1. Recombinant Proteins
  2. Others
  3. CDC37 Protein, Human (sf9, GST)

CDC37 Protein, Human (sf9, GST)

Cat. No.: HY-P75364
Handling Instructions

The CDC37 protein is a co-chaperone that binds multiple kinases and promotes their interaction with the Hsp90 complex, resulting in stability and enhanced activity. CDC37 Protein, Human (sf9, GST) is the recombinant human-derived CDC37 protein, expressed by Sf9 insect cells , with C-GST labeled tag. The total length of CDC37 Protein, Human (sf9, GST) is 378 a.a., with molecular weight of ~70.7 kDa.

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock Quantity
50 μg $650 In-stock
100 μg   Get quote  

* Please select Quantity before adding items.

CDC37 Protein, Human (sf9, GST) Featured Recommendations:

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

The CDC37 protein is a co-chaperone that binds multiple kinases and promotes their interaction with the Hsp90 complex, resulting in stability and enhanced activity. CDC37 Protein, Human (sf9, GST) is the recombinant human-derived CDC37 protein, expressed by Sf9 insect cells , with C-GST labeled tag. The total length of CDC37 Protein, Human (sf9, GST) is 378 a.a., with molecular weight of ~70.7 kDa.

Background

The CDC37 protein serves as a co-chaperone with a pivotal role in facilitating the interaction between numerous kinases and the Hsp90 complex, leading to the stabilization and enhanced activity of these kinases. It functions by inhibiting HSP90AA1 ATPase activity. CDC37 is likely a part of multiple protein complexes, including those containing chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, PPP5C, PTGES3/p23, and client proteins like TSC2, CDK4, AKT, RAF1, and NR3C1. Another complex involving CDC37 comprises chaperones HSP90 and HSP70, co-chaperones PPP5C and TSC1, and client proteins TSC2, CDK4, AKT, RAF1, and NR3C1, but notably lacking STIP1/HOP and PTGES3/p23. CDC37 also forms a distinct complex with Hsp90/HSP90AB1 and CDK6. Its interactions extend to various proteins, including HSP90AA1, AR, CDK4, CDK6, EIF2AK1, RB1, KSR1, and FLCN, FNIP1, and FNIP2. This diverse network of interactions underscores the versatility of CDC37 in regulating the stability and activity of a wide array of kinases and client proteins.

Species

Human

Source

Sf9 insect cells

Tag

C-GST

Accession

Q16543 (M1-V378)

Gene ID
Molecular Construction
N-term
CDC37 (M1-V378)
Accession # Q16543
GST
C-term
Synonyms
Hsp90 co-chaperone Cdc37; CDC37; CDC37A
Molecular Weight

Approximately 70.7 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM GSH, 0.5 mM PMSF, 10% Glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

CDC37 Protein, Human (sf9, GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
CDC37 Protein, Human (sf9, GST)
Cat. No.:
HY-P75364
Quantity:
MCE Japan Authorized Agent: