    Immunocytochemistry analysis of U2OS cells labeling Plexin A1 with Plexin A1 Antibody (HY-P84866) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Plexin A1 Antibody (HY-P84866) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Plexin A1 is a Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. Acts as coreceptor of TREM2 for SEMA6D in dendritic cells and is involved in the generation of immune responses and skeletal homeostasis

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:It was detected in the fetus's brain, lungs, liver, and kidneys.

  • Subunit

    Interacts directly with NRP1 and NRP2. Interacts with PLXN1B. Interacts with FARP2, RND1 and KDR/VEGFR2. Binding of SEMA3A leads to dissociation of FARP2. Interacts with CRMP1, DPYSL2/CRMP2, DPYSL3/CRMP3 and DPYSL4/CRMP4. Interacts (via TIG domains) with TREM2; the interaction mediates SEMA6D binding and signaling through TYROBP

  • SwissProt ID

    Q9UIW2

  • Gene ID
    5361 [NCBI]
  • Synonyms

    NOV; NOVP; PLXN1; PLEXIN-A1

Plexin A1 Antibody (YA4563) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling Plexin A1 with Plexin A1 Antibody (HY-P84866) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Plexin A1 Antibody (HY-P84866) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of U2OS cells labeling Plexin A1 with Plexin A1 Antibody (HY-P84866) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Plexin A1 Antibody (HY-P84866) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Plexin A1 Antibody (YA4563) Product Image
    1/16
  • Experimental Validation Results for Plexin A1 Antibody (YA4563)
  • Experimental Validation Results for Plexin A1 Antibody (YA4563)
  • Plexin A1 Antibody (YA4563) Product Image
  • Experimental Validation Results for Plexin A1 Antibody (YA4563)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling Plexin A1 with Plexin A1 Antibody (HY-P84866) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Plexin A1 Antibody (HY-P84866) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Plexin A1 Antibody (YA4563)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of U2OS cells labeling Plexin A1 with Plexin A1 Antibody (HY-P84866) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Plexin A1 Antibody (HY-P84866) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Plexin A1 Antibody (YA4563)
  • Experimental Validation Results for Plexin A1 Antibody (YA4563)
  • Plexin A1 Antibody (YA4563) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A431 cells labeling Plexin A1 with Plexin A1 Antibody (HY-P84866) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Plexin A1 Antibody (HY-P84866) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Plexin A1 Antibody (YA4563) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of U2OS cells labeling Plexin A1 with Plexin A1 Antibody (HY-P84866) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Plexin A1 Antibody (HY-P84866) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Plexin A1 Antibody (YA4563) Product Image ICC
  • Plexin A1 Antibody (YA4563) Product Image ICC