NGFR Protein, Human (HEK293)
Based on 1 Customer Validation
Nerve Growth Factor Receptor (NGFR) also known as CD271, p75NTR, TNFRSF16, is a transmembrane low-affinity receptor for the neurotrophin family. NGFR has no intrinsic tyrosine kinase activity. NGFR can act as a tumour suppressor, negatively regulating cell growth and proliferation. NGF-NGFR cascade activates NF-κB, leading to inhibition of apoptosis, increases survival of schwannoma, and breast cancer cells. NGFR Protein, Human (HEK293) is a recombinant protein consisting of 222 amino acids (K29-N250) and is produced in HEK293 cells.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Nerve Growth Factor Receptor (NGFR) also known as CD271, p75NTR, TNFRSF16, is a transmembrane low-affinity receptor for the neurotrophin family. NGFR has no intrinsic tyrosine kinase activity. NGFR can act as a tumour suppressor, negatively regulating cell growth and proliferation[1]. NGF-NGFR cascade activates NF-κB, leading to inhibition of apoptosis, increases survival of schwannoma, and breast cancer cells[3]. NGFR Protein, Human (HEK293) is a recombinant protein consisting of 222 amino acids (K29-N250) and is produced in HEK293 cells.
Nerve Growth Factor Receptor (NGFR) is expressed not only in nervous tissue, but also in non-neuronal normal and cancer cells, such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells and myoepithelial cells[1].
Human NGFR shares 92.45% aa sequence identity with mouse NGFR protein and 92.42% aa sequence identity with rat NGFR protein.
Nerve Growth Factor Receptor (NGFR) is a type-I transmembrane protein, a typical structure of the TNFR superfamily and devoid of intrinsic catalytic activity. NGFR signaling involves activation of NF-kB (Rel/NF-kB transcription factors) and the phosphorylation of the transcription factor c-Jun kinase (JNK), as well as increased production of ceramide, leading to gene transcription or programmed cell death[2].
NGFR induces p53-dependent apoptosis and cell growth arrest as well as suppressed tumor growth[3]. The low-affinity nerve growth factor receptor (NGFR) p75NGFR induces apoptosis in the absence of nerve growth factor (NGF) binding but enhances neural survival when bound by NGF. NGFR enhances beta-amyloid peptide toxicity[4]. NGFR signal can induce the subsequent downregulation of melanoma antigens and eventually suppress CTL activation[5].
NGFR (human) knockdown induces apoptosis and inhibits p53 activation in H460 cells[3].
NGFR (human) high expression is correlated with greater regional nodal metastasis rates and poor prognosis in human oral squamous cell carcinoma[6].
Measured by its ability to inhibit beta-NGF-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is ≤0.58 µg/mL.
Technical Parameters
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Species Human
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Source HEK293
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Tag Tag Free
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Accession
P08138-1 (K29-N250)
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Molecular Construction
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N-term
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NGFR (K29-N250)
Accession # P08138-1 -
C-term
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Protein Length
Extracellular Domain
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Synonyms
NGFR; Low Affinity Neurotrophin Receptor P75NTR; Nerve Growth Factor Receptor; TNFR Superfamily, Member 16; TNFRSF16; NGF Receptor; P75NTR; Gp80-LNGFR; CD271; P75 ICD; Tumor Necrosis Factor Receptor Superfamily Member 16; Nerve Growth Factor Receptor (TNF
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AA Sequence
KEACPTGLYTHSGECCKACNLGEGVAQPCGANQTVCEPCLDSVTFSDVVSATEPCKPCTECVGLQSMSAPCVEADDAVCRCAYGYYQDETTGRCEACRVCEAGSGLVFSCQDKQNTVCEECPDGTYSDEANHVDPCLPCTVCEDTERQLRECTRWADAECEEIPGRWITRSTPPEGSDSTAPSTQEPEAPPEQDLIASTVAGVVTTVMGSSQPVVTRGTTDN
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Molecular Weight
Approximately 42-58 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of PBS.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.
<0.2 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (265 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Reis-Filho JS, et al. Distribution and significance of nerve growth factor receptor (NGFR/p75NTR) in normal, benign and malignant breast tissue. Mod Pathol. 2006 Feb;19(2):307-19. [Content Brief]
[2]. Chen C, et al. ESM1 mediates NGFR-induced invasion and metastasis in murine oral squamous cell carcinoma. Oncotarget. 2016 Oct 25;7(43):70738-70749. [Content Brief]
[3]. Micera A, et al. Nerve growth factor and tissue repair remodeling: trkA(NGFR) and p75(NTR), two receptors one fate. Cytokine Growth Factor Rev. 2007 Jun-Aug;18(3-4):245-56. [Content Brief]
[4]. Zhou X, et al. Nerve growth factor receptor negates the tumor suppressor p53 as a feedback regulator. Elife. 2016 Jun 10;5:e15099. [Content Brief]
[5]. Rabizadeh S, et al. Expression of the low-affinity nerve growth factor receptor enhances beta-amyloid peptide toxicity. Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10703-6. [Content Brief]
[6]. Furuta J, et al. CD271 on melanoma cell is an IFN-γ-inducible immunosuppressive factor that mediates downregulation of melanoma antigens. J Invest Dermatol. 2014 May;134(5):1369-1377. [Content Brief]
[7]. Chung MK, et al. CD271 Confers an Invasive and Metastatic Phenotype of Head and Neck Squamous Cell Carcinoma through the Upregulation of Slug. Clin Cancer Res. 2018 Feb 1;24(3):674-683. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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