N-Acetyl-L-methionine
Based on 2 publication(s) in Google Scholar
N-Acetyl-L-methionine is a dietary supplementation. N-Acetyl-L-methionine increases caspase-3 activity and inhibits mitochondrial activity. N-Acetyl-L-methionine promotes lactation and improves meat quality.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 65-82-7
- Formula: C7H13NO3S
- Molecular Weight:191.25
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) N-Acetyl-L-methionine
MoreAll Caspase Isoforms
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Biological Activity
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Caspase-3 |
N-Acetyl-L-methionine (75 μM) reduces the number of chemical modifications and structural changes of recombinant human serum albumin (rHSA) more effectively than N-Acetyl-L-tryptophan when rHSA is oxidized by chloramine-T[2].
N-Acetyl-L-methionine (0-500 µg/mL; 24-72 h) inhibits the growth and mitochondrial activity of Jurkat cells and MTC-SK cells in a concentration- and time- dependent manner[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Jurkat, MTC-SK cells
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Concentration:0, 125, 200, 250, 500 µg/mL
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Incubation Time:24 h, 48 h, 72 h
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Result:Significantly reduced cell growth after 72 h at 125 µg/mL (Jurkat cells).
Reduced cell growth after 24 h at 500 µg/mL.
Achieved the IC50% reduction in cell growth after 72 h at 125 µg/mL.
N-Acetyl-L-methionine (30 g/cow per d; p.o., diet; once daily; 21-d treatment periods with 4 periods in total) increases milk fat concentration and yield as well as 3.5% fat-corrected milk yield in lactating dairy cows[4].
N-Acetyl-L-methionine (0.125%-0.50% of dry matter basis; added to the diet; once daily) improves meat quality by enhancing the antioxidant effect of lipids and proteins, with the optimal dose being 0.25% of dry matter basis in finishing Angus heifers[5].
N-Acetyl-L-methionine (equimolar levels to L-methionine ranging from 0 to 5.0% in the diet; p.o.; ad libitum; 14 days) causes progressive decreases in weight gain of growing rats at levels higher than the beneficial level (0.3% supplemental L-methionine equivalent)[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 65-82-7
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Appearance Solid
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Molecular Weight 191.25
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Formula C7H13NO3S
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Color White to off-white
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SMILES
CSCC[C@H](NC(C)=O)C(O)=O
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Synonyms
N-Acetylmethionine
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (2)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
DMSO : 100 mg/mL (522.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.07 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.07 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Ball RO, et al. The in vivo sparing of methionine by cysteine in sulfur amino acid requirements in animal models and adult humans. J Nutr. 2006 Jun;136(6 Suppl):1682S-1693S. [Content Brief]
[2]. Kouno Y, et al. N-acetyl-L-methionine is a superior protectant of human serum albumin against post-translational oxidation as compared to N-acetyl-L-tryptophan. Biochem Biophys Rep. 2016 Apr 26;6:266-274. [Content Brief]
[3]. Greilberger J, et al. The Influence of N-Acetyl-selenomethionine on Two RONS-Generating Cancer Cell Lines Compared to N-Acetyl-methionine. Cells. 2024 May 29;13(11):937. [Content Brief]
[4]. Fagundes MA, et al. Influence of supplementing a methionine derivative, N-acetyl-l-methionine, in dairy diets on production and ruminal fermentation by lactating cows during early to mid lactation. J Dairy Sci. 2018 Aug;101(8):7082-7094. [Content Brief]
[5]. Liu Y, et al. N-acetyl-l-methionine dietary supplementation improves meat quality by oxidative stability of finishing Angus heifers. Meat Sci. 2024 Aug;214:109499. [Content Brief]
[6]. Rotruck JT, et al. Effects of excess dietary l-methionine and N-acetyl-l-methionine on growing rats. J Nutr. 1977 Mar;107(3):357-62. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.2288 mL | 26.1438 mL | 52.2876 mL | 130.7190 mL |
| 5 mM | 1.0458 mL | 5.2288 mL | 10.4575 mL | 26.1438 mL | |
| 10 mM | 0.5229 mL | 2.6144 mL | 5.2288 mL | 13.0719 mL | |
| 15 mM | 0.3486 mL | 1.7429 mL | 3.4858 mL | 8.7146 mL | |
| 20 mM | 0.2614 mL | 1.3072 mL | 2.6144 mL | 6.5359 mL | |
| 25 mM | 0.2092 mL | 1.0458 mL | 2.0915 mL | 5.2288 mL | |
| 30 mM | 0.1743 mL | 0.8715 mL | 1.7429 mL | 4.3573 mL | |
| 40 mM | 0.1307 mL | 0.6536 mL | 1.3072 mL | 3.2680 mL | |
| 50 mM | 0.1046 mL | 0.5229 mL | 1.0458 mL | 2.6144 mL | |
| 60 mM | 0.0871 mL | 0.4357 mL | 0.8715 mL | 2.1786 mL | |
| 80 mM | 0.0654 mL | 0.3268 mL | 0.6536 mL | 1.6340 mL | |
| 100 mM | 0.0523 mL | 0.2614 mL | 0.5229 mL | 1.3072 mL |
N-Acetyl-L-methionine Related Classifications
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