NRAS-G4-ligand-1
Based on 1 Customer Validation
NRAS-G4-ligand-1 is a NRAS-G4 binder with Kd values of 0.25 μM (AlexaFluor 647-labeled) and 0.932 μM (biotinylated), respectively. NRAS-G4-ligand-1 acts as a translation inhibitor, reduces cancer cell viability, and downregulates NRAS protein levels. NRAS-G4-ligand-1 can be used in the research of melanoma and breast cancer.
For research use only. We do not sell to patients.
- CAS No.: 1185061-66-8
- Formula: C25H22N6O3S
- Molecular Weight:486.55
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
NRAS-G4-ligand-1 (Compound 18) (0-25 μM; 15 min) binds to purified AlexaFluor 647-labeled NRAS-G4 RNA, with a KD of 0.25 μM[1].
NRAS-G4-ligand-1 (0-12 μM; 120 sec association, 200 sec dissociation) binds to immobilized biotinylated NRAS-G4 RNA with a KD of 0.93 μM[1].
NRAS-G4-ligand-1 (0-20 μM; 15 min before translation, 90 min in vitro translation) dose-dependently inhibits the translation of the G4-structured NRAS-G4-FL luciferase reporter transcript in rabbit reticulocyte lysate, while exerting no effect on the G4-free NRAS-G4-Del-FL transcript[1].
NRAS-G4-ligand-1 (0-20 μM; 48 h) induces a moderate (approximately 20%-30%), dose-dependent reduction in NRAS protein levels in MCF-7 and SK-MEL-2 cells at concentrations up to 20 μM[1].
NRAS-G4-ligand-1 (0-2.5 μM; 48 h) reduces the viability of MCF-7 and SK-MEL-2 cancer cells, with IC50 values of 1.1 μM and 0.9 μM, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF-7 (breast adenocarcinoma), SK-MEL-2 (malignant melanoma)
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Concentration:0, 0.5, 1, 1.5, 2, 2.5 μM
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Incubation Time:48 h
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Result:Decreased cell viability in both cell lines, with an IC50 of 1.1 μM for MCF-7 cells and 0.9 μM for SK-MEL-2 cells.
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Cell Line:MCF-7, SK-MEL-2
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Concentration:0, 0.1, 1, 10, 20 μM
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Incubation Time:48 h
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Result:Caused a modest, dose-dependent decrease in NRAS protein levels, with a ~20-30% reduction observed at 20 μM in both cell lines.
Chemical Information
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CAS No. 1185061-66-8
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Appearance Solid
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Molecular Weight 486.55
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Formula C25H22N6O3S
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SMILES
O=S(C1=CC=C(CCNC2=C3C(C=CC=C3)=NC(C4=NN=C(O4)C5=CC=C(C=C5)C)=N2)C=C1)(N)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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