FOXO3 Protein, Human (HEK293, His)

The FOXO3 protein is a multifaceted transcriptional activator that regulates apoptosis, autophagy, and post-transcriptional MYC control. It actively regulates autophagy in skeletal muscle and induces autophagy gene expression under starvation conditions. FOXO3 Protein, Human (HEK293, His) is the recombinant human-derived FOXO3 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of FOXO3 Protein, Human (HEK293, His) is 673 a.a., with molecular weight of ~110 kDa.

FOXO3, a transcriptional activator, plays a multifaceted role in cellular processes, including apoptosis and autophagy. It acts as a positive regulator of autophagy in skeletal muscle, activating the expression of autophagy genes under starved conditions. FOXO3 triggers apoptosis in the absence of survival factors, especially during oxidative stress. Additionally, it participates in the post-transcriptional regulation of MYC by inducing the expression of miR-34b and miR-34c, which prevent MYC translation. In response to metabolic stress, FOXO3 translocates into the mitochondria, promoting mtDNA transcription. Furthermore, it regulates chondrogenic commitment of skeletal progenitor cells by promoting SOX9 expression when lipids are scarce. FOXO3 is also a key regulator of regulatory T-cells (Treg) differentiation, activating the expression of FOXP3. In various contexts, FOXO3 forms complexes with SIRT3 and mitochondrial RNA polymerase POLRMT, contributing to its diverse functions. Interactions with proteins such as YWHAB/14-3-3-beta, STK4/MST1, and PIM1, among others, further modulate FOXO3 activity, highlighting its intricate regulatory network.

Human

HEK293

C-10*His

O43524 (M1-G673)

2309

MAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASPAKPSGETAADSMIPEEEDDEDDEDGGGRAGSAMAIGGGGGSGTLGSGLLLEDSARVLAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLPPPQPGAAGGSGQPRKCSSRRNAWGNLSYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKNSIRHNLSLHSRFMRVQNEGTGKSSWWIINPDGGKSGKAPRRRAVSMDNSNKYTKSRGRAAKKKAALQTAPESADDSPSQLSKWPGSPTSRSSDELDAWTDFRSRTNSNASTVSGRLSPIMASTELDEVQDDDAPLSPMLYSSSASLSPSVSKPCTVELPRLTDMAGTMNLNDGLTENLMDDLLDNITLPPSQPSPTGGLMQRSSSFPYTTKGSGLGSPTSSFNSTVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYGNQTLQDLLTSDSLSHSDVMMTQSDPLMSQASTAVSAQNSRRNVMLRNDPMMSFAAQPNQGSLVNQNLLHHQHQTQGALGGSRALSNSVSNMGLSESSSLGSAKHQQQSPVSQSMQTLSDSLSGSSLYSTSANLPVMGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDSLISTQNVVGLNVGNFTGAKQASSQSWVPG

Approximately 110 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from sterile PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.