Betazole hydrochloride
Based on 1 publication(s) in Google Scholar
Betazole hydrochloride (Ametazole hydrochloride), a pyrazole analogue of histamine, is an orally active histamine H2 receptor agonist. Betazole induces gastric acid secretion and causes an immediate and significant increase in common bile duct pressure. Betazole is used as a diagnostic agent known as histalog for investigating gastric acid secretory capacity.
For research use only. We do not sell to patients.
- Purity : 98.48%
- CAS No.: 1121-45-5
- Formula: C5H10ClN3
- Molecular Weight:147.61
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Betazole hydrochloride
MoreAll Histamine Receptor Isoforms
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Biological Activity
Description
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H2 Receptor |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| Caco-2 | Inhibition |
-11.15 %
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Antiviral activity determined as inhibition of SARS-CoV-2 induced cytotoxicity of Caco-2 cells at 10 uM after 48 hours by high content imaging
Antiviral activity determined as inhibition of SARS-CoV-2 induced cytotoxicity of Caco-2 cells at 10 uM after 48 hours by high content imaging
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10.21203/rs.3.rs-23951/v1 |
Chemical Information
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CAS No. 1121-45-5
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Appearance Solid
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Molecular Weight 147.61
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Formula C5H10ClN3
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Color White to off-white
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SMILES
NCCC1=NNC=C1.Cl
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Synonyms
Ametazole hydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Proc Natl Acad Sci U S A
Histamine H2 receptor deficit in glutamatergic neurons contributes to the pathogenesis of schizophrenia. [Abstract]2023 Feb 28;120(9):e2207003120. PMID: 36812204
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (338.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (16.94 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (16.94 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Maples MD, Effects of betazole hydrochloride and cimetidine on common bile pressure and duodenal myoelectric activity in the dog. Am Surg. 1981;47(12):519-521. [Content Brief]
[2]. Spitz IM, Betazole-induced GIP secretion is not mediated by gastric HCl. Metabolism. 1982;31(4):380-382. [Content Brief]
[3]. CLAYMAN CB,. A simple oral gastric secretory stimulant (betazole hydrochloride). JAMA. 1961;175:908-909. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 6.7746 mL | 33.8730 mL | 67.7461 mL | 169.3652 mL |
| 5 mM | 1.3549 mL | 6.7746 mL | 13.5492 mL | 33.8730 mL | |
| 10 mM | 0.6775 mL | 3.3873 mL | 6.7746 mL | 16.9365 mL | |
| 15 mM | 0.4516 mL | 2.2582 mL | 4.5164 mL | 11.2910 mL | |
| 20 mM | 0.3387 mL | 1.6937 mL | 3.3873 mL | 8.4683 mL | |
| 25 mM | 0.2710 mL | 1.3549 mL | 2.7098 mL | 6.7746 mL | |
| 30 mM | 0.2258 mL | 1.1291 mL | 2.2582 mL | 5.6455 mL | |
| 40 mM | 0.1694 mL | 0.8468 mL | 1.6937 mL | 4.2341 mL | |
| 50 mM | 0.1355 mL | 0.6775 mL | 1.3549 mL | 3.3873 mL | |
| 60 mM | 0.1129 mL | 0.5646 mL | 1.1291 mL | 2.8228 mL | |
| 80 mM | 0.0847 mL | 0.4234 mL | 0.8468 mL | 2.1171 mL | |
| 100 mM | 0.0677 mL | 0.3387 mL | 0.6775 mL | 1.6937 mL |
Betazole hydrochloride Related Classifications
HY-B1557B Related Classifications
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