- Fluorescent Dyes
- Fluorescence Wavelength ranges
- Excitation Wavelength (Ex)
- Ex 571-590 nm Yellow
Ex 571-590 nm Yellow
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Ex 571-590 nm Yellow (18)
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- Formula: C20H24BF2N5O
- Molecular Weight: 399.25
LysoTracker Red is a Red fluorescently labeled lysosomal probe with a maximum excitation/emission wavelength of 577/590 nm. The structure is composed of a fluorescein group and linked weak bases, which can freely cross the cell membrane and gather on spherical organelles. It is suitable for observing the internal biosynthesis and related pathogenesis of lysosomes.
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- Formula: C44H42BClF2N6O7S2
- Molecular Weight: 915.23
ER-Tracker dye is a derivative of BODIPY series dyes coupled with Glibenclamide (HY-15206), highly selective binding to the endoplasmic reticulum, non-toxic to cells at low concentrations, this type of dye is an environmentally sensitive probe, and formaldehyde treatment can still retain part of the fluorescence, with high fluorescence life, good extinction coefficient and other characteristics. Glibenclamide is an atp-dependent K+ channel blocker (Kir6, KATP) and CFTR Cl-channel blocker that binds in the endoplasmic reticulum. ER-Tracker is not suitable for staining cells after fixation. Ex/Em=587/615 nm.
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- Formula: C31H30N2O7S2
- Molecular Weight: 606.71
Texas Red (Sulforhodamine 101) is an amphoteric rhodamine red fluorescent dye (excitation/emission: 586/605 nm). Texas Red is used extensively for investigating neuronal morphology and acts as acell type-selective fluorescent marker of astrocytes bothin vivoand in slice preparations.
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- Formula: C42H40ClN3O4
- Molecular Weight: 686.24
CellTracker Red CMTPX is a cell-permeable fluorescent dye that can be used as a cell tracer for monitoring cell movement and location (Ex/Em=586/614 nm).
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- Formula: C14H11NO4
- Molecular Weight: 257.24
ADHP is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm).
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- Formula: C39H50BF2N3O4S
- Molecular Weight: 705.70
The Golgi apparatus is composed of flattened vesicles superimposed on each other by unit membranes. The flattened vesicles are round with expanded and perforated edges. The Golgi fluorescent probe is a BODIPY-labeled ceramide derivative, the synthesis of which occurs in the endoplasmic reticulum and can then be transported to the Golgi via ceramide transport protein (CERT) or vesicular translocation, allowing specific labeling of the dye. BODIPY TR Ceramide (Golgi-Red Tracke) is a Golgi-specific fluorescent dye, which can visualise individual cells. Ex/Em=589 nm/616 nm.
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- Formula: C26H28BClF2N4O2S
- Molecular Weight: 544.85
BODIPY TR Cadaverine, a cadaverine derivative, is a red fluorescent dye. BODIPY TR Cadaverine can be used in a a highly sensitive and robust fluorescent displacement assay, which binds to native LPS strongly, specifically recognizing lipid A, and is competitively displaced by compounds displaying an affinity for lipid A.
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- Formula: C12H7NO3
- Molecular Weight: 213.19
Resorufin (NSC 12097) is a highly fluorescent pink dye. Resorufin shows favorable properties such as water solubility, red-shifted absorption/emission signals. Resorufin is used for the detection of ROS/RNS and a second analyte.
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- Formula: C12H6NNaO3
- Molecular Weight: 235.17
Resorufin sodium salt (NSC 12097) is a highly fluorescent pink dye. Resorufin sodium salt shows favorable properties such as water solubility, red-shifted absorption/emission signals. Resorufin sodium salt is used for the detection of ROS/RNS and a second analyte.
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- Formula: C16H14BF2N3O2
- Molecular Weight: 329.11
BODIPY 576/589 is a long wavelength biological labeled dye contains a free COOH-group (Em/Ex = 589/576 nm).
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- Formula: C26H22BF2N3O4
- Molecular Weight: 489.28
BODIPY-581/591 NHS ester is a bright, red fluorescent dye (excitation: 581 nm; emission: 591 nm). BODIPY-581/591 NHS ester can be used for the labeling of amine containing biomolecules.
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- Formula: C33H30N2O5
- Molecular Weight: 534.60
5-ROX (5-Carboxy-X-rhodamine), a rhodamine dye, exhibits strong fluorescence property in aqueous buffer with the λexit of 580 nm (ε=3.6×104 M-1 cm-1), and λemit of 604 nm (=0.94).
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- Formula: C33H30N2O5
- Molecular Weight: 534.60
5(6)-ROX (5(6)-Carboxy-X-rhodamine) is a nucleic acid fluorescent label which can be used as a reference dye for real-time polymerase chain reaction (Em/Ex = 605/585 nm).
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- Formula: C13H9NO3
- Molecular Weight: 227.22
Resorufin methyl ether (Methoxyresorufin) is a cytochrome P450 fluorometric substrate. Resorufin methyl ether is a relatively specific substrate for CYP1A2 activity in rodents.
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- Formula: C33H20O9
- Molecular Weight: 560.51
5(6)-Carboxynaphthofluorescein diacetate is a fluorescent substrate for esterase assays that can be cleaved by intracellular esterases, producing red fluorescence to measure enzyme activity (Ex = 590 nm; Em = 645 nm).
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- Formula: C26H30N2O7S
- Molecular Weight: 514.59
Fluorescent Red Mega 480 is a dye that is specifically designed for multicolor techniques. Fluorescent Red Mega 480 is characterized by an extremely large stoke′s shift between excitation and emission maxima.
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