Copy Link Print Information

IL-18 Protein, Human

5 Cited Publications
Customer Review

Based on 5 publication(s) in Google Scholar

IL-18 protein is a pro-inflammatory cytokine that plays a critical role in epithelial barrier repair and polarized immune responses involving Th1 cells and NK cells. After binding to IL18R1 and IL18RAP, it forms a signaling ternary complex that activates NF-kappa-B, leading to the synthesis of inflammatory mediators. IL-18 Protein, Human is the recombinant human-derived IL-18 protein, expressed by E. coli , with tag free.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for IL-18 Protein, Human
  • Experimental Validation Results for IL-18 Protein, Human
  • Experimental Validation Results for IL-18 Protein, Human
  • Experimental Validation Results for IL-18 Protein, Human
  • Experimental Validation Results for IL-18 Protein, Human
  • Experimental Validation Results for IL-18 Protein, Human
  • IL-18 Protein, Human Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

IL-18 protein is a pro-inflammatory cytokine that plays a critical role in epithelial barrier repair and polarized immune responses involving Th1 cells and NK cells. After binding to IL18R1 and IL18RAP, it forms a signaling ternary complex that activates NF-kappa-B, leading to the synthesis of inflammatory mediators. IL-18 Protein, Human is the recombinant human-derived IL-18 protein, expressed by E. coli , with tag free.

Background

IL-18 Protein is a pro-inflammatory cytokine with pivotal roles in epithelial barrier repair and orchestrating polarized immune responses involving T-helper 1 (Th1) cells and natural killer (NK) cells. Upon binding to IL18R1 and IL18RAP, IL-18 forms a signaling ternary complex that activates NF-kappa-B, leading to the synthesis of inflammatory mediators. It synergizes with IL-12 to induce IFNG synthesis in Th1 cells and NK cells. Notably, IL-18 is involved in transduction of inflammation downstream of pyroptosis, where its mature form is released into the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. At the plasma membrane, IL-18 forms a ternary complex with the ligand-binding receptor subunit IL18R1 and signaling receptor subunit IL18RAP, initiating intracellular signaling. Additionally, IL-18 interacts with the cargo receptor TMED10, facilitating its translocation from the cytoplasm into the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and subsequent secretion.

Verified Bioactivity

1.Measured by its ability to induce IFN-gamma secretion by KG-1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is 2-20 ng/mL in the presence of 15 ng/mL TNF-alpha.
2.Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells.The ED50 for this effect is 0.5-2.5 ng/mL.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    Q14116-1 (Y37-D193)

  • Gene ID
    3606  [NCBI]
  • Molecular Construction
    • N-term
    • IL-18 (Y37-D193)
      Accession # Q14116-1
    • C-term
  • Protein Length

    Full Length of Isoform-1 Mature Protein

  • Synonyms

    IL18; Interleukin 18 (Interferon-Gamma-Inducing Factor); Interleukin 18; IFN-Gamma-Inducing Factor; IL-18; Interleukin-1 Gamma; IL1F4; Iboctadekin; IGIF; IL-1 Gamma; Interleukin-18; Interferon-Gamma-Inducing Factor; IL-1g; Interferon Gamma-Inducing Factor

  • AA Sequence

    YFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED

  • Predicted Molecular Mass

    18.2 kDa

  • Molecular Weight

    Approximately 17-19 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
3.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
4.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, 5% sucrose, 0.1% Tween 80, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to induce IFN-gamma secretion by KG-1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is 14 ng/mL in the presence of 15 ng/mL TNF-alpha, corresponding to a specific activity is 7.14×104 U/mg.

  • 1/16

    IL-18 Protein, Human purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Mar 30;26(7):3211. [Abstract]

    Customer Validation
     Changes in the protein expression levels of Gzmb and Prf1 in NC and Thy1-OE NK92 cells following stimulation with IL-12, IL-15, and IL-18 (100 ng/mL) for 24 hours were examined.

  • 1/16

    IL-18 Protein, Human purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Mar 30;26(7):3211. [Abstract]

    Customer Validation
     Changes in the gene expression levels of Gzmb and Prf1 in NC and Thy1-OE NK92 cells following stimulation with IL-12, IL-15, and IL-18 (100 ng/mL) for 24 hours were examined.

  • 1/16

    IL-18 Protein, Human purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Mar 30;26(7):3211. [Abstract]

    Customer Validation
     Changes in Gzmb protein expression levels in NC and Thy1-OE NK92 cells after stimulation with IL-12 (50 ng/mL), IL-15 (50 ng/mL), and IL-18 (100 ng/mL) for 24 hours.

  • 1/16

    IL-18 Protein, Human purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Oct;11(38):e2406633. [Abstract]

    Customer Validation
     Illustration of a subcutaneous tumor in HuPBMC NOG mice. IL-18 Protein inhibited the growth of subcutaneous gallbladder cancer tumors in vivo, whereas ΔIL-18 significantly promoted their growth.

  • IL-18 Protein, Human Product Image
    1/16
  • Experimental Validation Results for IL-18 Protein, Human
  • Experimental Validation Results for IL-18 Protein, Human
  • Experimental Validation Results for IL-18 Protein, Human
  • Experimental Validation Results for IL-18 Protein, Human
  • Experimental Validation Results for IL-18 Protein, Human
  • Experimental Validation Results for IL-18 Protein, Human
  • IL-18 Protein, Human Product Image