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  4. DCM-KPV

DCM-KPV is a fluorescent probe targeting the human intestinal oligopeptide transporter PEPT1/SLC15A1 receptor (λex=480 nm, λem=620-670 nm). DCM-KPV specifically binds to PepT1 via its KPV domain and mediates receptor-targeted internalization, thus effectively accumulating in the cytoplasm and nucleus of cells overexpressing this receptor. DCM-KPV has the advantages of long emission wavelength, high emission efficiency, low photobleaching, and negligible cytotoxicity. DCM-KPV maintains stable fluorescence intensity under continuous illumination, exhibiting extremely high live cell compatibility. DCM-KPV can specifically accumulate at colonic inflammatory sites through the intestinal mucosa, enabling direct non-invasive visual differentiation between chronic and acute ulcerative colitis groups and the normal group.

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DCM-KPV

DCM-KPV Chemical Structure

CAS No. : 2098632-04-1

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DCM-KPV Related Antibodies

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Description

DCM-KPV is a fluorescent probe targeting the human intestinal oligopeptide transporter PEPT1/SLC15A1 receptor (λex=480 nm, λem=620-670 nm). DCM-KPV specifically binds to PepT1 via its KPV domain and mediates receptor-targeted internalization, thus effectively accumulating in the cytoplasm and nucleus of cells overexpressing this receptor. DCM-KPV has the advantages of long emission wavelength, high emission efficiency, low photobleaching, and negligible cytotoxicity. DCM-KPV maintains stable fluorescence intensity under continuous illumination, exhibiting extremely high live cell compatibility. DCM-KPV can specifically accumulate at colonic inflammatory sites through the intestinal mucosa, enabling direct non-invasive visual differentiation between chronic and acute ulcerative colitis groups and the normal group[1][2].

In Vitro

DCM-KPV (1-30 μM; 24-72 h) exhibits negligible cytotoxicity and high cytocompatibility with Caco-2 cells[2].
DCM-KPV (1.0 μM; 50 min) is efficiently internalized into Caco-2 cells via PepT1 receptor-mediated uptake, accumulating in the cytoplasm and nucleus[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DCM-KPV Related Antibodies

Cell Cytotoxicity Assay[2]

Cell Line: PepT1-overexpressed Caco-2 human colon adenocarcinoma cells
Concentration: 1-30 μM (viability assays); 1-30 μM (apoptosis assays)
Incubation Time: 24-72 h (viability assays); 24 h (apoptosis assays)
Result: Maintained >95% cell viability at all tested concentrations and incubation times.
Showed no significant differences in cell proliferation rate or cell growth inhibition rate relative to controls after 24, 48, and 72 h of incubation.
Showed no appreciable increase in apoptosis rate relative to controls after 24 h of incubation.
In Vivo

DCM-KPV (2.5 mg/kg; via anus; single dose; reserved for 1 hour) achieves noninvasive visualization of ulcerative colitis in Mus musculus (mice), with significantly higher fluorescence accumulation in chronic colitis than acute colitis and normal colon tissues, enabling discrimination between chronic, acute, and normal groups[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c mice with Ulcerative colitis (male, 6-8-week-old)[2]
Dosage: 2.5 mg/kg
Administration: via anus; single dose; reserved for 1 hour
Result: Reached an average fluorescence intensity of 9.33E+08 a.u. in distal colon tissues of chronic colitis mice.
Reached an average fluorescence intensity of 4.08E+08 a.u. in distal colon tissues of acute colitis mice.
Reached an average fluorescence intensity of 2.25E+08 a.u. in distal colon tissues of normal control mice.
Molecular Weight

704.86

Formula

C40H48N8O4
CAS No.
SMILES

CC(C(C(N1CCN(CC1)C2=CC=C(C=C2)/C=C/C3=C/C(C4=CC=CC=C4O3)=C(C#N)/C#N)=O)NC(C5CCCN5C(C(CCCCN)N)=O)=O)C
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Room temperature in continental US; may vary elsewhere.
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Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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DCM-KPV Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DCM-KPV2098632-04-1Fluorescent Dyecytoplasmulcerative colitisnucleusPepT1 receptorcolon inflammation regionsintestinal mucosaCaco-2 cellsPepT1-overexpressing cellsmiceInhibitorinhibitorinhibit

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