Galectin-3/LGALS3 Protein, Mouse (HEK293, His)

Galectin-3/LGALS3 protein binds IgE and cooperates with integrins α-3 and β-1 to promote endothelial cell migration. It contributes to epithelial cell differentiation and acts as a splicing factor in the nucleus. Galectin-3/LGALS3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Galectin-3/LGALS3 protein, expressed by HEK293 , with C-His labeled tag. The total length of Galectin-3/LGALS3 Protein, Mouse (HEK293, His) is 263 a.a., with molecular weight of 40-50 kDa.

Galectin-3 (LGALS3) functions as a galactose-specific lectin with the ability to bind IgE and, in collaboration with the alpha-3, beta-1 integrin, participates in the CSPG4-induced stimulation of endothelial cell migration. Alongside DMBT1, it is essential for the terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus, Galectin-3 acts as a pre-mRNA splicing factor. Outside the nucleus, it plays a crucial role in acute inflammatory responses, involving neutrophil activation and adhesion, monocyte macrophage chemoattraction, opsonization of apoptotic neutrophils, and mast cell activation. Furthermore, in coordination with TRIM16, Galectin-3 facilitates the recognition of membrane damage, mobilizing core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes. The protein likely forms homo- or heterodimers and interacts with various partners, including DMBT1, CD6, ALCAM, ITGA3, ITGB1, CSPG4, LGALS3BP, LYPD3, ZFTRAF1, and UACA, with the interaction with TRIM16 mediating the autophagy of damaged endomembranes.

Mouse Galectin-3, His Tag immobilized on CM5 Chip can bind Mouse LGALS3BP, His Tag with an affinity constant of 17.56 nM as determined in SPR assay (Biacore T200).

Mouse

HEK293

C-His

P16110 (A2-I264)

16854  [NCBI]

40-50 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, 300 mM L-Arginine, pH 7.4 or 50 mM HEPES, 150 mM NaCl, 5 mM EDTA, 2 mM TCEP, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.