Crystal violet, IND (0.0-2.0)
Based on 1 Customer Validation
Crystal violet, IND (0.0-2.0) is a specialized indicator suitable for strongly acidic systems, which reflects changes in pH within the range of 0.0-2.0 through color changes. Crystal violet, IND (0.0-2.0) is widely used in acid-base titration, chemical analysis, and industrial testing fields.
For research use only. We do not sell to patients.
- CAS No.: 548-62-9
- Formula: C25H30ClN3
- Molecular Weight:407.98
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Description
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| H9c2 | CC50 |
1.5 μM
Compound: Gentian violet
|
Cytotoxicity against rat H9c2 cells assessed as reduction in cell viability after 72 hrs by alamar blue assay
Cytotoxicity against rat H9c2 cells assessed as reduction in cell viability after 72 hrs by alamar blue assay
|
[PMID: 29031078] |
| HeLa | IC50 |
0.4 μM
Compound: Gentian violet
|
Antiproliferative activity against human HeLa cells
Antiproliferative activity against human HeLa cells
|
[PMID: 30108998] |
| Huh-7 | CC50 |
1.434 μM
Compound: GNF-Pf-880
|
NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)
NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)
|
[PMID: 18579783] |
| J774 | CC50 |
0.45 μM
Compound: Gentian violet
|
Cytotoxicity against mouse J774 cells assessed as reduction in cell viability after 72 hrs by alamar blue assay
Cytotoxicity against mouse J774 cells assessed as reduction in cell viability after 72 hrs by alamar blue assay
|
[PMID: 29031078] |
| J774 | CC50 |
0.45 μM
Compound: GV
|
Cytotoxicity against mouse J774 cells assessed as cell viability after 72 hrs by Alamar Blue assay
Cytotoxicity against mouse J774 cells assessed as cell viability after 72 hrs by Alamar Blue assay
|
[PMID: 26549870] |
| J774 | CC50 |
0.8 μM
Compound: Gentian violet
|
Cytotoxicity against mouse J774 cells assessed as reduction in cell viability after 72 hrs by Alamar Blue assay
Cytotoxicity against mouse J774 cells assessed as reduction in cell viability after 72 hrs by Alamar Blue assay
|
[PMID: 32364737] |
| J774 | CC50 |
4.1 μM
Compound: GV
|
Cytotoxicity against BALB/c mouse J774 cells assessed as reduction in cell viability after 72 hrs by alamarBlue assay
Cytotoxicity against BALB/c mouse J774 cells assessed as reduction in cell viability after 72 hrs by alamarBlue assay
|
[PMID: 29523468] |
| NIH3T3 | IC50 |
5.3 μM
Compound: Gentian violet
|
Antiproliferative activity against mouse NIH/3T3 cells
Antiproliferative activity against mouse NIH/3T3 cells
|
[PMID: 30108998] |
| RAW264.7 | IC50 |
1.1 μM
Compound: Gentian violet
|
Antiinflammatory activity in mouse RAW264.7 cells assessed as decrease in IFN-gamma and LPS-induced nitric oxide production after 24 hrs by ELISA
Antiinflammatory activity in mouse RAW264.7 cells assessed as decrease in IFN-gamma and LPS-induced nitric oxide production after 24 hrs by ELISA
|
[PMID: 35998343] |
| SK-MEL-2 | IC50 |
1.36 μM
Compound: Crystal Violet
|
Cytotoxicity against human SK-MEL-2 cells expressing NRAS mutant assessed as cell growth inhibition incubated for 48 hrs by MTT assay
Cytotoxicity against human SK-MEL-2 cells expressing NRAS mutant assessed as cell growth inhibition incubated for 48 hrs by MTT assay
|
[PMID: 36961300] |
| SK-MEL-2 | IC50 |
2.89 μM
Compound: Crystal Violet
|
Cytotoxicity against human SK-MEL-2 cells assessed as cell growth inhibition incubated for 24 hrs by MTT assay
Cytotoxicity against human SK-MEL-2 cells assessed as cell growth inhibition incubated for 24 hrs by MTT assay
|
[PMID: 36961300] |
In Vitro
Reference procedures for the determination of cationic surfactants by photometric titration[1]:
1. Take an appropriate amount of cationic surfactant solution into a beaker, add deionized water, 0.5 mL of 1 mM Crystal violet, IND, and 4 mL of pH 2.2 buffer solution, then dilute to 40 mL.
2. Use a 0.1 mM potassium poly(vinyl sulfate) (PVSK) solution as the titrant and add it to the sample solution in successive portions.
3. After each addition, measure the absorbance of the solution at 590 nm using a spectrophotometer.
4. Before titration, the Crystal violet, IND does not react with the cationic surfactant, and the absorbance corresponds to the indicator itself. Before the equivalence point, the absorbance decreases slightly due to dilution by the titrant and ion association.
5. At the end point, the association of the Crystal violet, IND with the titrant causes a sharp decrease in its concentration, leading to a sudden drop in absorbance. The break point of the titration curve is taken as the end point.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 548-62-9
-
Appearance Solid
-
Molecular Weight 407.98
-
Formula C25H30ClN3
-
Color Green to dark green
-
SMILES
C/[N+](C)=C1C=C/C(C=C/1)=C(C2=CC=C(N(C)C)C=C2)\C3=CC=C(N(C)C)C=C3.[Cl-]
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (122.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (273 KB)
-
SDS (922 KB)
- English - EN (922 KB)
- Français - FR (922 KB)
- Deutsch - DE (922 KB)
- Norwegian - NO (922 KB)
- Español - ES (922 KB)
- Swedish - SV (922 KB)
- Italian - IT (922 KB)
- Korean - KR (922 KB)
- Portuguese - PT (922 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4511 mL | 12.2555 mL | 24.5110 mL | 61.2775 mL |
| 5 mM | 0.4902 mL | 2.4511 mL | 4.9022 mL | 12.2555 mL | |
| 10 mM | 0.2451 mL | 1.2256 mL | 2.4511 mL | 6.1278 mL | |
| 15 mM | 0.1634 mL | 0.8170 mL | 1.6341 mL | 4.0852 mL | |
| 20 mM | 0.1226 mL | 0.6128 mL | 1.2256 mL | 3.0639 mL | |
| 25 mM | 0.0980 mL | 0.4902 mL | 0.9804 mL | 2.4511 mL | |
| 30 mM | 0.0817 mL | 0.4085 mL | 0.8170 mL | 2.0426 mL | |
| 40 mM | 0.0613 mL | 0.3064 mL | 0.6128 mL | 1.5319 mL | |
| 50 mM | 0.0490 mL | 0.2451 mL | 0.4902 mL | 1.2256 mL | |
| 60 mM | 0.0409 mL | 0.2043 mL | 0.4085 mL | 1.0213 mL | |
| 80 mM | 0.0306 mL | 0.1532 mL | 0.3064 mL | 0.7660 mL | |
| 100 mM | 0.0245 mL | 0.1226 mL | 0.2451 mL | 0.6128 mL |
Crystal violet, IND (0.0-2.0) Related Classifications
HY-W110794 Related Classifications
Powered by Bioz