FOXP3 Protein, Human (His)
Based on 1 Customer Validation
FOXP3 is a key transcriptional regulator essential for the development and function of regulatory T cells (Tregs), ensuring immune system homeostasis. It acts as a dual repressor and activator, regulating Treg suppressive function and stability while affecting conventional T cell expansion. FOXP3 Protein, Human (His) is the recombinant human-derived FOXP3 protein, expressed by E. coli , with N-His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
FOXP3 is a key transcriptional regulator essential for the development and function of regulatory T cells (Tregs), ensuring immune system homeostasis. It acts as a dual repressor and activator, regulating Treg suppressive function and stability while affecting conventional T cell expansion. FOXP3 Protein, Human (His) is the recombinant human-derived FOXP3 protein, expressed by E. coli , with N-His labeled tag.
Background
FOXP3, a pivotal transcriptional regulator, is indispensable for the development and inhibitory function of regulatory T-cells (Treg), playing a crucial role in maintaining immune system homeostasis. It acts as both a transcriptional repressor and activator, influencing the Treg lineage's suppressive function and stability, while modulating conventional T-cell expansion and function. FOXP3 coordinates the activation of key genes like CTLA4 and TNFRSF18, concomitant with the repression of cytokine-encoding genes such as interleukin-2 (IL2) and interferon-gamma (IFNG). Through its association with various transcription factors and chromatin modifiers, FOXP3 inhibits cytokine production, T-cell effector function, and the differentiation of IL17-producing helper T-cells (Th17), favoring Treg development. FOXP3's interactions with proteins like IKZF3, RUNX1, RORC, RELA, NFATC2, and others contribute to its diverse regulatory functions. Dimerization is essential for its transcriptional regulator activity, and it forms complexes with proteins such as STUB1, HSPA8, PPP1CA, PPP1CB, KAT5, HDAC7, USP7, ZFP90, TRIM28, RUNX2, RUNX3, and IKZF4.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-His
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Accession
Q9BZS1-1 (M1-L260)
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Molecular Construction
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N-term
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His
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FOXP3 (M1-L260)
Accession # Q9BZS1-1 -
C-term
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Protein Length
Partial
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Synonyms
FOXP3; PIDX; Prev. IPEX; Immune Dysregulation, Polyendocrinopathy, Enteropathy, X-Linked; Forkhead Box Protein P3; SCURFIN; DIETER; Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked; XPID; FOXP3delta7; AIID; Scurfin; Forkhead Box P3; JM2
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AA Sequence
MPNPRPGKPSAPSLALGPSPGASPSWRAAPKASDLLGARGPGGTFQGRDLRGGAHASSSSLNPMPPSQLQLPTLPLVMVAPSGARLGPLPHLQALLQDRPHFMHQLSTVDAHARTPVLQVHPLESPAMISLTPPTTATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPNPSAPRKDSTLSAVPQSSYPLLANGVCKWPGCEKVFEEPEDFLKHCQADHLLDEKGRAQCLLQREMVQSLEQQLVLEKEKLSAMQAHL
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Predicted Molecular Mass
31.7 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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