IFN-gamma R2 Protein, Human (CHO)
Based on 1 Customer Validation
IFN-gamma R2, one of the subunit of IFN-gamma receptor, is a receptor for IFN-gamma. IFN-gamma R2 is the signal-transducing chain of the IFN-gamma receptor. IFN-gamma R2 forms the functional receptor with IFN-gamma R1. Upon binding with IFN-gamma, IFN-gamma R2 and IFN-gamma R1 oligomerize and transphosphorylate. Then, the downstream signaling components JAK1 and JAK2 are phosphorylated and activated, and STAT1 is phosphorylated. Phosphorylated STAT1 translocates to the nucleus, where it regulates the expression of IFN-responsive genes (e.g. CD54). IFN-gamma R2 Protein, Human (CHO) is a recombinant human IFN-gamma R2 (S28-Q247) without any tag, which is produced in CHO cells.
- Species: Human
- Source: CHO
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
IFN-gamma R2, one of the subunit of IFN-gamma receptor, is a receptor for IFN-gamma. IFN-gamma R2 is the signal-transducing chain of the IFN-gamma receptor. IFN-gamma R2 forms the functional receptor with IFN-gamma R1. Upon binding with IFN-gamma, IFN-gamma R2 and IFN-gamma R1 oligomerize and transphosphorylate[1]. Then, the downstream signaling components JAK1 and JAK2 are phosphorylated and activated, and STAT1 is phosphorylated. Phosphorylated STAT1 translocates to the nucleus, where it regulates the expression of IFN-responsive genes (e.g. CD54)[2]. IFN-gamma R2 Protein, Human (CHO) is a recombinant human IFN-gamma R2 (S28-Q247) without any tag, which is produced in CHO cells.
Background
IFN-gamma R2, one of the subunit of IFN-gamma receptor, is a receptor for IFN-gamma. IFN-gamma R2. IFN-gamma R2 is highly expressed on myeloid cells, moderately on B cells and low on T cells, and the expression is regulated by the state of cellular differentiation or activation[2].
IFN-gamma R2 can associate with IFN-gamma R1 to form a functional receptor. Upon binding with IFN-gamma, IFN-gamma R2 and IFN-gamma R1 oligomerize and transphosphorylate[1]. Then, JAK1 and JAK2 are phosphorylated and activated, creating a binding site for STAT1. STAT1 is recruited to the receptor complex and leads to the phosphorylation. Phosphorylated STAT1 translocates to the nucleus, where it regulates the expression of IFN-responsive genes (e.g. CD54). IFN-gamma R2 is important for innating immune defense against mycobacterial infections. Defects in IFN-gamma R2 causes mendelian susceptibility to mycobacterial disease (MSMD)[2].
Human IFN-gamma R2 consists of extracellular domain (S28-Q247), helical domain (V248-F268), and cytoplasmic domain (L269-L337).
IFN-gamma R2 plays a critical role in antimicrobial, and immune responses[2].
Verified Bioactivity
ED50 < 0.1 μg/ml, measured in a cell cytotoxicity assay using HT-29 (HTB-38) cells in the presence of 1.0 ng/ml human IFN-gamma.
Technical Parameters
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Species Human
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Source CHO
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Tag Tag Free
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Accession
P38484 (S28-Q247)
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Molecular Construction
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N-term
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IFN-gamma R2 (S28-Q247)
Accession # P38484 -
C-term
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Protein Length
Extracellular Domain
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Synonyms
IFNGR2; CDNA FLJ78008, Highly Similar To Human Clone PSK1 Interferon Gamma Receptor Accessory Factor-1 (AF-1) MRNA; Prev. IFNGT1; Interferon Gamma Receptor 2 (Interferon Gamma Transducer 1); AF-1; Interferon Gamma Receptor Accessory Factor-1; Interferon G
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AA Sequence
SQLPAPQHPKIRLYNAEQVLSWEPVALSNSTRPVVYQVQFKYTDSKWFTADIMSIGVNCTQITATECDFTAASPSAGFPMDFNVTLRLRAELGALHSAWVTMPWFQHYRNVTVGPPENIEVTPGEGSLIIRFSSPFDIADTSTAFFCYYVHYWEKGGIQQVKGPFRSNSISLDNLKPSRVYCLQVQAQLLWNKSNIFRVGHLSNISCYETMADASTELQQ
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Molecular Weight
Approximately 38-40 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS.
<0.2 EU/μg, determined by gel clotting method
It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Castro F, et al. Interferon-Gamma at the Crossroads of Tumor Immune Surveillance or Evasion. Front Immunol. 2018 May 4;9:847. [Content Brief]
[2]. Pestka S, et al. The interferon gamma (IFN-gamma) receptor: a paradigm for the multichain cytokine receptor. Cytokine Growth Factor Rev. 1997 Sep;8(3):189-206. [Content Brief]
[3]. van de Vosse E, et al. IFN-γR1 defects: Mutation update and description of the IFNGR1 variation database. Hum Mutat. 2017 Oct;38(10):1286-1296. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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