FOXO3 Protein, Human (HEK293, His)
Based on 1 Customer Validation
The FOXO3 protein is a multifaceted transcriptional activator that regulates apoptosis, autophagy, and post-transcriptional MYC control. It actively regulates autophagy in skeletal muscle and induces autophagy gene expression under starvation conditions. FOXO3 Protein, Human (HEK293, His) is the recombinant human-derived FOXO3 protein, expressed by HEK293 , with C-10*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The FOXO3 protein is a multifaceted transcriptional activator that regulates apoptosis, autophagy, and post-transcriptional MYC control. It actively regulates autophagy in skeletal muscle and induces autophagy gene expression under starvation conditions. FOXO3 Protein, Human (HEK293, His) is the recombinant human-derived FOXO3 protein, expressed by HEK293 , with C-10*His labeled tag.
Background
FOXO3, a transcriptional activator, plays a multifaceted role in cellular processes, including apoptosis and autophagy. It acts as a positive regulator of autophagy in skeletal muscle, activating the expression of autophagy genes under starved conditions. FOXO3 triggers apoptosis in the absence of survival factors, especially during oxidative stress. Additionally, it participates in the post-transcriptional regulation of MYC by inducing the expression of miR-34b and miR-34c, which prevent MYC translation. In response to metabolic stress, FOXO3 translocates into the mitochondria, promoting mtDNA transcription. Furthermore, it regulates chondrogenic commitment of skeletal progenitor cells by promoting SOX9 expression when lipids are scarce. FOXO3 is also a key regulator of regulatory T-cells (Treg) differentiation, activating the expression of FOXP3. In various contexts, FOXO3 forms complexes with SIRT3 and mitochondrial RNA polymerase POLRMT, contributing to its diverse functions. Interactions with proteins such as YWHAB/14-3-3-beta, STK4/MST1, and PIM1, among others, further modulate FOXO3 activity, highlighting its intricate regulatory network.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source HEK293
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Tag C-10*His
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Accession
O43524-1 (M1-G673)
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Gene ID2309
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Molecular Construction
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N-term
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FOXO3 (M1-G673)
Accession # O43524-1 -
10*His
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C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
FOXO3; Forkhead, Drosophila, Homolog Of, In Rhabdomyosarcoma-Like 1; Prev. FKHRL1; Forkhead Box O3A; Prev. FOXO3A; AF6q21 Protein; AF6q21; FOXO3A-Short; FOXO2; FKHRL1P2; Forkhead In Rhabdomyosarcoma-Like 1; FOXO3A-; Forkhead Box Protein O3; Forkhead Box O
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AA Sequence
MAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASPAKPSGETAADSMIPEEEDDEDDEDGGGRAGSAMAIGGGGGSGTLGSGLLLEDSARVLAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLPPPQPGAAGGSGQPRKCSSRRNAWGNLSYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKNSIRHNLSLHSRFMRVQNEGTGKSSWWIINPDGGKSGKAPRRRAVSMDNSNKYTKSRGRAAKKKAALQTAPESADDSPSQLSKWPGSPTSRSSDELDAWTDFRSRTNSNASTVSGRLSPIMASTELDEVQDDDAPLSPMLYSSSASLSPSVSKPCTVELPRLTDMAGTMNLNDGLTENLMDDLLDNITLPPSQPSPTGGLMQRSSSFPYTTKGSGLGSPTSSFNSTVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYGNQTLQDLLTSDSLSHSDVMMTQSDPLMSQASTAVSAQNSRRNVMLRNDPMMSFAAQPNQGSLVNQNLLHHQHQTQGALGGSRALSNSVSNMGLSESSSLGSAKHQQQSPVSQSMQTLSDSLSGSSLYSTSANLPVMGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDSLISTQNVVGLNVGNFTGAKQASSQSWVPG
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Predicted Molecular Mass
71.3 kDa
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Molecular Weight
Approximately 110 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 25 mM Tris-HCl, 100 mM Glycine, pH 7.3, 10% Glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (240 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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