2. Metabolic Enzyme/Protease MAPK/ERK Pathway PI3K/Akt/mTOR NF-κB
  3. Endogenous Metabolite p38 MAPK Akt Keap1-Nrf2 NF-κB
  4. Ergothioneine

Ergothioneine  (Synonyms: L-(+)-Ergothioneine)

Cat. No.: HY-N1914 Purity: 99.98%
COA
SDS
Handling Instructions Technical Support

Ergothioneine is an imidazole-2-thione derivative with orally active histidine betaine. Ergothioneine is a specific inhibitor of p38-MAPK and Akt, which plays a protective role in cell apoptosis induced by stress. Ergothioneine has antioxidant activity.

For research use only. We do not sell to patients.

CAS No. : 497-30-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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Customer Review

Based on 13 publication(s) in Google Scholar

Other Forms of Ergothioneine:

Ergothioneine Related Antibodies

Top Publications Citing Use of Products

    Ergothioneine purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2024 Jun 12;16(23):29917-29929.  [Abstract]

    Ergothioneine (EGT; 40 μg/mL; 48h). Fluorescence images of live/dead staining.

    Ergothioneine purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2024 Jun 12;16(23):29917-29929.  [Abstract]

    Ergothioneine (EGT; 40 μg/mL; 0-6 days). Survival fraction at different radiation dosages by colony formation assay.

    Ergothioneine purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2024 Jun 12;16(23):29917-29929.  [Abstract]

    Ergothioneine (EGT; 40 μg/mL; 24 h). Analysis of DCF fluorescence by flow cytometry.

    Ergothioneine purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2024 Jun 12;16(23):29917-29929.  [Abstract]

    Ergothioneine (EGT; 40 μg/mL; 24 h). Statistics of apoptosis rate.

    Ergothioneine purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2024 Jun 12;16(23):29917-29929.  [Abstract]

    Ergothioneine (EGT; 40 μg/mL; 24 h). Expression of Bcl-2, Bax, c-Caspase-3, and c-PARP following varying treatments.

    View All Endogenous Metabolite Isoform Specific Products:

    View All Isoforms
    Human Endogenous Metabolite Microbial Metabolite

    View All p38 MAPK Isoform Specific Products:

    View All Isoforms
    p38 MAPK Akt1 p38α p38β MAPK13 p38δ p38γ

    View All Akt Isoform Specific Products:

    View All Isoforms
    Akt Akt1 Akt2 Akt3

    View All NF-κB Isoform Specific Products:

    View All Isoforms
    NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Ergothioneine is an imidazole-2-thione derivative with orally active histidine betaine. Ergothioneine is a specific inhibitor of p38-MAPK and Akt, which plays a protective role in cell apoptosis induced by stress. Ergothioneine has antioxidant activity[1][2].

    IC50 & Target

    Microbial Metabolite

     

    Human Endogenous Metabolite

     

    Cellular Effect
    Cell Line Type Value Description References
    HEK293 EC50
    40 μM
    Compound: 5351619
    Substrate uptake by the Organic Cation/Carnitine Transporter 1 (OCTN1, SLC22A4) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 cells stably transfected with SLC22A4 (PubChem AID: 1794816)
    Substrate uptake by the Organic Cation/Carnitine Transporter 1 (OCTN1, SLC22A4) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 cells stably transfected with SLC22A4 (PubChem AID: 1794816)
    		10.5281/zenodo.7360363
    In Vitro

    Ergothioneine (0.25, 1 mM, 23 h) can regulate PC12 DNA damage, MAPKs activation and cell death induced by hydrogen peroxide[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Ergothioneine Related Antibodies

    Cell Viability Assay [1]

    Cell Line: PC12
    Concentration: 0.25, 1 mM
    Incubation Time: 23 h
    Result: Increased cell viability by approximately 13% and 34%, respectively.

    Western Blot Analysis [1]

    Cell Line: PC12
    Concentration: 0.25, 1 mM
    Incubation Time: 23 h
    Result: Counteracted the p38 phosphorylation induced by 1 h incubation with H2O2.
    In Vivo

    Ergothioneine (70 mg/kg orally, For 14 consecutive days) mediates the improvement of cisplatin-induced nephrotoxicity in rats by regulating Nrf2, p53 and NF-κB signaling and inhibiting γ-glutamyltranspeptidase[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Cisplatin-treated rats[1]
    Dosage: 70 mg/kg
    Administration: p.o. For 14 consecutive days
    Result: Decreased serum creatinine and BUN.
    Increased GFR at two-fold.
    Suppressed cisplatin-induced oxidative stress and GCT.
    Up-regulated the levels of Nrf2, NQO1, and HO-1.
    Suppresses NF-κB signaling and the pro-inflammatory cytokines.
    Clinical Trial
    Molecular Weight

    229.30

    Formula

    C9H15N3O2S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    S=C1NC(C[C@@H](C([O-])=O)[N+](C)(C)C)=CN1
    Structure Classification
    Initial Source

    many bacteria and most fungi
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 125 mg/mL (545.14 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.3611 mL 21.8055 mL 43.6110 mL
    5 mM 0.8722 mL 4.3611 mL 8.7222 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (436.11 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.98%

    SDS (393 KB)

    COA (249 KB) HNMR (248 KB) RP-HPLC (255 KB) MS (205 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 4.3611 mL 21.8055 mL 43.6110 mL 109.0275 mL
    5 mM 0.8722 mL 4.3611 mL 8.7222 mL 21.8055 mL
    10 mM 0.4361 mL 2.1805 mL 4.3611 mL 10.9027 mL
    15 mM 0.2907 mL 1.4537 mL 2.9074 mL 7.2685 mL
    20 mM 0.2181 mL 1.0903 mL 2.1805 mL 5.4514 mL
    25 mM 0.1744 mL 0.8722 mL 1.7444 mL 4.3611 mL
    30 mM 0.1454 mL 0.7268 mL 1.4537 mL 3.6342 mL
    40 mM 0.1090 mL 0.5451 mL 1.0903 mL 2.7257 mL
    50 mM 0.0872 mL 0.4361 mL 0.8722 mL 2.1805 mL
    60 mM 0.0727 mL 0.3634 mL 0.7268 mL 1.8171 mL
    80 mM 0.0545 mL 0.2726 mL 0.5451 mL 1.3628 mL
    100 mM 0.0436 mL 0.2181 mL 0.4361 mL 1.0903 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Ergothioneine Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Ergothioneine497-30-3L-(+)-ErgothioneineEndogenous Metabolitep38 MAPKAktKeap1-Nrf2NF-κBPKBProtein kinase BNuclear factor-κBNuclear factor-kappaBMAPKAntioxidantPC12CisplatinDNA fragmentationInhibitorinhibitorinhibit

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