Corisin
Based on 1 Customer Validation
Corisin is a pro-apoptotic small peptide produced by Staphylococcus species. Corisin binds to serum albumin to target organs such as the lungs and kidneys, induces cellular senescence, apoptosis and epithelial-mesenchymal transition, and accelerates the progression of organ fibrosis including pulmonary fibrosis and diabetic renal fibrosis. Corisin levels are closely associated with coronavirus disease 2019 (COVID-19), diabetic chronic kidney disease (CKD), non-diabetic CKD, and idiopathic pulmonary fibrosis (IPF).
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- 純度 : 99.45%
- CAS 番号: 2651277-02-8
- 分子式: C84H133N25O28S
- 分子量:1973.17
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保管条件:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
体外実験
Corisin (48 h) significantly increases phosphatidylserine externalization in human umbilical vein endothelial cells, and this effect is blocked by anti-Corisin monoclonal antibodies[1].
Corisin (5 μM; 24 h) significantly enhances tissue factor activity in A549 alveolar epithelial cells, THP-1 monocytes and human umbilical vein endothelial cells, and this effect is reversed by anti-Corisin monoclonal antibody or anti-TF antibody[1].
Corisin (5 μM; 24 h) significantly reduces thrombomodulin activity in A549 alveolar epithelial cells, THP-1 monocytes and human umbilical vein endothelial cells, and this effect is reversed by anti-Corisin monoclonal antibodies[1].
Corisin (20-40 μg/mL; 24-48 h) promotes apoptosis, disrupts cell cycle progression and reduces proliferative activity in normal human renal proximal tubular epithelial cells, human Caki-2 renal tubular epithelial cells and normal human podocytes, while anti-Corisin monoclonal antibodies inhibit these effects[2].
Corisin (20-40 μg/mL; 48 h) induces cellular senescence in human Caki-2 renal tubular epithelial cells, normal human primary renal tubular epithelial cells, and normal human primary podocytes, which is characterized by increased senescence-associated β-galactosidase activity, altered expression of senescence marker genes, and abnormal nuclear morphology. This effect is inhibited by anti-Corisin monoclonal antibodies[2].
Corisin (20-40 μg/mL; 48 h) induces epithelial-mesenchymal transition in human normal primary podocytes and human normal primary renal proximal tubular epithelial cells, which is characterized by changes in cell morphology and upregulated expression of mesenchymal marker genes, while anti-corisin monoclonal antibodies inhibit these effects[2].
Stimulation of primary human renal proximal tubular epithelial cells with Corisin (20 μg/mL; 24 h) activates pathways associated with DNA damage response, cellular senescence, senescence-associated secretory phenotype, and myofibroblast-like differentiation[2].
Corisin (0.5-50 μM; 16-48 h) induces dose-dependent apoptosis in A549 alveolar epithelial cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:A549 alveolar epithelial cells
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Concentration:0.5 μM, 5 μM, 50 μM (48 h); 5 μM (16 h)
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Incubation Time:48 h (0.5 μM, 5 μM, 50 μM); 16 h (5 μM)
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Result:Induced apoptosis in a dose-dependent manner.
Increased Annexin V-positive cells to 13.7% at 0.5 μM, 21.1% at 5 μM, and 22.7% at 50 μM, all significantly higher than the control 6.2%.
Confirmed apoptotic morphology via transmission electron microscopy at 5 μM.
体内実験
Corisin (administered intratracheally; once daily for 2 consecutive days) induces acute exacerbation of pulmonary fibrosis in TGFβ1 TG mice, which is characterized by significant increases in pulmonary inflammation, collagen deposition, levels of profibrotic mediators, and epithelial cell apoptosis[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:TGFβ1 transgenic (female, 9 weeks old)[2]
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Dosage:5 mg/kg
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Administration:i.p.; thrice weekly; 2 weeks
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Result:Elevated urinary levels of corisin, albumin, and albumin-to-creatinine ratio significantly compared to scrambled peptide-treated mice.
Elevated plasma blood urea nitrogen (BUN) and urinary liver-type fatty acid-binding protein (L-FABP) levels significantly compared to scrambled peptide-treated mice.
Elevated plasma levels of lipopolysaccharide-induced CXC chemokine (LIX/CXCL5), macrophage inflammatory protein-2 (MIP-2), interleukin-1β (IL-1β), platelet-derived growth factor (PDGF), tissue factor (TF), and plasminogen activator inhibitor-1 (PAI-1) significantly compared to scrambled peptide-treated mice.
Increased mean circulating corisin levels to 2.2-fold higher than those in diabetic CKD patients and 1.6-fold higher than those in diabetic TGFβ1 transgenic mice.
Increased renal fibrotic changes significantly compared to scrambled peptide-treated mice.
化学情報
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CAS 番号 2651277-02-8
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性状 Solid
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分子量 1973.17
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分子式 C84H133N25O28S
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Color White to off-white
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配列
Ile-Val-Met-Pro-Glu-Ser-Ser-Gly-Asn-Pro-Asn-Ala-Val-Asn-Pro-Ala-Gly-Tyr-Arg
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シーケンスの短縮
IVMPESSGNPNAVNPAGYR
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
体外:
DMSO : 25 mg/mL (12.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (1.27 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
プロトコル
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Fibrosis/Collagen Morphometry
Fibrosis and collagen morphometry is based on the quantitative visualization of fibrillar collagen deposition in tissue sections using histochemical stains such as Sirius Red (Picrosirius Red) or Masson's trichrome, followed by image-based or polarization-enhanced analysis to estimate collagen proportional area as a surrogate of extracellular matrix accumulation during fibrotic remodeling. Sirius Red combined with polarized light microscopy enhances detection of collagen fibers due to birefringence properties, enabling more specific visualization of collagen type I and III fibrils compared to conventional bright-field histology, while whole-section or region-restricted digital morphometry reduces field-selection bias in fibrosis assessment. Alternative quantitative approaches include second harmonic generation (SHG) and two-photon excited fluorescence microscopy, which enable label-free detection of fibrillar collagen and have been validated against histological staining and biochemica
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Collagen: Sirius Red Staining
Sirius Red or picrosirius red staining is a histochemical method for visualizing collagen-rich extracellular matrix in tissue sections, and collagen fibers are detected as red-stained structures under bright-field microscopy with enhanced birefringence under polarized light. Picrosirius red is useful for assessing total collagen organization, distribution, and fibrosis burden, but polarized color should not be interpreted as a definitive collagen type I versus type III readout because color is affected by fiber orientation, thickness, and packing.
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Apoptosis
Apoptosis, also called programmed cell death, is generally characterized by distinct morphological characteristics.
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TUNEL staining for apoptotic DNA fragmentation
TUNEL staining detects DNA strand breaks by using terminal deoxynucleotidyl transferase to add labeled nucleotides to exposed 3′-OH DNA termini, generating either microscopic staining in fixed cells or tissue sections, or fluorescence/cytometric signal in cell suspensions. TUNEL positivity reflects DNA fragmentation but should not be interpreted alone as definitive apoptosis, because TUNEL can also label necrotic, autolytic, mechanically damaged, or DNA-repair-associated DNA breaks.
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Annexin V plus membrane-impermeant dye apoptosis staining
Annexin V-based apoptosis assays rely on the detection of phosphatidylserine (PS) externalization from the inner leaflet of the plasma membrane to the outer leaflet, an early biochemical hallmark of apoptosis. Fluorescently labeled Annexin V binds PS in a calcium-dependent manner, enabling identification of early apoptotic cells by flow cytometry or fluorescence microscopy. When combined with a membrane-impermeant DNA-binding dye (e. g. , propidium iodide), this approach allows discrimination between viable (Annexin V−/dye−), early apoptotic (Annexin V+/dye−), and late apoptotic or necrotic (Annexin V+/dye+) cell populations by assessing membrane integrity and PS exposure.
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Connective Tissue: Masson's Trichrome/Collagen Trichrome Staining
Masson’s Trichrome (collagen/trichrome staining) is a histological technique that differentially stains tissue compartments using sequential acidic dyes to distinguish collagen from muscle and cytoplasmic components based on dye affinity and tissue permeability differences, enabling visualization of fibrosis and connective tissue architecture in histological sections. The classical formulation typically uses Weigert's iron hematoxylin for nuclear staining, Biebrich scarlet-acid fuchsin for cytoplasm and muscle, and aniline blue (or light green variants) for collagen, producing a characteristic blue/green collagen signal contrasted against red cytoplasm and dark nuclei. The staining principle relies on selective displacement of smaller dye molecules by larger anionic dyes in collagen-rich regions under controlled acidified conditions, which enhances collagen-specific dye retention. This property makes the method widely used for fibrosis assessment in organs such as heart, liver, lung, a
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Apoptosis Solutions
Apoptosis is a regulated, generally non-lytic cell-death pathway that removes unwanted, damaged, infected, or abnormal cells through coordinated morphological changes, caspase activation, DNA fragmentation, and membrane remodeling. The intrinsic apoptosis pathway is controlled mainly by mitochondrial outer membrane permeabilization, BCL-2 family proteins, cytochrome c release, apoptosome formation, caspase-9 activation, and downstream executioner caspase-3/7 activation. The extrinsic apoptosis pathway is initiated by death receptors such as Fas, TNFR, and TRAIL receptors, which recruit adaptor proteins and activate caspase-8 before engaging executioner caspases or mitochondrial amplification through BID cleavage. Apoptosis is linked to many phenotypes, including cancer cell killing, tissue homeostasis, immune regulation, neurodegeneration, infection response, and treatment-induced cytotoxicity; unresolved questions include how apoptosis interacts with necroptosis, pyroptosis, ferroptos
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Senescence-associated β-galactosidase staining
Senescence-associated β-galactosidase staining detects β-galactosidase activity that is histochemically visible at pH 6. 0 in senescent cells, where X-gal cleavage produces an insoluble blue precipitate observable by bright-field microscopy. This activity reflects increased lysosomal β-galactosidase/lysosomal mass rather than a senescence-essential enzyme, because GLB1 depletion or genetic lysosomal β-galactosidase deficiency can abolish SA-β-gal staining while cells still undergo senescence. SA-β-gal was originally reported in senescent but not presenescent fibroblasts and keratinocytes, absent from quiescent fibroblasts and terminally differentiated keratinocytes, and increased with donor age in human skin samples. Because SA-β-gal can also appear in some non-senescent or tissue-specific contexts, interpretation should be paired with experimental controls and, when possible, independent senescence markers.
純度とドキュメンテーション
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データシート (281 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Tsuruga T, et al. Role of microbiota-derived corisin in coagulation activation during SARS-CoV-2 infection. J Thromb Haemost. 2024;22(7):1919-1935. [Content Brief]
[2]. Yasuma T, et al. Microbiota-derived corisin accelerates kidney fibrosis by promoting cellular aging. Nat Commun. 2025;16(1):7591. Published 2025 Aug 25. [Content Brief]
[3]. D'Alessandro-Gabazza CN, et al. A Staphylococcus pro-apoptotic peptide induces acute exacerbation of pulmonary fibrosis. Nat Commun. 2020;11(1):1539. Published 2020 Mar 24. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.5068 mL | 2.5340 mL | 5.0680 mL | 12.6700 mL |
| 5 mM | 0.1014 mL | 0.5068 mL | 1.0136 mL | 2.5340 mL | |
| 10 mM | 0.0507 mL | 0.2534 mL | 0.5068 mL | 1.2670 mL |
Corisin 関連分類
HY-P11178 関連分類
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