Cinnabarin
Cinnabarin is a natural phenoxazinone red pigment derived from Pycnoporus sanguineus. Cinnabarin reduces rabies virus infection levels in neuroblastoma cells and causes cytopathic effects on neuroblastoma cell monolayers at high concentrations. Cinnabarin exhibits antibacterial activity against bacteria, with a preference for Gram-positive bacteria and human isolates. Cinnabarin can be used in studies related to rabies and drug-resistant Gram-positive bacteria.
For research use only. We do not sell to patients.
- CAS No.: 146-90-7
- Formula: C14H10N2O5
- Molecular Weight:286.24
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
All Endogenous Metabolite Isoforms
More
Biological Activity
Description
In Vitro
Cinnabarin (0.155-5.0 mg/mL; 24 h) exhibits no cytotoxic effects on mouse neuroblastoma NA cells at 0.31 mg/mL or lower, but causes dose-dependent cytopathic effects at concentrations of 0.62 mg/mL and higher, affecting over 50% of cells at 2.5 mg/mL and above[1].
Cinnabarin (0.155-5.0 mg/mL; 24 h) reduces rabies virus infection in mouse neuroblastoma NA cells in a dose-dependent manner, achieving a four-fold reduction in viral titers at a concentration of 0.31 mg/mL; no viral titer results are reported for concentrations of 0.62 mg/mL and higher[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
-
Cell Line:mouse neuroblastoma NA cells (ATCC clone C-1300)
-
Concentration:0.155 mg/mL, 0.31 mg/mL, 0.62 mg/mL, 1.25 mg/mL, 2.5 mg/mL, 5.0 mg/mL
-
Incubation Time:24 h
-
Result:Showed no effect on NA cell monolayers at concentrations up to 0.31 mg/mL.
Caused small cytopathic effects affecting 10% of cells at 0.62 mg/mL.
Induced cytopathic effects affecting 25% of cells at 1.25 mg/mL.
Provoked intense morphological alterations affecting more than 50% of cells at 2.5 mg/mL and 5.0 mg/mL.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Swiss mice (male, 2 months old, 18-22 g)[1]
-
Dosage:220 mg/kg; 380 mg/kg; 720 mg/kg; 1000 mg/kg
-
Administration:i.p.; single dose
-
Result:Caused no mortality in mice.
Showed no macroscopic or microscopic alterations (including inflammation, granulomas, or lesions) in liver, kidney, thymus, spleen, or heart tissue relative to vehicle controls.
Chemical Information
-
CAS No. 146-90-7
-
Molecular Weight 286.24
-
Formula C14H10N2O5
-
SMILES
O=C(C(C1=NC2=C(CO)C=CC=C2OC1=C3)=C(N)C3=O)O
-
Structure Classification
-
Initial Source
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
-
Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
-
Gram Staining of Tissue Sections
Gram staining of tissue sections is a histochemical technique used to differentiate Gram-positive and Gram-negative bacteria within histological specimens based on differences in bacterial cell wall structure and dye retention, adapted from classical bacteriological Gram staining into tissue-compatible “histological Gram stain” variants. In tissue applications, modifications of the Brown-Hopps and Brown-Brenn methods are commonly used to improve differentiation of microorganisms embedded within host connective tissue and to reduce overstaining or loss of Gram-negative signal, which are known limitations of earlier approaches. The principle relies on crystal violet-iodine complex retention in Gram-positive organisms and subsequent decolorization and counterstaining steps that allow contrast visualization of Gram-negative organisms against tissue background.
-
Bacterial live/dead nucleic-acid viability staining
The LIVE/DEAD bacterial viability staining method is based on differential permeability of nucleic-acid-binding fluorescent dyes, most commonly SYTO 9 and propidium iodide (PI), which enables discrimination of bacterial populations with intact versus compromised cytoplasmic membranes. SYTO 9 penetrates both intact and damaged bacterial membranes and binds nucleic acids to produce green fluorescence, whereas propidium iodide penetrates only cells with compromised membranes and fluoresces red while also reducing SYTO 9 signal through competitive binding and fluorescence interactions. The resulting fluorescence pattern is interpreted as a proxy for membrane integrity, which is widely used as an indicator of bacterial viability in microscopy, flow cytometry, and spectroscopic platforms. However, mechanistic studies show that SYTO 9 and PI interactions involve displacement and fluorescence resonance energy transfer effects, which can influence signal interpretation depending on dye ratios a
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Cinnabarin Related Classifications
HY-N14107 Related Classifications
Powered by Bioz