5-PT TFA
Based on 1 publication(s) in Google Scholar
5-PT (5-Propargyltryptamide) TFA is an alkyne-functionalized derivative of 5-HT (HY-B1473A) and serotonylation substrate that can be taken up by living cells. After conjugation with biotin, 5-PT TFA enables the identification of serotonylated proteins via copper click chemistry and mass spectrometry. 5-PT TFA can label and isolate serotonylated cells in alveolar epithelial cells for proteomic analysis. 5-PT TFA is applicable to research related to bronchopulmonary dysplasia.
For research use only. We do not sell to patients.
- CAS No.: 3117303-22-4
- Formula: C15H15F3N2O3
- Molecular Weight:328.29
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) 5-PT TFA
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Biological Activity
Description
In Vitro
5-PT (100 μM; 24 h) TFA labels 121 unique serotonylated proteins in MLE-12 alveolar epithelial cells, with many of these proteins linked to cellular processes and cell behavior regulation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 3117303-22-4
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Molecular Weight 328.29
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Formula C15H15F3N2O3
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SMILES
O=C(C(F)(F)F)O.C#CCOC1=CC2=C(NC=C2CCN)C=C1
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Synonyms
5-Propargyltryptamide TFA
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (1)
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Journal Impact Factor
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Most Recent
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iScience
2025 Nov 19;28(12):114120. PMID: 41438086
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
5-PT TFA Related Classifications
HY-156562B Related Classifications
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