DCM-KPV
DCM-KPV is a fluorescent probe targeting the human intestinal oligopeptide transporter PEPT1/SLC15A1 receptor (λex=480 nm, λem=620-670 nm). DCM-KPV specifically binds to PepT1 via its KPV domain and mediates receptor-targeted internalization, thus effectively accumulating in the cytoplasm and nucleus of cells overexpressing this receptor. DCM-KPV has the advantages of long emission wavelength, high emission efficiency, low photobleaching, and negligible cytotoxicity. DCM-KPV maintains stable fluorescence intensity under continuous illumination, exhibiting extremely high live cell compatibility. DCM-KPV can specifically accumulate at colonic inflammatory sites through the intestinal mucosa, enabling direct non-invasive visual differentiation between chronic and acute ulcerative colitis groups and the normal group.
For research use only. We do not sell to patients.
- CAS No.: 2098632-04-1
- Formula: C40H48N8O4
- Molecular Weight:704.86
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
DCM-KPV (1-30 μM; 24-72 h) exhibits negligible cytotoxicity and high cytocompatibility with Caco-2 cells[2].
DCM-KPV (1.0 μM; 50 min) is efficiently internalized into Caco-2 cells via PepT1 receptor-mediated uptake, accumulating in the cytoplasm and nucleus[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:PepT1-overexpressed Caco-2 human colon adenocarcinoma cells
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Concentration:1-30 μM (viability assays); 1-30 μM (apoptosis assays)
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Incubation Time:24-72 h (viability assays); 24 h (apoptosis assays)
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Result:Maintained >95% cell viability at all tested concentrations and incubation times.
Showed no significant differences in cell proliferation rate or cell growth inhibition rate relative to controls after 24, 48, and 72 h of incubation.
Showed no appreciable increase in apoptosis rate relative to controls after 24 h of incubation.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c mice with Ulcerative colitis (male, 6-8-week-old)[2]
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Dosage:2.5 mg/kg
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Administration:via anus; single dose; reserved for 1 hour
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Result:Reached an average fluorescence intensity of 9.33E+08 a.u. in distal colon tissues of chronic colitis mice.
Reached an average fluorescence intensity of 4.08E+08 a.u. in distal colon tissues of acute colitis mice.
Reached an average fluorescence intensity of 2.25E+08 a.u. in distal colon tissues of normal control mice.
Chemical Information
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CAS No. 2098632-04-1
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Molecular Weight 704.86
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Formula C40H48N8O4
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SMILES
CC(C(C(N1CCN(CC1)C2=CC=C(C=C2)/C=C/C3=C/C(C4=CC=CC=C4O3)=C(C#N)/C#N)=O)NC(C5CCCN5C(C(CCCCN)N)=O)=O)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
DCM-KPV Related Classifications
HY-D3210 Related Classifications
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