N-Acetyl-DL-methionine
Based on 1 Customer Validation
N-Acetyl-DL-methionine is an orally active methionine analog and a glutathione (GSH) precursor that can be metabolized into cysteine in vivo. N-Acetyl-DL-methionine increases plasma methionine concentration and the molar percentage of plasma lysine in castrated sheep, and can partially meet the methionine requirement of growing rats. N-Acetyl-DL-methionine is absorbable from the lower digestive tract of sheep and undergoes ruminal microbial degradation in vivo. N-Acetyl-DL-methionine alleviates Acetaminophen (HY-66005)-induced hepatic GSH depletion in mice, maintains hepatocyte integrity, and promotes de novo synthesis of hepatic GSH. N-Acetyl-DL-methionine can be used in studies related to acetaminophen-induced liver injury.
For research use only. We do not sell to patients.
- Purity: 98.40%
- CAS No.: 1115-47-5
- Formula: C7H13NO3S
- Molecular Weight:191.25
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
N-Acetyl-DL-methionine (NAM) is resistant to degradation by rumen microorganisms in vitro[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
N-Acetyl-DL-methionine delivered via single oral administration at 6.44 g shows a 15.7% recovery rate in the abomasal digesta of healthy castrated sheep. The recovery rate of dietary methionine is significantly lower than that of the control group, whereas no significant difference in total abomasal methionine equivalents is detected between the control group and the DL-homocysteine thiolactone-treated group[1].
N-Acetyl-DL-methionine combined orally with 400 mg/kg acetaminophen (HY-66005) in one single dose can remarkably relieve hepatic GSH depletion and maintain hepatocyte integrity induced by acetaminophen[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Bom:NMRI (male, >20 days, 35-50 g, paracetamol-induced hepatotoxicity)[2]
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Dosage:Equimolar to 400 mg/kg paracetamol
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Administration:p.o.; single co-administered dose with paracetamol
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Result:Maintained hepatic GSH content at 42% of pre-dose level at 1 hour, 78% at 4 hours, 66% at 8 hours, and significantly above 16-hour control levels at 16 hours post-dose.
Showed plasma ALAT time-average values not significantly different from control levels, with no statistically significant transitory elevation observed.
Achieved survival rate of 5/5 at 0, 1, 4, 8, and 12 hours, and 4/5 at 16 hours (one spontaneous death unrelated to treatment).
Chemical Information
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CAS No. 1115-47-5
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Appearance Solid
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Molecular Weight 191.25
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Formula C7H13NO3S
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Color White to off-white
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SMILES
OC(C(CCSC)NC(C)=O)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
H2O : 125 mg/mL (653.59 mM; Need ultrasonic)
DMSO : 100 mg/mL (522.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.07 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.07 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO / H2O | 1 mM | 5.2288 mL | 26.1438 mL | 52.2876 mL | 130.7190 mL |
| 5 mM | 1.0458 mL | 5.2288 mL | 10.4575 mL | 26.1438 mL | |
| 10 mM | 0.5229 mL | 2.6144 mL | 5.2288 mL | 13.0719 mL | |
| 15 mM | 0.3486 mL | 1.7429 mL | 3.4858 mL | 8.7146 mL | |
| 20 mM | 0.2614 mL | 1.3072 mL | 2.6144 mL | 6.5359 mL | |
| 25 mM | 0.2092 mL | 1.0458 mL | 2.0915 mL | 5.2288 mL | |
| 30 mM | 0.1743 mL | 0.8715 mL | 1.7429 mL | 4.3573 mL | |
| 40 mM | 0.1307 mL | 0.6536 mL | 1.3072 mL | 3.2680 mL | |
| 50 mM | 0.1046 mL | 0.5229 mL | 1.0458 mL | 2.6144 mL | |
| 60 mM | 0.0871 mL | 0.4357 mL | 0.8715 mL | 2.1786 mL | |
| 80 mM | 0.0654 mL | 0.3268 mL | 0.6536 mL | 1.6340 mL | |
| 100 mM | 0.0523 mL | 0.2614 mL | 0.5229 mL | 1.3072 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
N-Acetyl-DL-methionine Related Classifications
HY-W019704 Related Classifications
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