Art. -Nr.: HY-N0114

Evodiamine

(Synonyms: (+)-Evodiamine; d-Evodiamine)

19 Cited Publications

Kundenbewertung

Based on 19 publication(s) in Google Scholar

Evodiamine is an alkaloid isolated from the fruit of Evodia rutaecarpa Bentham with diverse biological activities including anti-inflammatory, anti-obesity, and antitumor.

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Reinheit: 99.97%
CAS. Nr.: 518-17-2
Formel: C₁₉H₁₇N₃O
Molecular Weight:303.36

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months

Evodiamine Chemical Structure

CAS. Nr.: 518-17-2

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Use of MCE Products

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (19)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Protokoll
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Evodiamine is an alkaloid isolated from the fruit of Evodia rutaecarpa Bentham with diverse biological activities including anti-inflammatory, anti-obesity, and antitumor.

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>200 μM Compound: 1	Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs by CCK8 assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs by CCK8 assay	[PMID: 31880942]
A549	IC₅₀	24.67 μM Compound: EVO	Antiproliferative activity against human A549 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 38244939]
A549	IC₅₀	9.19 μM Compound: EVO	Antiproliferative activity against human A549 cells assessed as inhibition of cell growth incubated for 48 hrs in presence of erlotinib by MTT assay Antiproliferative activity against human A549 cells assessed as inhibition of cell growth incubated for 48 hrs in presence of erlotinib by MTT assay	[PMID: 38244939]
HCT-116	IC₅₀	>200 μM Compound: 1	Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability incubated for 72 hrs by CCK8 assay Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability incubated for 72 hrs by CCK8 assay	[PMID: 31880942]
MCF7	IC₅₀	5.4 μM Compound: 6	Antiproliferative activity against human MCF7 cells after 48 to 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 48 to 72 hrs by MTT assay	10.1039/C6MD00170J
MDA-MB-435	IC₅₀	22.1 μM Compound: 1	Antiproliferative activity against human MDA-MB-435 cells assessed as reduction in cell viability incubated for 72 hrs by CCK8 assay Antiproliferative activity against human MDA-MB-435 cells assessed as reduction in cell viability incubated for 72 hrs by CCK8 assay	[PMID: 31880942]
MRC5	IC₅₀	>50 μM Compound: Evo	Cytotoxicity against human MRC5 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB assay Cytotoxicity against human MRC5 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB assay	[PMID: 39151060]
MSTO-211H	IC₅₀	0.54 μM Compound: 6	Antiproliferative activity against human MSTO-211H cells after 48 to 72 hrs by MTT assay Antiproliferative activity against human MSTO-211H cells after 48 to 72 hrs by MTT assay	10.1039/C6MD00170J
NCI-H460	IC₅₀	0.26 μM Compound: 6	Antiproliferative activity against human H460 cells after 48 to 72 hrs by MTT assay Antiproliferative activity against human H460 cells after 48 to 72 hrs by MTT assay	10.1039/C6MD00170J
NCI-H460	IC₅₀	13.44 μM Compound: EVO	Antiproliferative activity against human NCI-H460 cells assessed as inhibition of cell growth incubated for 48 hrs in presence of erlotinib by MTT assay Antiproliferative activity against human NCI-H460 cells assessed as inhibition of cell growth incubated for 48 hrs in presence of erlotinib by MTT assay	[PMID: 38244939]
NCI-H460	IC₅₀	17.14 μM Compound: EVO	Antiproliferative activity against human NCI-H460 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Antiproliferative activity against human NCI-H460 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 38244939]
NCI-H460	IC₅₀	2.6 μM Compound: 1a	Cytotoxicity against human H460 cells after 72 hrs by MTT assay Cytotoxicity against human H460 cells after 72 hrs by MTT assay	[PMID: 24103429]
PANC-1	IC₅₀	6.59 μM Compound: Evo	Cytotoxicity against human PANC-1 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB assay Cytotoxicity against human PANC-1 cells assessed as inhibition of cell growth incubated for 72 hrs by SRB assay	[PMID: 39151060]
PC-9	IC₅₀	10.22 μM Compound: EVO	Antiproliferative activity against human PC-9 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Antiproliferative activity against human PC-9 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 38244939]
PC-9	IC₅₀	2.37 μM Compound: EVO	Antiproliferative activity against human PC-9 cells assessed as inhibition of cell growth incubated for 48 hrs in presence of erlotinib by MTT assay Antiproliferative activity against human PC-9 cells assessed as inhibition of cell growth incubated for 48 hrs in presence of erlotinib by MTT assay	[PMID: 38244939]

In Vitro

Evodiamine shows cytotoxicity against a variety of human cancer cell-lines by inducing apoptosis. Moreover, it is a naturally multi-targeting antitumor molecule, which exerts the antitumor activity by various molecular mechanism such as caspase-dependent and -independent pathways, sphingomyelin pathway, calcium/JNK signaling, PI3K/Akt/caspase and Fas-L/NF-κB signaling pathways^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Evodiamine inhibits the metabolism of dapoxetine. Compared to the control group, the pharmacokinetic parameter of t1/2, AUC(0^-∞) and Tmax of dapoxetine in evodiamine group is significantly increased by 63.3%, 44.8% and 50.4%, respectively. Moreover, evodiamine has significantly decreased the pharmacokinetic parameter of t1/2 and AUC(0^-∞) of desmethyl dapoxetine^[2]. Evodiamine suppresses tumor growth in a subcutaneous H22 xenograft model. Evodiamine attenuates VEGF-induced angiogenesis in vivo^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 518-17-2
Appearance Solid
Molecular Weight 303.36
Formel C₁₉H₁₇N₃O
Color White to yellow
SMILES

O=C1C2=CC=CC=C2N(C)[C@](N1CC3)([H])C4=C3C5=CC=CC=C5N4
Synonyms

(+)-Evodiamine; d-Evodiamine
Structure Classification
- Alkaloids
- Piperidine Alkaloids
- Alkaloids
- Indole Alkaloids
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Publications (19)

All Cell Proliferation/Viability AssayIn Vivo Efficacy StudyHistological Imaging/Staining

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Journal Impact Factor

Signal Transduct Target Ther

Large-scale and high-resolution mass spectrometry-based proteomics defines molecular subtypes of nasopharyngeal carcinoma for therapeutic targeting. [Abstract]

2026 Jun 23;11(1):244. PMID: 42331772
Adv Funct Mater

2025 Jun 20.
J Nanobiotechnology

An oral nanocombinatorial agent exhibits pleiotropic improvement in diabetic nephropathy via modulation of the SCAP/SREBPs pathway. [Abstract]

2026 Mar 22;24(1):405. PMID: 41866523

Evodiamine purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2026 Mar 22;24(1):405. [Abstract]

Cal27 and SAS cell viability of tumor cells pretreated by Evodiamine (EVO; 2-14 μg/mL), GTE, and GRE with different concentrations of 24 h.

Evodiamine purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2026 Mar 22;24(1):405. [Abstract]

In vivo tumor growth curves of SAS tumor-bearing mice treated with different formulations. Evodiamine (EVO, 4 mg/kg; i.v.; every other day for a total of 6 times) effectively inhibited tumor growth in a mouse oral squamous cell carcinoma model.

Evodiamine purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2026 Mar 22;24(1):405. [Abstract]

Immunohistochemical analyses of H&E, TUNEL, caspase9, caspase3, Ki67, and Hsp70 for tumor tissues after the last treatment with different formulations in vivo. The results showed that Evodiamine (EVO, 4 mg/kg; i.v.; once every other day for a total of 6 times; mice) confirmed the presence of apoptosis in the tumors of the EVO-treated group. Scale bar: 50 µm.
Pharmacol Res

Cardamonin retards progression of autosomal dominant polycystic kidney disease via inhibiting renal cyst growth and interstitial fibrosis. [Abstract]

2020 May;155:104751. PMID: 32151678
View

2025 Oct 28.

Evodiamine purchased from MedChemExpress. Usage Cited in: View. 2025 Oct 28.

Cell viability assay of MOVAS treated with Ang II and three candidate inhibitors (n = 3). The results showed that Evodiamine (1 µM; 6 h) significantly inhibited Ang II-induced SMC proliferation.
Phytomedicine

Evodiamine inhibits ESCC by inducing M-phase cell-cycle arrest via CUL4A/p53/p21 axis and activating noxa-dependent intrinsic and DR4-dependent extrinsic apoptosis. [Abstract]

2023 Jan:108:154493. PMID: 36265256
J Transl Med

Evodiamine inhibits proliferation and induces apoptosis of nasopharyngeal carcinoma cells via the SRC/ERBB2-mediated MAPK/ERK signaling pathway. [Abstract]

2024 Sep 27;22(1):859. PMID: 39334374
Phytother Res

Evodiamine alleviates lipopolysaccharide-induced pulmonary inflammation and fibrosis by activating apelin pathway. [Abstract]

2021 Jun;35(6):3406-3417. PMID: 33657655
Am J Chin Med

Evodiamine Suppresses Lung Cancer Progression Through Modulating FAK/STAT3/AKT Signaling Pathway. [Abstract]

2025;53(4):1225-1240. PMID: 40582716
ACS Appl Nano Mater

2024 Mar 8.
J Ethnopharmacol

Mechanisms of pancreatic tumor suppression mediated by Xiang-lian pill: An integrated in silico exploration and experimental validation. [Abstract]

2022 Nov 15:298:115586. PMID: 35931303
Fish Shellfish Immunol

Emodin enhances host antiviral immunity against Micropterus salmoides rhabdovirus by activating RLR signaling in largemouth bass. [Abstract]

2025 Aug 6:166:110633. PMID: 40769268
BMC Complement Med Ther

Network pharmacology-based strategy to investigate the bioactive ingredients and molecular mechanism of Evodia rutaecarpa in colorectal cancer. [Abstract]

2023 Dec 1;23(1):433. PMID: 38041080
Chem Biol Drug Des

Evodiamine inhibits malignant progression of ovarian cancer cells by regulating lncRNA-NEAT1/miR-152-3p/CDK19 axis. [Abstract]

2023 Jul;102(1):101-114. PMID: 36892495

Evodiamine purchased from MedChemExpress. Usage Cited in: Chem Biol Drug Des. 2023 Jul;102(1):101-114. [Abstract]

Evodiamine (EVO; 1.25, 2.5, 5 μM; 48 h) decreased the SKOV3 cell viability in a dose-dependent manner.
Curr Issues Mol Biol

Evodiamine Boosts AR Expression to Trigger Senescence and Halt Proliferation in OSCC Cells. [Abstract]

2025 Jul 17;47(7):558. PMID: 40729027
Pulm Pharmacol Ther

Evodiamine protects against lung ischemia-reperfusion injury by attenuating SYK-associated TLR4/NLRP3 inflammasome activation and ferroptosis-related injury signatures. [Abstract]

2026 Jun:93:102421. PMID: 41951097
J Biochem Mol Toxicol

Evodiamine Enhances Chemosensitivity in Colorectal Cancer by Targeting Ribonucleotide Reductase M2. [Abstract]

2025 Nov;39(11):e70541. PMID: 41121956
Oncol Lett

Evodiamine induces reactive oxygen species-dependent apoptosis and necroptosis in human melanoma A-375 cells. [Abstract]

2020 Oct;20(4):121. PMID: 32863934
Research Square Preprint

2024 Apr 2.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 10 mg/mL (32.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.2964 mL

16.4821 mL

32.9641 mL

5 mM

0.6593 mL

3.2964 mL

6.5928 mL

10 mM

0.3296 mL

1.6482 mL

3.2964 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1 mg/mL (3.30 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline

Solubility: 2.5 mg/mL (8.24 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protokoll

Cell Assay^[1]

Evodiamine is dissolved in DMSO and diluted with appropriate medium before use. The evodiamine-inspired new scaffolds are assayed for growth inhibitory activities toward human cancer cell-lines A549 (lung cancer), MDA-MB-435 (breast cancer) and HCT116 (colon cancer) using the MTT assay. Evodiamine and camptithecin are used as reference drugs^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[2]^[3]

Rats: Twelve healthy male Sprague-Dawley rats are randomly divided into 2 groups: the control group (received oral 10 mg/kg dapoxetine alone) and the combination group (10 mg/kg dapoxetine orally co-administered with 100 mg/kg evodiamine). The plasma concentration of dapoxetine and desmethyl dapoxetine are estimated by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), and different pharmacokinetic parameters are calculated^[2].

Mice: A nude mouse xenograft model is established by using 4–6-week-old male BALB/c nude mice. Mice are dosed daily with 20 mg/kg (10 mL/kg) of evodiamine intragastrically, six mice are dosed intraperitoneally with 10 mg/kg of 5-flurouracil (5-FU) twice a week, and six mice are not treated. The tumor volumes are determined by measuring two dimensions, with tumor volume=length×width×width/2. After 2 or 3 weeks of treatment, mice are sacrificed by cervical dislocation under anesthesia with ether, and the tumor tissues are collected^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (283 KB)
SDS (420 KB)
- English - EN (420 KB)
- Français - FR (420 KB)
- Deutsch - DE (420 KB)
- Norwegian - NO (420 KB)
- Español - ES (420 KB)
- Swedish - SV (420 KB)
- Italian - IT (420 KB)
- Korean - KR (420 KB)
- Portuguese - PT (420 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Wang S, et al. Scaffold Diversity Inspired by the Natural Product Evodiamine: Discovery of Highly Potent and Multitargeting Antitumor Agents. J Med Chem. 2015 Aug 27;58(16):6678-96. [Content Brief]

[2]. Li RF,et al. Effects of Evodiamine on the Pharmacokinetics of Dapoxetine and Its Metabolite Desmethyl Dapoxetine inRats. Pharmacology. 2016;97(1-2):43-7. [Content Brief]

[3]. Shi L, et al. Evodiamine exerts anti-tumor effects against hepatocellular carcinoma through inhibiting β-catenin-mediated angiogenesis. Tumour Biol. 2016 Sep;37(9):12791-12803. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.2964 mL	16.4821 mL	32.9641 mL	82.4103 mL
	5 mM	0.6593 mL	3.2964 mL	6.5928 mL	16.4821 mL
	10 mM	0.3296 mL	1.6482 mL	3.2964 mL	8.2410 mL
	15 mM	0.2198 mL	1.0988 mL	2.1976 mL	5.4940 mL
	20 mM	0.1648 mL	0.8241 mL	1.6482 mL	4.1205 mL
	25 mM	0.1319 mL	0.6593 mL	1.3186 mL	3.2964 mL
	30 mM	0.1099 mL	0.5494 mL	1.0988 mL	2.7470 mL

Keywords

Evodiamine

19 Cited Publications

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Evodiamine

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Publications Citing Use of MedChemExpress (MCE) Evodiamine

Biologische Aktivität

Chemical Information

Publications (19)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Evodiamine

Immunology/Inflammation Compound Library

Natural Product Library

Anti-Cancer Compound Library

Medicine Food Homology Compound Library

Traditional Chinese Medicine Active Compound Library

FDA Approved & Pharmacopeial Drug Library

Alkaloids Library

Anti-Obesity Compound Library

Anti-Cancer Natural Product Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Human Metabolite Library

Bioactive Compound Library Max

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Miao Ethnicity Medicine Compound Library

Tibetan Medicine Compound Library

PBCRBS Traditional Chinese Medicine Compound Library

RO5 Drug-like Natural Product Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Mass Spectrometry Human Metabolite Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Mongolian Medicine Compound Library

Natural Product Diversity Scaffold Library

Tonifying Traditional Chinese Medicine Monomer Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Evodiamine

Biologische Aktivität

Chemical Information

Publications (19)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Evodiamine Related Classifications

HY-N0114 Related Classifications