(±)-Evodiamine
Based on 1 publication(s) in Google Scholar
(±)-Evodiamine, a quinazolinocarboline alkaloid, is a Top1 inhibitor. Evodiamine exhibits anti-inflammatory, antiobesity, and antitumor effects. (±)-Evodiamine inhibits the proliferation of a wide variety of tumor cells by inducing their apoptosis.
For research use only. We do not sell to patients.
- Purity: 99.74%
- CAS No.: 518-18-3
- Formula: C19H17N3O
- Molecular Weight:303.36
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
Publications Citing Use of MedChemExpress (MCE) (±)-Evodiamine
MoreAll Topoisomerase Isoforms
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Biological Activity
|
Top1 |
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
0.98 μM
Compound: 1
|
Antiproliferative activity against human A549 cells after 72 hrs by MTT assay
Antiproliferative activity against human A549 cells after 72 hrs by MTT assay
|
[PMID: 27178387] |
| A549 | IC50 |
1.3 μM
Compound: Fig 1A, Cpd 4
|
Antiproliferative activity against human A549 cells
Antiproliferative activity against human A549 cells
|
[PMID: 38897138] |
| A549 | IC50 |
77.24 μM
Compound: 3; Evo
|
Antiproliferative activity against human A549 cells assessed as inhibition rate incubated for 72 hrs by MTT assay
Antiproliferative activity against human A549 cells assessed as inhibition rate incubated for 72 hrs by MTT assay
|
[PMID: 34844412] |
| Bel-7402 | IC50 |
1.19 μM
Compound: 1
|
Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay
Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay
|
[PMID: 29635169] |
| Bel-7402 | IC50 |
1.23 μM
Compound: 1
|
Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay
Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay
|
[PMID: 27178387] |
| BGC-823 | IC50 |
1.16 μM
Compound: 1
|
Antiproliferative activity against human BGC823 cells after 72 hrs by MTT assay
Antiproliferative activity against human BGC823 cells after 72 hrs by MTT assay
|
[PMID: 27178387] |
| Caco-2 | IC50 |
2.73 μM
Compound: 1
|
Antiproliferative activity against human Caco2 cells after 72 hrs by MTT assay
Antiproliferative activity against human Caco2 cells after 72 hrs by MTT assay
|
[PMID: 29635169] |
| CHO | EC50 |
0.86 μM
Compound: 1
|
Agonist activity at rat TRPV1 expressed in CHO cells assessed as induction of 45Ca2+ uptake measured after 5 mins by scintillation counting method
Agonist activity at rat TRPV1 expressed in CHO cells assessed as induction of 45Ca2+ uptake measured after 5 mins by scintillation counting method
|
[PMID: 27159637] |
| GES1 | IC50 |
>30 μM
Compound: EVO
|
Antiproliferative activity against human GES1 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
Antiproliferative activity against human GES1 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
|
[PMID: 37099835] |
| HCCLM9 | IC50 |
1.63 μM
Compound: 1; Evo
|
Antiproliferative activity against human HCCLM9 cells assessed as reduction in cell growth incubated for 48 hrs by CCK-8 assay
Antiproliferative activity against human HCCLM9 cells assessed as reduction in cell growth incubated for 48 hrs by CCK-8 assay
|
[PMID: 36549115] |
| HCCLM9 | IC50 |
9.27 μM
Compound: EVO
|
Antiproliferative activity against human HCCLM9 cells treated for 72 hrs by MTT assay
Antiproliferative activity against human HCCLM9 cells treated for 72 hrs by MTT assay
|
[PMID: 35639640] |
| HCT-116 | IC50 |
32.67 μM
Compound: 3; Evo
|
Antiproliferative activity against human HCT-116 cells assessed as inhibition rate incubated for 72 hrs by MTT assay
Antiproliferative activity against human HCT-116 cells assessed as inhibition rate incubated for 72 hrs by MTT assay
|
[PMID: 34844412] |
| HEK293 | EC50 |
0.1 μM
Compound: 1
|
Partial antagonist activity at rat TRPV1 expressed in HEK293 cells assessed as capsaicin EC50 for induction of channel current at 0.1 uM at -60 mV holding potential by whole-cell patch clamp method (Rvb = 0.06 uM)
Partial antagonist activity at rat TRPV1 expressed in HEK293 cells assessed as capsaicin EC50 for induction of channel current at 0.1 uM at -60 mV holding potential by whole-cell patch clamp method (Rvb = 0.06 uM)
|
[PMID: 27159637] |
| HEK293 | EC50 |
0.18 μM
Compound: 1
|
Partial antagonist activity at rat TRPV1 expressed in HEK293 cells assessed as capsaicin EC50 for induction of channel current at 0.6 uM at -60 mV holding potential by whole-cell patch clamp method (Rvb = 0.06 uM)
Partial antagonist activity at rat TRPV1 expressed in HEK293 cells assessed as capsaicin EC50 for induction of channel current at 0.6 uM at -60 mV holding potential by whole-cell patch clamp method (Rvb = 0.06 uM)
|
[PMID: 27159637] |
| HEK293 | EC50 |
0.33 μM
Compound: 1
|
Partial antagonist activity at rat TRPV1 expressed in HEK293 cells assessed as capsaicin EC50 for induction of channel current at 1 uM at -60 mV holding potential by whole-cell patch clamp method (Rvb = 0.06 uM)
Partial antagonist activity at rat TRPV1 expressed in HEK293 cells assessed as capsaicin EC50 for induction of channel current at 1 uM at -60 mV holding potential by whole-cell patch clamp method (Rvb = 0.06 uM)
|
[PMID: 27159637] |
| HEK293 | EC50 |
0.6 μM
Compound: 1
|
Agonist activity at rat TRPV1 channel expressed in HEK293 cells assessed as induction of channel current at -60 mV holding potential by whole-cell patch-clamp method
Agonist activity at rat TRPV1 channel expressed in HEK293 cells assessed as induction of channel current at -60 mV holding potential by whole-cell patch-clamp method
|
[PMID: 27159637] |
| HEK293 | EC50 |
2.38 μM
Compound: 1
|
Partial antagonist activity at rat TRPV1 expressed in HEK293 cells assessed as capsaicin EC50 for induction of channel current at 10 uM at -60 mV holding potential by whole-cell patch clamp method (Rvb = 0.06 uM)
Partial antagonist activity at rat TRPV1 expressed in HEK293 cells assessed as capsaicin EC50 for induction of channel current at 10 uM at -60 mV holding potential by whole-cell patch clamp method (Rvb = 0.06 uM)
|
[PMID: 27159637] |
| HEK293 | IC50 |
>100 μM
Compound: 3; Evo
|
Antiproliferative activity against HEK293 cells assessed as inhibition rate incubated for 72 hrs by MTT assay
Antiproliferative activity against HEK293 cells assessed as inhibition rate incubated for 72 hrs by MTT assay
|
[PMID: 34844412] |
| HEK293 | IC50 |
0.44 μM
Compound: 1
|
Partial antagonist activity at rat TRPV1 expressed in HEK293 cells assessed as inhibition of capsaicin-induced inward current at -60 mV holding potential pretreated for 180 secs prior to capsaicin addition in presence of extracellular calcium by whole-cel
Partial antagonist activity at rat TRPV1 expressed in HEK293 cells assessed as inhibition of capsaicin-induced inward current at -60 mV holding potential pretreated for 180 secs prior to capsaicin addition in presence of extracellular calcium by whole-cel
|
[PMID: 27159637] |
| HepG2 | IC50 |
2.67 μM
Compound: EVO
|
Antiproliferative activity against human HepG2 cells treated for 72 hrs by MTT assay
Antiproliferative activity against human HepG2 cells treated for 72 hrs by MTT assay
|
[PMID: 35639640] |
| HepG2 | IC50 |
21.52 μM
Compound: 1; Evo
|
Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by CCK-8 assay
Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by CCK-8 assay
|
[PMID: 36549115] |
| HepG2 | IC50 |
9.32 μM
Compound: 1
|
Antiproliferative activity against human HepG2 cells treated for 24 hrs measured after 72 hrs by MTT assay
Antiproliferative activity against human HepG2 cells treated for 24 hrs measured after 72 hrs by MTT assay
|
[PMID: 29037951] |
| HGC-27 | IC50 |
>30 μM
Compound: EVO
|
Antiproliferative activity against human HGC-27 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
Antiproliferative activity against human HGC-27 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
|
[PMID: 37099835] |
| HGC-27 | IC50 |
2.28 μM
Compound: 1; Evo
|
Antiproliferative activity against human HGC-27 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
Antiproliferative activity against human HGC-27 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
|
[PMID: 36549115] |
| HGC-27 | IC50 |
2.28 μM
Compound: Evo
|
Antiproliferative activity against human HGC-27 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay
Antiproliferative activity against human HGC-27 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay
|
[PMID: 35728506] |
| HL-60 | IC50 |
1.36 μM
Compound: 1
|
Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay
Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay
|
[PMID: 29635169] |
| HL-60 | IC50 |
1.39 μM
Compound: 1
|
Antiproliferative activity against human HL60 cells treated for 24 hrs measured after 72 hrs by MTT assay
Antiproliferative activity against human HL60 cells treated for 24 hrs measured after 72 hrs by MTT assay
|
[PMID: 29037951] |
| Huh-7 | IC50 |
0.78 μM
Compound: 1; Evo
|
Antiproliferative activity against human Huh-7 cells assessed as reduction in cell viability incubated for 48 hrs by CCK-8 assay
Antiproliferative activity against human Huh-7 cells assessed as reduction in cell viability incubated for 48 hrs by CCK-8 assay
|
[PMID: 36549115] |
| Huh-7 | IC50 |
2.18 μM
Compound: EVO
|
Antiproliferative activity against human Huh-7 cells treated for 72 hrs by MTT assay
Antiproliferative activity against human Huh-7 cells treated for 72 hrs by MTT assay
|
[PMID: 35639640] |
| L02 | IC50 |
0.34 μM
Compound: 1
|
Antiproliferative activity against human L02 cells after 72 hrs by MTT assay
Antiproliferative activity against human L02 cells after 72 hrs by MTT assay
|
[PMID: 27178387] |
| L02 | IC50 |
3.95 μM
Compound: EVO
|
Antiproliferative activity against human L02 cells treated for 72 hrs by MTT assay
Antiproliferative activity against human L02 cells treated for 72 hrs by MTT assay
|
[PMID: 35639640] |
| LoVo | IC50 |
24.19 μM
Compound: EVO
|
Antiproliferative activity against human LoVo cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
Antiproliferative activity against human LoVo cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
|
[PMID: 37099835] |
| MCF-10A | IC50 |
>100 μM
Compound: 3; Evo
|
Antiproliferative activity against human MCF-10A cells assessed as inhibition rate incubated for 72 hrs by MTT assay
Antiproliferative activity against human MCF-10A cells assessed as inhibition rate incubated for 72 hrs by MTT assay
|
[PMID: 34844412] |
| MCF7 | IC50 |
0.3 μM
Compound: 48
|
Antiproliferative activity against human MCF7 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay
Antiproliferative activity against human MCF7 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay
|
[PMID: 36375335] |
| MCF7 | IC50 |
28.53 μM
Compound: 1
|
Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay
Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay
|
[PMID: 29635169] |
| MDA-MB-231 | IC50 |
13.3 μM
Compound: 3; Evo
|
Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition rate incubated for 72 hrs by MTT assay
Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition rate incubated for 72 hrs by MTT assay
|
[PMID: 34844412] |
| MDA-MB-435 | IC50 |
6.3 μM
Compound: 3; Evo
|
Antiproliferative activity against human MDA-MB-435 cells assessed as inhibition rate incubated for 72 hrs by MTT assay
Antiproliferative activity against human MDA-MB-435 cells assessed as inhibition rate incubated for 72 hrs by MTT assay
|
[PMID: 34844412] |
| MGC-803 | IC50 |
1.37 μM
Compound: Evo
|
Antiproliferative activity against human MGC-803 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay
Antiproliferative activity against human MGC-803 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay
|
[PMID: 35728506] |
| MGC-803 | IC50 |
12 μM
Compound: Fig 1A, Cpd 4
|
Antiproliferative activity against human MGC-803 cells
Antiproliferative activity against human MGC-803 cells
|
[PMID: 38897138] |
| MGC-803 | IC50 |
8.52 μM
Compound: EVO
|
Antiproliferative activity against human MGC-803 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
Antiproliferative activity against human MGC-803 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
|
[PMID: 37099835] |
| NCI/ADR-RES | IC50 |
0.47 μM
Compound: 48
|
Antiproliferative activity against human MCF7ADR cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay
Antiproliferative activity against human MCF7ADR cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay
|
[PMID: 36375335] |
| NCI-H1650 | IC50 |
4.53 μM
Compound: 48
|
Antiproliferative activity against human NCI-H1650 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay
Antiproliferative activity against human NCI-H1650 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay
|
[PMID: 36375335] |
| NCI-H1975 | IC50 |
5.59 μM
Compound: 48
|
Antiproliferative activity against human NCI-H1975 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay
Antiproliferative activity against human NCI-H1975 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay
|
[PMID: 36375335] |
| PBMC | IC50 |
20.96 μM
Compound: 1
|
Antiproliferative activity against human PBMC cells after 72 hrs by MTT assay
Antiproliferative activity against human PBMC cells after 72 hrs by MTT assay
|
[PMID: 29635169] |
| PBMC | IC50 |
22.87 μM
Compound: 1
|
Antiproliferative activity against human PBMC treated for 24 hrs measured after 72 hrs by MTT assay
Antiproliferative activity against human PBMC treated for 24 hrs measured after 72 hrs by MTT assay
|
[PMID: 29037951] |
| PC-3 | IC50 |
13.97 μM
Compound: 1
|
Antiproliferative activity against human PC3 cells treated for 24 hrs measured after 72 hrs by MTT assay
Antiproliferative activity against human PC3 cells treated for 24 hrs measured after 72 hrs by MTT assay
|
[PMID: 29037951] |
| RKO | IC50 |
29.65 μM
Compound: EVO
|
Antiproliferative activity against human RKO cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
Antiproliferative activity against human RKO cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
|
[PMID: 37099835] |
| SGC-7901 | IC50 |
17.71 μM
Compound: EVO
|
Antiproliferative activity against human SGC-7901 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
Antiproliferative activity against human SGC-7901 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
|
[PMID: 37099835] |
| SGC-7901 | IC50 |
3.6 μM
Compound: Evo
|
Antiproliferative activity against human SGC-7901 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay
Antiproliferative activity against human SGC-7901 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay
|
[PMID: 35728506] |
| SGC-7901 | IC50 |
6.93 μM
Compound: 1
|
Antiproliferative activity against human SGC7901 cells after 72 hrs by MTT assay
Antiproliferative activity against human SGC7901 cells after 72 hrs by MTT assay
|
[PMID: 29635169] |
| SK-HEP1 | IC50 |
2.92 μM
Compound: EVO
|
Antiproliferative activity against human SK-HEP1 cells treated for 72 hrs by MTT assay
Antiproliferative activity against human SK-HEP1 cells treated for 72 hrs by MTT assay
|
[PMID: 35639640] |
| SMMC-7721 | IC50 |
3.04 μM
Compound: EVO
|
Antiproliferative activity against human SMMC-7721 cells treated for 72 hrs by MTT assay
Antiproliferative activity against human SMMC-7721 cells treated for 72 hrs by MTT assay
|
[PMID: 35639640] |
| SW480 | IC50 |
>30 μM
Compound: EVO
|
Antiproliferative activity against human SW480 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
Antiproliferative activity against human SW480 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
|
[PMID: 37099835] |
| SW480 | IC50 |
10 μM
Compound: Fig 1A, Cpd 4
|
Antiproliferative activity against human SW480 cells
Antiproliferative activity against human SW480 cells
|
[PMID: 38897138] |
| THP-1 | IC50 |
3.37 μM
Compound: 1
|
Antiproliferative activity against human THP1 cells treated for 24 hrs measured after 72 hrs by MTT assay
Antiproliferative activity against human THP1 cells treated for 24 hrs measured after 72 hrs by MTT assay
|
[PMID: 29037951] |
Chemical Information
-
CAS No. 518-18-3
-
Appearance Solid
-
Molecular Weight 303.36
-
Formula C19H17N3O
-
Color Off-white to light brown
-
SMILES
O=C1C2=CC=CC=C2N(C)C(N1CC3)C4=C3C5=CC=CC=C5N4
-
Structure Classification
-
Initial Source
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
-
Journal Impact Factor
-
Most Recent
-
J Chromatogr A
Stereoselectivity of evodiamine enantiomers in neuroprotective activity, pharmacokinetics and the ability across the blood-brain barrier. [Abstract]2025 Feb 22:1743:465658. PMID: 39808905
Solvent & Solubility
DMSO : 10 mg/mL (32.96 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2964 mL | 16.4821 mL | 32.9641 mL | 82.4103 mL |
| 5 mM | 0.6593 mL | 3.2964 mL | 6.5928 mL | 16.4821 mL | |
| 10 mM | 0.3296 mL | 1.6482 mL | 3.2964 mL | 8.2410 mL | |
| 15 mM | 0.2198 mL | 1.0988 mL | 2.1976 mL | 5.4940 mL | |
| 20 mM | 0.1648 mL | 0.8241 mL | 1.6482 mL | 4.1205 mL | |
| 25 mM | 0.1319 mL | 0.6593 mL | 1.3186 mL | 3.2964 mL | |
| 30 mM | 0.1099 mL | 0.5494 mL | 1.0988 mL | 2.7470 mL |