MDL 72527 dihydrochloride

Name: MDL 72527 dihydrochloride
Brand: MedChemExpress
SKU: HY-100621
Rating: 4.5 (1 reviews)

Cat. No.: HY-100621 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

MDL 72527 dihydrochloride is a potent polyamine oxidase (PAO) inhibitor. MDL 72527 dihydrochloride shows a lysosomotropic effect. MDL 72527 dihydrochloride shows neuroprotective effects.

For research use only. We do not sell to patients.

MDL 72527 dihydrochloride Chemical Structure

CAS No. : 93565-01-6

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5 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 320	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 520	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 830	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE MDL 72527 dihydrochloride

•Proc Natl Acad Sci U S A. 2024 Mar 26;121(13):e2319429121. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

MDL 72527 dihydrochloride is a potent polyamine oxidase (PAO) inhibitor. MDL 72527 dihydrochloride shows a lysosomotropic effect. MDL 72527 dihydrochloride shows neuroprotective effects^[1]^[2].

IC₅₀ & Target

polyamine oxidase (PAO)^[1]

In Vitro

MDL 72527 dihydrochloride (300 µM; 24 h) shows evident morphological changes with the appearance of a large number of lysosomes and numerous cytoplasmic vacuoles in M14 WT cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[2]

Cell Line:	M14 cells
Concentration:	300 µM
Incubation Time:	24 h
Result:	Showed no significant effect on cell survival of melanoma cells.

In Vivo

MDL 72527 dihydrochloride (100 mg/kg; i.p.; once) shows neuroprotective against neuronal cell damage after temporary focal cerebral ischemia^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	250-300 g, Male spontaneously hypertensive rats (SHRs)^[1]
Dosage:	100 mg/kg
Administration:	I.p.; after the induction of ischemia
Result:	Significantly reduced meanarterial blood pressure, changed the brain edema formation from 85.7 ± 0.3 to 84.5 ± 0.9% in cortex and from 79.9 ± 1.7 to 78.4 ± 2.0% in subcortex (difference not significant), reduced ischemic injury volume by 22% in the cortex and 17% in the subcortex.

Molecular Weight

265.22

Formula

C₁₂H₂₂Cl₂N₂

CAS No.

93565-01-6

Appearance

Solid

Color

Light brown to brown

SMILES

C=C=CCNCCCCNCC=C=C.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (377.05 mM; Need ultrasonic)

DMSO : 12.5 mg/mL (47.13 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7705 mL	18.8523 mL	37.7045 mL
5 mM	0.7541 mL	3.7705 mL	7.5409 mL
10 mM	0.3770 mL	1.8852 mL	3.7705 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (4.71 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (4.71 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: Saline
Solubility: 50 mg/mL (188.52 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (270 KB) SDS (393 KB)

COA (242 KB) HNMR (121 KB) MS (80 KB)

Handling Instructions (2659 KB)

References

[1]. Doğan A, et al. Effects of MDL 72527, a specific inhibitor of polyamine oxidase, on brain edema, ischemic injury volume, and tissue polyamine levels in rats after temporary middle cerebral artery occlusion. J Neurochem. 1999 Feb;72(2):765-70. [Content Brief]

[2]. Agostinelli E, et al. Toxicity of enzymatic oxidation products of spermine to human melanoma cells (M14): sensitization by heat and MDL 72527. Biochim Biophys Acta. 2006 Oct;1763(10):1040-50. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	3.7705 mL	18.8523 mL	37.7045 mL	94.2614 mL
	5 mM	0.7541 mL	3.7705 mL	7.5409 mL	18.8523 mL
	10 mM	0.3770 mL	1.8852 mL	3.7705 mL	9.4261 mL
	15 mM	0.2514 mL	1.2568 mL	2.5136 mL	6.2841 mL
	20 mM	0.1885 mL	0.9426 mL	1.8852 mL	4.7131 mL
	25 mM	0.1508 mL	0.7541 mL	1.5082 mL	3.7705 mL
	30 mM	0.1257 mL	0.6284 mL	1.2568 mL	3.1420 mL
	40 mM	0.0943 mL	0.4713 mL	0.9426 mL	2.3565 mL
H₂O	50 mM	0.0754 mL	0.3770 mL	0.7541 mL	1.8852 mL
	60 mM	0.0628 mL	0.3142 mL	0.6284 mL	1.5710 mL
	80 mM	0.0471 mL	0.2357 mL	0.4713 mL	1.1783 mL
	100 mM	0.0377 mL	0.1885 mL	0.3770 mL	0.9426 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.