PARP14 Protein, Human
The PARP14 protein is an ADP-ribosyltransferase that uniquely mono-ADP-ribosylates glutamic acid residues on target proteins such as STAT1 and STAT6, unlike PARP1 and PARP2. It catalyzes STAT1 mono-ADP ribosylation at “Glu-657” and “Glu-705”, reduces STAT1 phosphorylation and inhibits the production of pro-inflammatory cytokines in macrophages following IFNG stimulation. PARP14 Protein, Human is the recombinant human-derived PARP14 protein, expressed by E. coli , with tag free.
- Species: Human
- Source: E. coli
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Biological Activity
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Product Properties
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Biological Activity
The PARP14 protein is an ADP-ribosyltransferase that uniquely mono-ADP-ribosylates glutamic acid residues on target proteins such as STAT1 and STAT6, unlike PARP1 and PARP2. It catalyzes STAT1 mono-ADP ribosylation at “Glu-657” and “Glu-705”, reduces STAT1 phosphorylation and inhibits the production of pro-inflammatory cytokines in macrophages following IFNG stimulation. PARP14 Protein, Human is the recombinant human-derived PARP14 protein, expressed by E. coli , with tag free.
PARP14 Protein, an ADP-ribosyltransferase, facilitates the mono-ADP-ribosylation of glutamate residues on target proteins, distinguishing itself from PARP1 and PARP2 by its inability to mediate poly-ADP-ribosylation. Specifically, it has been shown to catalyze the mono-ADP-ribosylation of STAT1 at 'Glu-657' and 'Glu-705', leading to decreased STAT1 phosphorylation and subsequent negative regulation of pro-inflammatory cytokine production in macrophages following IFNG stimulation. However, the exact role of ADP-ribosylation in preventing STAT1 phosphorylation has been debated, with suggestions that the inhibition may be a result of STAT1 'Lys-703' sumoylation. Additionally, PARP14 mono-ADP-ribosylates STAT6, enhancing STAT6-dependent transcription, and in macrophages, it positively regulates MRC1 expression in response to IL4 stimulation by promoting STAT6 phosphorylation. Furthermore, PARP14 engages in mono-ADP-ribosylation of PARP9.
Technical Parameters
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Species Human
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Source E. coli
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Tag Tag Free
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Accession
Q460N5 (F1208-E1387)
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Gene ID54625
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Molecular Construction
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N-term
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PARP14 (F1208-E1387)
Accession # Q460N5 -
C-term
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Protein Length
Partial
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Synonyms
PARP14; Protein mono-ADP-ribosyltransferase PARP14; ADP-ribosyltransferase diphtheria toxin-like 8; ARTD8; B aggressive lymphoma protein 2; Poly [ADP-ribose] polymerase 14; PARP-14
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AA Sequence
FYGTVSSPDSGVYEMKIGSIIFQVASGDITKEEADVIVNSTSNSFNLKAGVSKAILECAGQNVERECSQQAQQRKNDYIITGGGFLRCKNIIHVIGGNDVKSSVSSVLQECEKKNYSSICLPAIGTGNAKQHPDKVAEAIIDAIEDFVQKGSAQSVKKVKVVIFLPQVLDVFYANMKKRE
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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