FOXP3 Protein, Human (His)

FOXP3 is a key transcriptional regulator essential for the development and function of regulatory T cells (Tregs), ensuring immune system homeostasis. It acts as a dual repressor and activator, regulating Treg suppressive function and stability while affecting conventional T cell expansion. FOXP3 Protein, Human (His) is the recombinant human-derived FOXP3 protein, expressed by E. coli , with N-His labeled tag. The total length of FOXP3 Protein, Human (His) is 260 a.a., with molecular weight of ~31.7 kDa.

FOXP3, a pivotal transcriptional regulator, is indispensable for the development and inhibitory function of regulatory T-cells (Treg), playing a crucial role in maintaining immune system homeostasis. It acts as both a transcriptional repressor and activator, influencing the Treg lineage's suppressive function and stability, while modulating conventional T-cell expansion and function. FOXP3 coordinates the activation of key genes like CTLA4 and TNFRSF18, concomitant with the repression of cytokine-encoding genes such as interleukin-2 (IL2) and interferon-gamma (IFNG). Through its association with various transcription factors and chromatin modifiers, FOXP3 inhibits cytokine production, T-cell effector function, and the differentiation of IL17-producing helper T-cells (Th17), favoring Treg development. FOXP3's interactions with proteins like IKZF3, RUNX1, RORC, RELA, NFATC2, and others contribute to its diverse regulatory functions. Dimerization is essential for its transcriptional regulator activity, and it forms complexes with proteins such as STUB1, HSPA8, PPP1CA, PPP1CB, KAT5, HDAC7, USP7, ZFP90, TRIM28, RUNX2, RUNX3, and IKZF4.

Human

E. coli

N-His

Q9BZS1 (M1-L260)

50943  [NCBI]

MPNPRPGKPSAPSLALGPSPGASPSWRAAPKASDLLGARGPGGTFQGRDLRGGAHASSSSLNPMPPSQLQLPTLPLVMVAPSGARLGPLPHLQALLQDRPHFMHQLSTVDAHARTPVLQVHPLESPAMISLTPPTTATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPNPSAPRKDSTLSAVPQSSYPLLANGVCKWPGCEKVFEEPEDFLKHCQADHLLDEKGRAQCLLQREMVQSLEQQLVLEKEKLSAMQAHL

Approximately 31.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against solution in Tris-based buffer, 50% glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.