CR-8020

CR-8020 (0.0001-100 mg/mL) binds with high affinity to H3N2 HA from the A/Wyoming/3/2003 strain (IC₅₀ 0.06 nM) and H3N2 HA from the A/Brisbane/10/2007 strain (IC₅₀ 3.36 nM), but shows negligible binding to H7N9 HA from the A/Shanghai/02/2013 strain^[1].
Most epitope residues of H3N2 and H7N9 HA targeted by CR-8020 are susceptible to sequence drift and host selection pressure, with multiple predicted single-base escape mutations; among these, the D19N escape mutation is present in a circulating H7N9 strain^[1].
CR-8020 potently neutralizes and binds to group 2 influenza A virus strains by targeting a highly conserved HA epitope^[2].
CR-8020 (1-10000 ng/mL; 6 h) mediates antibody-dependent cell-mediated cytotoxicity (ADCC) in H3N2-infected A549 cells via activation of FcγRIIIa, but its potency is approximately 10-fold lower than that of antibodies targeting the interface of influenza HA trimers^[1].
The ternary complexes HA-CR8020-FcγRIIa and HA-CR8020-FcγRIIIa formed by CR-8020 are subject to steric hindrance from viral or cell membranes, which limits the ability of CR8020 to activate FcγRIIa and FcγRIIIa^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.