Vacquinol-1
Based on 1 Customer Validation
Vacquinol-1 (NSC13316) is a MKK4-specific activator that activates the MAPK pathway. Vacquinol-1 inhibits the growth, migration and colony formation, and induces apoptosis of cancer cells. Vacquinol-1 is applicable to research related to cancers such as hepatocellular carcinoma.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.33%
- CAS 番号: 5428-80-8
- 分子式: C21H21ClN2O
- 分子量:352.86
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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生物活性
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| U3013MG | IC50 |
3.14 μM
Compound: 1
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Cytotoxicity against patient-derived U3013MG cells assessed as reduction in cell viability by Celltiter-Glo reagent based assay
Cytotoxicity against patient-derived U3013MG cells assessed as reduction in cell viability by Celltiter-Glo reagent based assay
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[PMID: 27607569] |
Vacquinol-1 (1.56-25 μM; 24-72 h) inhibits the viability of BEL7402/Huh7 hepatocellular carcinoma cells in vitro[1].
Vacquinol-1 (0-24 μM; 24 h) inhibits the colony-forming ability of BEL7402/Huh7 hepatocellular carcinoma cells and induces cell apoptosis[1].
Vacquinol-1 (10 μM; 24 h) activates the endogenous apoptotic pathway in BEL7402 hepatocellular carcinoma cells, induces the cleavage of caspase-3, caspase-9 and PARP-1, upregulates the expression of Bax and Bim, and downregulates the expression of Bcl-2[1].
Vacquinol-1 (10 μM; 1-12 h) induces the translocation of the pro-apoptotic protein Bax from the cytoplasm to mitochondria in BEL7402 hepatocellular carcinoma cells[1].
Vacquinol-1 (15 μM; 5 h) activates the JNK and p38 MAPK pathways, upregulates α-SMA, an activation marker of the hepatic stellate cell line HSC-T6, promotes collagen production, and induces phosphorylation of the linker region of Smad2/3[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:BEL7402 (hepatocellular carcinoma, HCC)
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Concentration:1.56 μM, 3.13 μM, 6.26 μM, 12.5 μM, 25 μM
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Incubation Time:24, 48, 72 h
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Result:Inhibited BEL7402 cell proliferation in a dose-dependent manner.
Reached half maximal inhibitory concentration (IC50) values of 12.35 μM (24 h), 11.48 μM (48 h), and 10.21 μM (72 h).
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Cell Line:Huh7 (hepatocellular carcinoma, HCC)
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Concentration:1.56 μM, 3.13 μM, 6.26 μM, 12.5 μM, 25 μM
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Incubation Time:24, 48, 72 h
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Result:Inhibited Huh7 cell proliferation in a dose-dependent manner.
Reached half maximal inhibitory concentration (IC50) values of 6.21 μM (24 h), 5.58 μM (48 h), and 4.89 μM (72 h).
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Cell Line:BEL7402 (hepatocellular carcinoma, HCC)
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Concentration:10 μM
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Incubation Time:24 h
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Result:Increased the percentage of apoptotic cells.
Resulted in nuclear fragmentation and apoptotic body formation in BEL7402 cells, whereas control cells displayed round, normal nuclei.
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Cell Line:rat hepatic stellate HSC-T6 cells
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Concentration:15 μM
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Incubation Time:5 h
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Result:Upregulated the protein levels of phosphorylated (P)-JNK1/2 and P-p38 (but not P-ERK1/2) in HSC-T6 cells.
Increased the protein levels of α-SMA, Collagen I, P-Smad2L, and P-Smad3L in HSC-T6 cells.
化学情報
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CAS 番号 5428-80-8
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性状 Solid
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分子量 352.86
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分子式 C21H21ClN2O
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Color White to off-white
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SMILES
OC(C1NCCCC1)C2=CC(C3=CC=C(Cl)C=C3)=NC4=CC=CC=C24
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別名
NSC13316
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
DMSO : 12.5 mg/mL (35.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (280 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Sun SL, et al. Vacquinol‑1 induces apoptosis in hepatocellular carcinoma cell. Mol Med Rep. 2018;18(1):557-563. [Content Brief]
[2]. Wu C, et al. Salvianolic acid B exerts anti-liver fibrosis effects via inhibition of MAPK-mediated phospho-Smad2/3 at linker regions in vivo and in vitro. Life Sci. 2019;239:116881. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8340 mL | 14.1699 mL | 28.3399 mL | 70.8496 mL |
| 5 mM | 0.5668 mL | 2.8340 mL | 5.6680 mL | 14.1699 mL | |
| 10 mM | 0.2834 mL | 1.4170 mL | 2.8340 mL | 7.0850 mL | |
| 15 mM | 0.1889 mL | 0.9447 mL | 1.8893 mL | 4.7233 mL | |
| 20 mM | 0.1417 mL | 0.7085 mL | 1.4170 mL | 3.5425 mL | |
| 25 mM | 0.1134 mL | 0.5668 mL | 1.1336 mL | 2.8340 mL | |
| 30 mM | 0.0945 mL | 0.4723 mL | 0.9447 mL | 2.3617 mL |
Vacquinol-1 関連分類
HY-19345 関連分類
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