CDC37 Protein, Human (sf9, GST)

The CDC37 protein is a co-chaperone that binds multiple kinases and promotes their interaction with the Hsp90 complex, resulting in stability and enhanced activity. CDC37 Protein, Human (sf9, GST) is the recombinant human-derived CDC37 protein, expressed by Sf9 insect cells , with C-GST labeled tag.

The CDC37 protein serves as a co-chaperone with a pivotal role in facilitating the interaction between numerous kinases and the Hsp90 complex, leading to the stabilization and enhanced activity of these kinases. It functions by inhibiting HSP90AA1 ATPase activity. CDC37 is likely a part of multiple protein complexes, including those containing chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, PPP5C, PTGES3/p23, and client proteins like TSC2, CDK4, AKT, RAF1, and NR3C1. Another complex involving CDC37 comprises chaperones HSP90 and HSP70, co-chaperones PPP5C and TSC1, and client proteins TSC2, CDK4, AKT, RAF1, and NR3C1, but notably lacking STIP1/HOP and PTGES3/p23. CDC37 also forms a distinct complex with Hsp90/HSP90AB1 and CDK6. Its interactions extend to various proteins, including HSP90AA1, AR, CDK4, CDK6, EIF2AK1, RB1, KSR1, and FLCN, FNIP1, and FNIP2. This diverse network of interactions underscores the versatility of CDC37 in regulating the stability and activity of a wide array of kinases and client proteins.

Human

Sf9 insect cells

C-GST

Q16543 (M1-V378)

11140  [NCBI]

Full Length

MVDYSVWDHIEVSDDEDETHPNIDTASLFRWRHQARVERMEQFQKEKEELDRGCRECKRKVAECQRKLKELEVAEGGKAELERLQAEAQQLRKEERSWEQKLEEMRKKEKSMPWNVDTLSKDGFSKSMVNTKPEKTEEDSEEVREQKHKTFVEKYEKQIKHFGMLRRWDDSQKYLSDNVHLVCEETANYLVIWCIDLEVEEKCALMEQVAHQTIVMQFILELAKSLKVDPRACFRQFFTKIKTADRQYMEGFNDELEAFKERVRGRAKLRIEKAMKEYEEEERKKRLGPGGLDPVEVYESLPEELQKCFDVKDVQMLQDAISKMDPTDAKYHMQRCIDSGLWVPNSKASEAKEGEEAGPGDPLLEAVPKTGDEKDVSV

Approximately 70.7 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM GSH, 0.5 mM PMSF, 10% Glycerol, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.