Cat. No.: HY-N1914

Ergothioneine

(Synonyms: L-(+)-Ergothioneine)

13 Cited Publications

Customer Review

Based on 13 publication(s) in Google Scholar

Ergothioneine is an imidazole-2-thione derivative with orally active histidine betaine. Ergothioneine is a specific inhibitor of p38-MAPK and Akt, which plays a protective role in cell apoptosis induced by stress. Ergothioneine has antioxidant activity.

For research use only. We do not sell to patients.

Purity: 99.98%
CAS No.: 497-30-3
Formula: C₉H₁₅N₃O₂S
Molecular Weight:229.30

Storage:

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Ergothioneine Chemical Structure

CAS No.: 497-30-3

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (13)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

All p38 MAPK Isoforms :

All Akt Isoforms :

All NF-κB Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	40 μM Compound: 5351619	Substrate uptake by the Organic Cation/Carnitine Transporter 1 (OCTN1, SLC22A4) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 cells stably transfected with SLC22A4 Substrate uptake by the Organic Cation/Carnitine Transporter 1 (OCTN1, SLC22A4) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 cells stably transfected with SLC22A4	10.5281/zenodo.7360363
HEK293	EC₅₀	40 μM Compound: 5351619	Substrate uptake by the Organic Cation/Carnitine Transporter 1 (OCTN1, SLC22A4) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 cells stably transfected with SLC22A4 (PubChem AID: 1794816) Substrate uptake by the Organic Cation/Carnitine Transporter 1 (OCTN1, SLC22A4) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 cells stably transfected with SLC22A4 (PubChem AID: 1794816)	10.5281/zenodo.7360363

In Vitro

Ergothioneine (0.25, 1 mM, 23 h) can regulate PC12 DNA damage, MAPKs activation and cell death induced by hydrogen peroxide^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay ^[1]

Cell Line:

PC12
Concentration:

0.25, 1 mM
Incubation Time:

23 h
Result:

Increased cell viability by approximately 13% and 34%, respectively.

Western Blot Analysis ^[1]

Cell Line:

PC12
Concentration:

0.25, 1 mM
Incubation Time:

23 h
Result:

Counteracted the p38 phosphorylation induced by 1 h incubation with H2O2.

In Vivo

Ergothioneine (70 mg/kg orally, For 14 consecutive days) mediates the improvement of cisplatin-induced nephrotoxicity in rats by regulating Nrf2, p53 and NF-κB signaling and inhibiting γ-glutamyltranspeptidase^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Cisplatin-treated rats^[1]
Dosage:

70 mg/kg
Administration:

p.o. For 14 consecutive days
Result:

Decreased serum creatinine and BUN.
Increased GFR at two-fold.
Suppressed cisplatin-induced oxidative stress and GCT.
Up-regulated the levels of Nrf2, NQO1, and HO-1.
Suppresses NF-κB signaling and the pro-inflammatory cytokines.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 497-30-3
Appearance Solid
Molecular Weight 229.30
Formula C₉H₁₅N₃O₂S
Color White to off-white
SMILES

S=C1NC(C[C@@H](C([O-])=O)[N+](C)(C)C)=CN1
Synonyms

L-(+)-Ergothioneine
Structure Classification
- Alkaloids
- Other Alkaloids
Initial Source
- Microorganisms
- Endogenous metabolite
many bacteria and most fungi
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (13)

All Cell Imaging/StainingCell Proliferation/Viability AssayFlow CytometryApoptosis AnalysisWB

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Journal Impact Factor

Redox Biol

Ergothioneine rescues obesity-induced testicular dysfunction via dual restoration of steroidogenesis and mitochondrial redox homeostasis. [Abstract]

2026 Apr:91:104090. PMID: 41719756
Mol Biomed

Ergothioneine attenuates whole-abdominal irradiation-induced multi-organ injury via the gut-heart-brain axis by modulating calcium voltage-gated channel subunit alpha1 C (Cacna1c) expression. [Abstract]

2026 Jan 14;7(1):6. PMID: 41530565
ACS Appl Mater Interfaces

Ergothioneine-Sodium Hyaluronate Dressing: A Promising Approach for Protecting against Radiation-Induced Skin Injury. [Abstract]

2024 Jun 12;16(23):29917-29929. PMID: 38813785

Ergothioneine purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2024 Jun 12;16(23):29917-29929. [Abstract]

Ergothioneine (EGT; 40 μg/mL; 48h). Fluorescence images of live/dead staining.

Ergothioneine purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2024 Jun 12;16(23):29917-29929. [Abstract]

Ergothioneine (EGT; 40 μg/mL; 0-6 days). Survival fraction at different radiation dosages by colony formation assay.

Ergothioneine purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2024 Jun 12;16(23):29917-29929. [Abstract]

Ergothioneine (EGT; 40 μg/mL; 24 h). Analysis of DCF fluorescence by flow cytometry.

Ergothioneine purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2024 Jun 12;16(23):29917-29929. [Abstract]

Ergothioneine (EGT; 40 μg/mL; 24 h). Statistics of apoptosis rate.

Ergothioneine purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2024 Jun 12;16(23):29917-29929. [Abstract]

Ergothioneine (EGT; 40 μg/mL; 24 h). Expression of Bcl-2, Bax, c-Caspase-3, and c-PARP following varying treatments.
ACS Appl Mater Interfaces

Adhesive Ergothioneine Hyaluronate Gel Protects against Radiation Gastroenteritis by Alleviating Apoptosis, Inflammation, and Gut Microbiota Dysbiosis. [Abstract]

2023 Apr 26;15(16):19833-19846. PMID: 37052616
ACS Environ Au

Machine Learning-Assisted Recognition of Environmental Sulfur-Containing Chemicals in Nontargeted Mass Spectrometry Analysis of Inadequate Mass Resolution. [Abstract]

2025 Aug 5;5(6):573-582. PMID: 41277996
Anal Chem

Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]

2025 Jun 3;97(21):11099-11109. PMID: 40401576
Antioxid Redox Signal

The antioxidant ergothioneine alleviates cisplatin-induced hearing loss through the Nrf2 pathway. [Abstract]

2024 May 21. PMID: 38770822
Int Immunopharmacol

Ergothioneine inhibits the progression of osteoarthritis via the Sirt6/NF-κB axis both in vitro and in vivo. [Abstract]

2023 Jun:119:110211. PMID: 37156032
Sci Rep

Ergothioneine promotes osteogenesis and angiogenesis through PI3K/AKT pathway and prevents glucocorticoid-induced osteonecrosis of the femoral head. [Abstract]

2026 Feb 28;16(1):11411. PMID: 41764247
Hum Exp Toxicol

Anti-EMT properties of ergothioneine attenuate lipopolysaccharide-induced oxidative stress-mediated acute lung injury via modulating TGF-β/smad/snail signaling pathway. [Abstract]

2023 Jan-Dec:42:9603271231178015. PMID: 37212426
SSRN

2025 Nov 17.
bioRxiv

2025 June 02.
bioRxiv

2025 April 08.

Solvent & Solubility

In Vitro:

H₂O : 125 mg/mL (545.14 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.3611 mL

21.8055 mL

43.6110 mL

5 mM

0.8722 mL

4.3611 mL

8.7222 mL

10 mM

0.4361 mL

2.1805 mL

4.3611 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (436.11 mM); Clear solution; Need ultrasonic

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Colognato R, et al. Modulation of hydrogen peroxide-induced DNA damage, MAPKs activation and cell death in PC12 by ergothioneine. Clin Nutr. 2006 Feb;25(1):135-45. [Content Brief]

[2]. Salama SA, et al. Ergothioneine mitigates cisplatin-evoked nephrotoxicity via targeting Nrf2, NF-κB, and apoptotic signaling and inhibiting γ-glutamyl transpeptidase. Life Sci. 2021 Aug 1;278:119572. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	4.3611 mL	21.8055 mL	43.6110 mL	109.0275 mL
	5 mM	0.8722 mL	4.3611 mL	8.7222 mL	21.8055 mL
	10 mM	0.4361 mL	2.1805 mL	4.3611 mL	10.9027 mL
	15 mM	0.2907 mL	1.4537 mL	2.9074 mL	7.2685 mL
	20 mM	0.2181 mL	1.0903 mL	2.1805 mL	5.4514 mL
	25 mM	0.1744 mL	0.8722 mL	1.7444 mL	4.3611 mL
	30 mM	0.1454 mL	0.7268 mL	1.4537 mL	3.6342 mL
	40 mM	0.1090 mL	0.5451 mL	1.0903 mL	2.7257 mL
	50 mM	0.0872 mL	0.4361 mL	0.8722 mL	2.1805 mL
	60 mM	0.0727 mL	0.3634 mL	0.7268 mL	1.8171 mL
	80 mM	0.0545 mL	0.2726 mL	0.5451 mL	1.3628 mL
	100 mM	0.0436 mL	0.2181 mL	0.4361 mL	1.0903 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Ergothioneine

13 Cited Publications

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Ergothioneine

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Publications Citing Use of MedChemExpress (MCE) Ergothioneine