PRMT5:MEP50 PPI
Based on 1 Customer Validation
PRMT5:MEP50 PPI is a novel PRMT5:MEP50 protein-protein interaction (PRMT5:MEP50 PPI) inhibitor, shows anti-tumor activity and anti-proliferative activity of lung and prostate cancer cells.
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- Pureté: 98.09%
- CAS No.: 3031536-71-4
- Formule: C24H22N4O4
- Masse moléculaire:430.46
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Stockage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
447 nM
Compound: 17
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Cytotoxicity against human A549 cells after 72 hrs by MTT assay
Cytotoxicity against human A549 cells after 72 hrs by MTT assay
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[PMID: 36206451] |
| LNCaP | IC50 |
430 nM
Compound: 17
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Induction of cell death in human LNCaP cells incubated for 72 hrs by MTT assay
Induction of cell death in human LNCaP cells incubated for 72 hrs by MTT assay
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[PMID: 36206451] |
In Vitro
PRMT5:MEP50 PPI (0-10 μM; 72 h) inhibits the growth of prostate cancer cells[1].
PRMT5:MEP50 PPI (250-1000 nM; 72 h) decreases the expression of global arginine 3 residue of histone H4 (H4R3me2s) level in prostate cancer cells[1].
PRMT5:MEP50 PPI (500 nM; 72 h) de-represseses the PRMT5: MEP50-regulated IVL gene of prostate cancer cells[1].
PRMT5:MEP50 PPI treatment inhibits multiple PRMT5-regulated pathways critical to the survival and proliferation of lung and prostate cancer cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:LNCaP cells
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Concentration:0-10 μM
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Incubation Time:72 h
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Result:Showed IC50 value of 430.2 nM.
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Cell Line:LNCaP cells
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Concentration:250, 500 and 1000 nM
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Incubation Time:72 h
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Result:Observed global arginine 3 residue of histone H4 (H4R3me2s) decreased by 65%.
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Cell Line:LNCaP cells
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Concentration:500 nM
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Incubation Time:72 h
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Result:De-repressesed the PRMT5: MEP50-regulated IVL gene without significantly altering the expression of the PRMT5: pICln-regulated AR gene.
Chemical Information
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CAS No. 3031536-71-4
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Appearance Solid
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Masse moléculaire 430.46
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Formule C24H22N4O4
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Color White to off-white
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SMILES
CCC1=NOC(C)=C1COC2=CC(C(NNC(C3=NC4=C(C=C3)C=CC=C4)=O)=O)=CC=C2
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvant et solubilité
In Vitro:
DMSO : 200 mg/mL (464.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (11.62 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3231 mL | 11.6155 mL | 23.2310 mL | 58.0774 mL |
| 5 mM | 0.4646 mL | 2.3231 mL | 4.6462 mL | 11.6155 mL | |
| 10 mM | 0.2323 mL | 1.1615 mL | 2.3231 mL | 5.8077 mL | |
| 15 mM | 0.1549 mL | 0.7744 mL | 1.5487 mL | 3.8718 mL | |
| 20 mM | 0.1162 mL | 0.5808 mL | 1.1615 mL | 2.9039 mL | |
| 25 mM | 0.0929 mL | 0.4646 mL | 0.9292 mL | 2.3231 mL | |
| 30 mM | 0.0774 mL | 0.3872 mL | 0.7744 mL | 1.9359 mL | |
| 40 mM | 0.0581 mL | 0.2904 mL | 0.5808 mL | 1.4519 mL | |
| 50 mM | 0.0465 mL | 0.2323 mL | 0.4646 mL | 1.1615 mL | |
| 60 mM | 0.0387 mL | 0.1936 mL | 0.3872 mL | 0.9680 mL | |
| 80 mM | 0.0290 mL | 0.1452 mL | 0.2904 mL | 0.7260 mL | |
| 100 mM | 0.0232 mL | 0.1162 mL | 0.2323 mL | 0.5808 mL |
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