SQ22536
Based on 41 publication(s) in Google Scholar
SQ22536 is an effective adenylate cyclase (AC) inhibitor.
For research use only. We do not sell to patients.
- Purity: 99.81%
- CAS No.: 17318-31-9
- Formula: C9H11N5O
- Molecular Weight:205.22
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) SQ22536
More- Mol Plant. 2025 Sep 1;18(9):1549-1566. [Abstract]
- Nat Commun. 2024 Sep 12;15(1):7996. [Abstract]
- J Exp Clin Cancer Res. 2024 Apr 4;43(1):105. [Abstract]
- Cell Death Dis. 2020 May 26;11(5):394. [Abstract]
- Genome Biol. 2025 Sep 4;26(1):267. [Abstract]
- Dev Cell. 2024 Dec 5:S1534-5807(24)00698-1. [Abstract]
- Int J Biol Macromol. 2024 Aug 15:134783. [Abstract]
- Phytomedicine. 2024 Dec:135:156166. [Abstract]
- Phytomedicine. 2023 Oct:119:154982. [Abstract]
- J Agric Food Chem. 2026 Apr 22;74(15):12132-12144. [Abstract]
- J Agric Food Chem. 2023 Nov 22;71(46):17615-17626. [Abstract]
- J Agric Food Chem. 2020 Jun 24;68(25):6864-6872. [Abstract]
- Prog Neurobiol. 2021 Jul:202:102041. [Abstract]
- J Bone Miner Res. 2023 Apr;38(4):597-614. [Abstract]
- Chin Med. 2023 Oct 10;18(1):128. [Abstract]
- Int J Mol Sci. 2023 Jan 16;24(2):1771. [Abstract]
- Int J Mol Sci. 2022 Oct 20;23(20):12595. [Abstract]
- Int J Mol Sci. 2022 Sep 14;23(18):10709. [Abstract]
- Eur J Pharmacol. 2026 Feb 10:1014:178523. [Abstract]
- Invest Ophthalmol Vis Sci. 2025 Dec 1;66(15):16. [Abstract]
- Int Immunopharmacol. 2023 Jan:114:109507. [Abstract]
- iScience. 2021 Jul 1;24(7):102812. [Abstract]
- Antiviral Res. 2023 Jul:215:105635. [Abstract]
- Sci Rep. 2025 Jul 1;15(1):20426. [Abstract]
- Clin Immunol. 2026 Jun:285:110706. [Abstract]
- Exp Cell Res. 2023 Feb 15;423(2):113471. [Abstract]
- Dis Model Mech. 2023 Oct 1;16(10):dmm050206. [Abstract]
- Front Oncol. 2020 Mar 31;10:404. [Abstract]
- Korean J Pain. 2023 Jul 1;36(3):335-346. [Abstract]
- Arch Biochem Biophys. 2025 Mar 27:768:110404. [Abstract]
- Neuroscience. 2024 Mar 26:542:11-20. [Abstract]
- J Cell Biochem. 2019 Jan;120(1):321-331. [Abstract]
- J Exp Biol. 2025 Sep 25:jeb.250571. [Abstract]
- J Nat Med. 2022 Jan;76(1):188-199. [Abstract]
- J Neurophysiol. 2024 Jan 1;131(1):64-74. [Abstract]
- bioRxiv. 2025 Nov 28.
- bioRxiv. 2025 April 26.
- Res Sq. 2025 Apr 08.
- bioRxiv. 2024 Oct 22:2024.10.18.619152. [Abstract]
- Res Sq. 2024 Jun 11.
- J Basic Clin Physiol Pharmacol. 2021 Feb 8;33(3):305-315. [Abstract]
Customer Validation & Images
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Biological Activity
adenylate cyclase (AC)[1]
SQ22536 (SQ22,536) effectively inhibits the effect of forskolin with respective IC50 values of 5 μM.
Preincubation with graded concentrations of SQ22536 reveals that both SQ22536 effectively inhibits PACAP-induced reporter gene activation with approximate IC50 value of 5 μM.
SQ22536 more potently inhibits forskolin-induced Elk activation (IC50=10 μM) than 8-Br-cAMP-induced Elk activation (IC50=170 μM).
Most notably, there are substantial differences in the reported potencies of SQ22536 to inhibit the activities of recombinant AC5 and AC6, with respective IC50 values of 2 μM and 360 μM. At a greater concentration (500 μM), SQ22536 significantly inhibits neurite elongation due to either forskolin or 8-Br-cAMP[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 17318-31-9
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Appearance Solid
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Molecular Weight 205.22
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Formula C9H11N5O
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Color White to off-white
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SMILES
NC1=C2N=CN(C3OCCC3)C2=NC=N1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (41)
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Journal Impact Factor
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Most Recent
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Mol Plant
Arabidopsis HSFA1b functions as a heat sensor inhibiting OST1-mediated stomatal closure through its adenylate-cyclase activity. [Abstract]2025 Sep 1;18(9):1549-1566. PMID: 40745919 -
Nat Commun
Engineered model of heart tissue repair for exploring fibrotic processes and therapeutic interventions. [Abstract]2024 Sep 12;15(1):7996. PMID: 39266508 -
J Exp Clin Cancer Res
GPR65 sensing tumor-derived lactate induces HMGB1 release from TAM via the cAMP/PKA/CREB pathway to promote glioma progression. [Abstract]2024 Apr 4;43(1):105. PMID: 38576043 -
Cell Death Dis
P2Y14 receptor has a critical role in acute gouty arthritis by regulating pyroptosis of macrophages. [Abstract]2020 May 26;11(5):394. PMID: 32457291
SQ22536 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 May 26;11(5):394. [Abstract]
Western blotting shows that the activation of synovial NLRP3 inflammasome signaling is markedly provoked by decreased cAMP in SQ22536-treated P2Y14R-KO rats.
SQ22536 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 May 26;11(5):394. [Abstract]
SQ22536 treatment enhances the colocalization intensity of synovial NLRP3 and ASC in MSU-stimulated P2Y14R-KO rats in immunofluorescence staining.
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Genome Biol
Artemisinin alleviates Parkinson's disease by targeting Adcy5-Gch1 axis to trigger a cascade generation of BH4 and dopamine in rats. [Abstract]2025 Sep 4;26(1):267. PMID: 40908481 -
Dev Cell
Collective oscillatory signaling in Dictyosteliumdiscoideum acts as a developmental timer initiated by weak coupling of a noisy pulsatile signal. [Abstract]2024 Dec 5:S1534-5807(24)00698-1. PMID: 39672161 -
Int J Biol Macromol
An acidic polysaccharide promoting GLP-1 secretion from Dendrobium huoshanense protocorm-like bodies: Structure validation and activity exploration. [Abstract]2024 Aug 15:134783. PMID: 39153673 -
Phytomedicine
Tong-Qiao-Huo-Xue Decoction promotes synaptic remodeling via cAMP/PKA/CREB pathway in vascular dementia rats. [Abstract]2024 Dec:135:156166. PMID: 39461198 -
Phytomedicine
Red ginseng extracts ameliorate high-fat diet-induced obesity and insulin resistance by activating the intestinal TGR5-mediated bile acids signaling pathway. [Abstract]2023 Oct:119:154982. PMID: 37531904 -
J Agric Food Chem
Preventive Effect of Caffeic Acid Phenethyl Ester, an Active Component of Propolis, against TNF-α-Induced Endothelial Dysfunction through the β2-Adrenoceptor-Mediated eNOS/NO Signal Pathway. [Abstract]2026 Apr 22;74(15):12132-12144. PMID: 41957988 -
J Agric Food Chem
Galacto-Oligosaccharides Alleviate LPS-Induced Immune Imbalance in Small Intestine through Regulating Gut Microbe Composition and Bile Acid Pool. [Abstract]2023 Nov 22;71(46):17615-17626. PMID: 37947505 -
J Agric Food Chem
Polygonatum cyrtonema Hua Polysaccharide Promotes GLP-1 Secretion from Enteroendocrine L-Cells through Sweet Taste Receptor-Mediated cAMP Signaling. [Abstract]2020 Jun 24;68(25):6864-6872. PMID: 32456438 -
Prog Neurobiol
2021 Jul:202:102041. PMID: 33766679 -
J Bone Miner Res
Bone deterioration in response to chronic high-altitude hypoxia is attenuated by a pulsed electromagnetic field via the primary cilium/HIF-1α axis. [Abstract]2023 Apr;38(4):597-614. PMID: 36680558 -
Chin Med
Isoforskolin modulates AQP4-SPP1-PIK3C3 related pathway for chronic obstructive pulmonary disease via cAMP signaling. [Abstract]2023 Oct 10;18(1):128. PMID: 37817209 -
Int J Mol Sci
Alkaline Dilution Alters Sperm Motility in Dairy Goat by Affecting sAC/cAMP/PKA Pathway Activity. [Abstract]2023 Jan 16;24(2):1771. PMID: 36675287 -
Int J Mol Sci
Asprosin in the Paraventricular Nucleus Induces Sympathetic Activation and Pressor Responses via cAMP-Dependent ROS Production. [Abstract]2022 Oct 20;23(20):12595. PMID: 36293450 -
Int J Mol Sci
Sinomenine Hydrochloride Promotes TSHR-Dependent Redifferentiation in Papillary Thyroid Cancer. [Abstract]2022 Sep 14;23(18):10709. PMID: 36142613 -
Eur J Pharmacol
Tirzepatide reduces intracellular lipid content by promoting the browning of white fat via the cAMP signaling pathway. [Abstract]2026 Feb 10:1014:178523. PMID: 41506434 -
Invest Ophthalmol Vis Sci
Effect of Dipyridamole on Experimental Autoimmune Uveitis: Reprogrammed Immune Cell Landscape and Reduced Th17 Pathogenicity. [Abstract]2025 Dec 1;66(15):16. PMID: 41334959 -
Int Immunopharmacol
HQL6 serves as a novel P2Y14 receptor antagonist to ameliorate acute gouty arthritis through inhibiting macrophage pyroptosis. [Abstract]2023 Jan:114:109507. PMID: 36462336 -
iScience
Celsr3 is required for Purkinje cell maturation and regulates cerebellar postsynaptic plasticity. [Abstract]2021 Jul 1;24(7):102812. PMID: 34308297 -
Antiviral Res
Artesunate inhibits PDE4 leading to intracellular cAMP accumulation, reduced ERK/MAPK signaling, and blockade of influenza A virus vRNP nuclear export. [Abstract]2023 Jul:215:105635. PMID: 37192683 -
Sci Rep
ADCY4 inhibits cAMP-induced growth of breast cancer by inactivating FAK/AKT and ERK signaling but is frequently silenced by DNA methylation. [Abstract]2025 Jul 1;15(1):20426. PMID: 40594405 -
Clin Immunol
Short-chain fatty acid deficiency drives aberrant B cell differentiation in systemic lupus erythematosus. [Abstract]2026 Jun:285:110706. PMID: 41974394 -
Exp Cell Res
VPAC2 receptor mediates VIP-potentiated insulin secretion via ion channels in rat pancreatic β cells. [Abstract]2023 Feb 15;423(2):113471. PMID: 36642263 -
Dis Model Mech
Glycosphingolipids are linked to elevated neurotransmission and neurodegeneration in a Drosophila model of Niemann Pick type C. [Abstract]2023 Oct 1;16(10):dmm050206. PMID: 37815467 -
Front Oncol
Nevirapine Increases Sodium/Iodide Symporter-Mediated Radioiodide Uptake by Activation of TSHR/cAMP/CREB/PAX8 Signaling Pathway in Dedifferentiated Thyroid Cancer. [Abstract]2020 Mar 31;10:404. PMID: 32300552
SQ22536 purchased from MedChemExpress. Usage Cited in: Front Oncol. 2020 Mar 31;10:404. [Abstract]
The expressions of cAMP, pCREB, PAX8, and NIS in WRO 82-1 and dFTC-133 cells treated with or without 200 μM Nevirapine and SQ22536 (10 μM) for 72 h are determined by western blot and analyzed quantitatively.
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Korean J Pain
Perampanel ameliorates nitroglycerin-induced migraine through inhibition of the cAMP/PKA/CREB signaling pathway in the trigeminal ganglion in rats. [Abstract]2023 Jul 1;36(3):335-346. PMID: 37394274 -
Arch Biochem Biophys
The odorant (R)-(-)-carvone promotes glucose-stimulated insulin secretion via the olfactory receptor Olfr1259 in pancreatic β-TC6 cells. [Abstract]2025 Mar 27:768:110404. PMID: 40157529 -
Neuroscience
Acute Administration of Lactate Exerts Antidepressant-like Effect Through cAMP-dependent Protein Synthesis. [Abstract]2024 Mar 26:542:11-20. PMID: 38336096 -
J Cell Biochem
Fsk and IBMX inhibit proliferation and proapoptotic of glioma stem cells via activation of cAMP signaling pathway. [Abstract]2019 Jan;120(1):321-331. PMID: 30171713
SQ22536 purchased from MedChemExpress. Usage Cited in: J Cell Biochem. 2019 Jan;120(1):321-331. [Abstract]
The combination of Fsk and IBMX (Fsk-IBMX) inhibits the expression of cAMP related protein. The results of Western blot in glioma stem cells (GSCs).
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J Exp Biol
Olfactory detection of ursodeoxycholic acid (UDCA) in large yellow croaker (Larimichthys crocea): responses, pathways and receptor functions. [Abstract]2025 Sep 25:jeb.250571. PMID: 40995806 -
J Nat Med
Magnolol upregulates CHRM1 to attenuate Amyloid-β-triggered neuronal injury through regulating the cAMP/PKA/CREB pathway. [Abstract]2022 Jan;76(1):188-199. PMID: 34705126 -
J Neurophysiol
cAMP-dependent protein kinase signaling is required for ( 2R,6R)-hydroxynorketamine to potentiate hippocampal glutamatergic transmission. [Abstract]2024 Jan 1;131(1):64-74. PMID: 38050689 -
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bioRxiv
Rapid Hippocampal Synaptic Potentiation Induced by Ketamine Metabolite ( 2R , 6R )-Hydroxynorketamine Persistently Primes Synaptic Plasticity. [Abstract]2024 Oct 22:2024.10.18.619152. PMID: 39484512 -
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J Basic Clin Physiol Pharmacol
Intracellular signaling molecules of nerve tissue progenitors as pharmacological targets for treatment of ethanol-induced neurodegeneration. [Abstract]2021 Feb 8;33(3):305-315. PMID: 33559456
Solvent & Solubility
DMSO : 100 mg/mL (487.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 55 mg/mL (268.01 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.18 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.18 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 25 mg/mL (121.82 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Protocol
HEK293 CRE-luc2P GloResponse luciferase reporter cells are transduced with retroviral vectors expressing rat PAC1hop receptors. Individual cell lines are obtained by limiting dilution cloning, and a clonal PAC1-expressing line is propagated and used for CRE luciferase assays. In brief, HEK293 CRE-luc2P cells are plated in 96-well plates (10,000 cells in 80 μL media per well) in assay media (DMEM supplemented with 1% fetal bovine serum). One day after plating, cells are treated with AC inhibitors (10 μL in assay media/well) for 30 minutes, followed by agonists (10 μL in assay media/well), and are incubated for 4 hours. Luciferase activity is determined after the addition of 100 μL/well Bright-Glo Luciferase Assay Reagent. Luminescence (RLU) is measured in a Victor3 microtiter plate reader after 2 minutes of agitation at room temperature. Cyclic AMP is measured in NS-1 cells. In brief, NS-1 cells are seeded and grown overnight in 96-well plates. The next day, cells are pretreated for 20 minutes in media containing the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (0.5 mM) with or without SQ22536. After pretreatment with inhibitors, cells are stimulated with agonists, added as 10× solutions, for an additional 20 minutes. Intracellular cAMP is then assayed using the cAMP Biotrak enzyme immunoassay kit for measurement of nonacetylated cAMP[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 4.8728 mL | 24.3641 mL | 48.7282 mL | 121.8205 mL |
| 5 mM | 0.9746 mL | 4.8728 mL | 9.7456 mL | 24.3641 mL | |
| 10 mM | 0.4873 mL | 2.4364 mL | 4.8728 mL | 12.1820 mL | |
| 15 mM | 0.3249 mL | 1.6243 mL | 3.2485 mL | 8.1214 mL | |
| 20 mM | 0.2436 mL | 1.2182 mL | 2.4364 mL | 6.0910 mL | |
| 25 mM | 0.1949 mL | 0.9746 mL | 1.9491 mL | 4.8728 mL | |
| 30 mM | 0.1624 mL | 0.8121 mL | 1.6243 mL | 4.0607 mL | |
| 40 mM | 0.1218 mL | 0.6091 mL | 1.2182 mL | 3.0455 mL | |
| 50 mM | 0.0975 mL | 0.4873 mL | 0.9746 mL | 2.4364 mL | |
| 60 mM | 0.0812 mL | 0.4061 mL | 0.8121 mL | 2.0303 mL | |
| 80 mM | 0.0609 mL | 0.3046 mL | 0.6091 mL | 1.5228 mL | |
| 100 mM | 0.0487 mL | 0.2436 mL | 0.4873 mL | 1.2182 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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