CDC37 Protein, Human (sf9, GST)
Based on 1 Customer Validation
The CDC37 protein is a co-chaperone that binds multiple kinases and promotes their interaction with the Hsp90 complex, resulting in stability and enhanced activity. CDC37 Protein, Human (sf9, GST) is the recombinant human-derived CDC37 protein, expressed by Sf9 insect cells , with C-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The CDC37 protein is a co-chaperone that binds multiple kinases and promotes their interaction with the Hsp90 complex, resulting in stability and enhanced activity. CDC37 Protein, Human (sf9, GST) is the recombinant human-derived CDC37 protein, expressed by Sf9 insect cells , with C-GST labeled tag.
Background
The CDC37 protein serves as a co-chaperone with a pivotal role in facilitating the interaction between numerous kinases and the Hsp90 complex, leading to the stabilization and enhanced activity of these kinases. It functions by inhibiting HSP90AA1 ATPase activity. CDC37 is likely a part of multiple protein complexes, including those containing chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, PPP5C, PTGES3/p23, and client proteins like TSC2, CDK4, AKT, RAF1, and NR3C1. Another complex involving CDC37 comprises chaperones HSP90 and HSP70, co-chaperones PPP5C and TSC1, and client proteins TSC2, CDK4, AKT, RAF1, and NR3C1, but notably lacking STIP1/HOP and PTGES3/p23. CDC37 also forms a distinct complex with Hsp90/HSP90AB1 and CDK6. Its interactions extend to various proteins, including HSP90AA1, AR, CDK4, CDK6, EIF2AK1, RB1, KSR1, and FLCN, FNIP1, and FNIP2. This diverse network of interactions underscores the versatility of CDC37 in regulating the stability and activity of a wide array of kinases and client proteins.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag C-GST
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Accession
Q16543 (M1-V378)
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Molecular Construction
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N-term
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CDC37 (M1-V378)
Accession # Q16543 -
GST
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C-term
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Protein Length
Full Length
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Synonyms
CDC37; CDC37 Cell Division Cycle 37 Homolog; Cell Division Cycle 37, HSP90 Cochaperone; P50Cdc37; Hsp90 Co-Chaperone Cdc37; CDC37 Cell Division Cycle 37 Homolog (S. Cerevisiae); Hsp90 Chaperone Protein Kinase-Targeting Subunit; Cell Division Cycle 37 Homo
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AA Sequence
MVDYSVWDHIEVSDDEDETHPNIDTASLFRWRHQARVERMEQFQKEKEELDRGCRECKRKVAECQRKLKELEVAEGGKAELERLQAEAQQLRKEERSWEQKLEEMRKKEKSMPWNVDTLSKDGFSKSMVNTKPEKTEEDSEEVREQKHKTFVEKYEKQIKHFGMLRRWDDSQKYLSDNVHLVCEETANYLVIWCIDLEVEEKCALMEQVAHQTIVMQFILELAKSLKVDPRACFRQFFTKIKTADRQYMEGFNDELEAFKERVRGRAKLRIEKAMKEYEEEERKKRLGPGGLDPVEVYESLPEELQKCFDVKDVQMLQDAISKMDPTDAKYHMQRCIDSGLWVPNSKASEAKEGEEAGPGDPLLEAVPKTGDEKDVSV
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Predicted Molecular Mass
70.7 kDa
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Molecular Weight
Approximately 71 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM GSH, 0.5 mM PMSF, 10% Glycerol, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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