Guanylyl Imidodiphosphate trisodium  (Synonyms: Guanosine 5'-[β,γ-imido]triphosphate trisodium)

Guanylyl Imidodiphosphate (Guanosine 5'-[β,γ-imido]triphosphate) trisodium is a non-hydrolysable GTP analogue, an activator of ADP-ribosylation factor (ARF) and a potent stimulator of adenylate cyclase. Imidodiphosphate trisodium can be used in protein synthesis studies^[1][2][3].

Guanylyl Imidodiphosphate trisodium (GppNHp) (100 μM) induces the secretion of dense granules from permeabilized platelets in response to calcium ion stimulation, and GTP-Ral enhances the calcium sensitivity of permeabilized platelet dense granule secretion which is inhibited by Sec5-RBD^[1].
Guanylyl Imidodiphosphate trisodium (GppNHp) (0.017-333 μM) causes a significant reduction in the lag time required to stimulate adenylate cyclase activity^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

589.13

C₁₀H₁₃N₅Na₃O₁₄P₃

148892-91-5

Solid

White to off-white

O=C1C(N=CN2[C@H]3[C@H](O)[C@H](O)[C@@H](COP(OP(OP(O)(O[Na])=O)(O[Na])=O)(O[Na])=O)O3)=C2N=C(N)N1

Shipping with dry ice.

-80°C, stored under nitrogen

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (stored under nitrogen). When stored at -80°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Mitsunori Kawato, et al. Regulation of platelet dense granule secretion by the Ral GTPase-exocyst pathway. J Biol Chem. 2008 Jan 4;283(1):166-174.  [Content Brief]

  [2]. J Goldberg, et al. Structural basis for activation of ARF GTPase: mechanisms of guanine nucleotide exchange and GTP-myristoyl switching. Cell. 1998 Oct 16;95(2):237-48.  [Content Brief]

  [3]. C Gaudin, et al. Overexpression of Gs alpha protein in the hearts of transgenic mice. J Clin Invest. 1995 Apr;95(4):1676-83.  [Content Brief]