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  3. Dehydroabietic acid

Dehydroabietic acid is a diterpene resin acid that can be isolated from Pinus and Picea. Dehydroabietic acid has anti-bacterial, anti-fungal, anti-inflammatory, and anticancer activities. Dehydroabietic acid is a dual PPAR-α/γ agonist and PPAR-γ partial agonist, which can attenuate insulin resistance (IR) and hepatic steatosis induced by HFD-consumption in mice.

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Dehydroabietic acid Chemical Structure

Dehydroabietic acid Chemical Structure

CAS No. : 1740-19-8

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 77 In-stock
Solution
10 mM * 1 mL in DMSO USD 77 In-stock
Solid
5 mg USD 70 In-stock
10 mg USD 120 In-stock
20 mg USD 200 In-stock
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Based on 1 publication(s) in Google Scholar

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  • Biological Activity

  • Purity & Documentation

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Description

Dehydroabietic acid is a diterpene resin acid that can be isolated from Pinus and Picea. Dehydroabietic acid has anti-bacterial, anti-fungal, anti-inflammatory, and anticancer activities. Dehydroabietic acid is a dual PPAR-α/γ agonist and PPAR-γ partial agonist, which can attenuate insulin resistance (IR) and hepatic steatosis induced by HFD-consumption in mice[1][2].

In Vitro

Dehydroabietic acid (0-100 μM, 30 min) decreases NO production in RAW264.7 cells[1].
Dehydroabietic acid (0-100 μM, 6 h) reduces the mRNA expression levels of inflammatory mediators including inducible nitric oxide (iNOS) and TNF-α in RAW264.7 cells[1].
Dehydroabietic acid (0-100 μM, 24 h) reduces the MyD88-induced NF-κB and AP-1 transcriptional activities in HEK293T cells[1].
Dehydroabietic acid (100 μM, 24 h) inactivates both Src and Syk kinases in Src- or Syk-overexpressing HEK293T cells[1].
Dehydroabietic acid (2.5-15 μM, 4 days) promotes 3T3-L1 adipocyte differentiation in a dose-dependent manner[2].
Dehydroabietic acid (10 μM, 0-6 days) increases mRNA expression of PPAR-γ target genes (Glut-4 and Cyp4a10) in 3T3-L1 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: AW264.7 cells
Concentration: 100 μM
Incubation Time: 5-60 min, 120-240 min
Result: Blocked the phosphorylation of IκBα at 5 and 15 min.
Reduced c-Jun N-terminal kinase (JNK) phosphorylation.
Reduced phosphorylated levels of mitogen-activated protein kinase kinase 4 (MKK4) and MKK7 at 60 mim.
Decreased the phosphorylation of MKK4 and MKK7 and their downstream protein JNK at 120 and 240 min.
In Vivo

Dehydroabietic acid (10-20 mg/kg, i.g., daily, 9 weeks) alleviates HFD-induced hepatic steatosis and inflammation in HFD mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: HFD mice[2]
Dosage: 10-20 mg/kg
Administration: i.g., daily, 9 weeks
Result: Decreased the levels of liver injury markers, ALT and AST.
Decreased blood TG, TC and LDL-c levels and increased the HDL-c levels.
Activated PPAR-α and its target gene which were reduced by HFD, including acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain and CPT1α.
Decreased mRNA expression of inflammatory factors commonly involved in liver diseases and injury (IL-1β, IL-6, TNF-α, COX-1 and COX-2).
Upregulated mRNA expression of PPAR-γ, Glut-4, Adipor, FSP27, ACOX-1, FABP4, Adiponectin.
Molecular Weight

300.44

Formula

C20H28O2

CAS No.
Appearance

Solid

Color

White to yellow

SMILES

O=C([C@]1(C)CCC[C@]2(C)C3=C(CC[C@@]12[H])C=C(C(C)C)C=C3)O

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (332.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3285 mL 16.6423 mL 33.2845 mL
5 mM 0.6657 mL 3.3285 mL 6.6569 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (8.32 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (8.32 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
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Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.75%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3285 mL 16.6423 mL 33.2845 mL 83.2113 mL
5 mM 0.6657 mL 3.3285 mL 6.6569 mL 16.6423 mL
10 mM 0.3328 mL 1.6642 mL 3.3285 mL 8.3211 mL
15 mM 0.2219 mL 1.1095 mL 2.2190 mL 5.5474 mL
20 mM 0.1664 mL 0.8321 mL 1.6642 mL 4.1606 mL
25 mM 0.1331 mL 0.6657 mL 1.3314 mL 3.3285 mL
30 mM 0.1109 mL 0.5547 mL 1.1095 mL 2.7737 mL
40 mM 0.0832 mL 0.4161 mL 0.8321 mL 2.0803 mL
50 mM 0.0666 mL 0.3328 mL 0.6657 mL 1.6642 mL
60 mM 0.0555 mL 0.2774 mL 0.5547 mL 1.3869 mL
80 mM 0.0416 mL 0.2080 mL 0.4161 mL 1.0401 mL
100 mM 0.0333 mL 0.1664 mL 0.3328 mL 0.8321 mL
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Dehydroabietic acid
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HY-N6869
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