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IL-2 Protein, Human

24 Cited Publications
Customer Review

Based on 24 publication(s) in Google Scholar

IL-2 Protein, Human activates STAT, PI3K and MAPK pathways. IL-2 Protein, Human increases NK-cell cytolytic activity as a T-cell growth factor. IL-2 Protein, Human regulates the proliferation of activated B-cells and regulatory T-cells. IL-2 Protein, Human is the recombinant human-derived IL-2 Protein, expressed by E. coli.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for IL-2 Protein, Human
  • Experimental Validation Results for IL-2 Protein, Human
  • IL-2 Protein, Human Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

IL-2 Protein, Human activates STAT, PI3K and MAPK pathways. IL-2 Protein, Human increases NK-cell cytolytic activity as a T-cell growth factor. IL-2 Protein, Human regulates the proliferation of activated B-cells and regulatory T-cells. IL-2 Protein, Human is the recombinant human-derived IL-2 Protein, expressed by E. coli.

Background

Recombinant human interleukin-2 (IL-2) expressed as Escherichia coli is isolated as insoluble aggregates of protein (inclusion bodies) after cell breakage. Interleukin 2 (IL-2), a cytokine secreted by activated T cells, promotes the differentiation and proliferation of lymphoid cells as well as enhances the cell-mediated immune response to infections. Therefore IL-2 has been used as an adjunctive immunotherapeutic agent to control some infectious diseases, such as leishmaniasis, leprosy, and HIV infection[1].

In Vitro

IL-2 Protein (Human, 500 U/mL, 42 hours-5 days) induces cytotoxic activity in mouse splenocytes and human peripheral blood lymphocytes, which exhibits a killing effect on tumor cells[2].

Verified Bioactivity

1. The ED50 as determined by the dose-dependant stimulation of the proliferation of CTLL-2 was found to be < 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg.
2. Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells.The ED50 for this effect is <3.0 ng/mL.

Results
  • Experimental Validation Results for IL-2 Protein, Human
    The ED50 is 0.1 ng/mL as measured by CTLL-2 cells, corresponding to a specific activity of 1 × 107 units/mg.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    P60568 (A21-T153)

  • Gene ID
    3558  [NCBI]
  • Molecular Construction
    • N-term
    • IL-2 (A21-T153)
      Accession # P60568
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    rHuIL-2; IL2; T-cell Growth Factor; TCGF; Aldesleukin

  • AA Sequence

    APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT

  • Predicted Molecular Mass

    16.9 kDa

  • Molecular Weight

    Approximately 14-16 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

    • Experimental Validation Results for IL-2 Protein, Human
      ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 10 mM sodium citrate, pH 4.0.
2.Lyophilized from a 0.22 μm filtered solution of 10 mM sodium citrate, pH 4.0, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Zhang R, et al. Therapeutic effects of recombinant human interleukin 2 as adjunctive immunotherapy against tuberculosis: A systematic review and meta-analysis. PLoS One. 2018 Jul 19;13(7):e0201025. [Content Brief]

[2]. Devos R, et al., Induction of cytolytic cells by pure recombinant human interleukin 2. Eur J Immunol. 1984 Nov;14(11):1057-60. [Content Brief]

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

  • ≥ 95%, as determined by reducing SDS-PAGE.

  • The ED50 is 0.1 ng/mL as measured by CTLL-2 cells, corresponding to a specific activity of 1 × 107 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     The ED50 is 0.1 ng/mL as measured by CTLL-2 cells, corresponding to a specific activity of 1 × 107 units/mg.

  • IL-2 Protein, Human Product Image
    1/16
  • Experimental Validation Results for IL-2 Protein, Human
  • Experimental Validation Results for IL-2 Protein, Human
  • IL-2 Protein, Human Product Image