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PCSK9 Protein, Mouse (HEK293, His, solution)

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The PCSK9 protein regulates cholesterol levels by degrading LDLR, VLDLR, LRP1/APOER, and LRP8/APOER2 receptors. It promotes LDLR degradation, inhibits recycling, induces ubiquitination, and affects APOB degradation. Additionally, PCSK9 influences BACE1 intermediates, ENaC surface expression, and neuronal apoptosis by modulating LRP8/APOER2 levels and anti-apoptotic signaling pathways. PCSK9 Protein, Mouse (HEK293, His, solution) is the recombinant mouse-derived PCSK9 protein, expressed by HEK293, with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for PCSK9 Protein, Mouse (HEK293, His, solution)
  • Experimental Validation Results for PCSK9 Protein, Mouse (HEK293, His, solution)
  • PCSK9 Protein, Mouse (HEK293, His, solution) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

The PCSK9 protein regulates cholesterol levels by degrading LDLR, VLDLR, LRP1/APOER, and LRP8/APOER2 receptors. It promotes LDLR degradation, inhibits recycling, induces ubiquitination, and affects APOB degradation. Additionally, PCSK9 influences BACE1 intermediates, ENaC surface expression, and neuronal apoptosis by modulating LRP8/APOER2 levels and anti-apoptotic signaling pathways. PCSK9 Protein, Mouse (HEK293, His, solution) is the recombinant mouse-derived PCSK9 protein, expressed by HEK293, with C-6*His labeled tag.

Background

The PCSK9 protein plays a pivotal role in the intricate regulation of plasma cholesterol homeostasis. It engages with members of the low-density lipid receptor family, including low-density lipoprotein receptor (LDLR), very low-density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER), and apolipoprotein receptor 2 (LRP8/APOER2), facilitating their degradation within intracellular acidic compartments. Operating through a non-proteolytic mechanism, PCSK9 enhances the degradation of hepatic LDLR via a clathrin LDLRAP1/ARH-mediated pathway and may impede LDLR recycling from endosomes to the cell surface, directing it to lysosomes for degradation. Moreover, PCSK9 induces ubiquitination of LDLR, leading to subsequent degradation, and inhibits the intracellular degradation of APOB through the autophagosome/lysosome pathway, independent of LDLR. Additionally, PCSK9 is implicated in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway, hinders epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by increasing its proteasomal degradation, and modulates neuronal apoptosis by regulating LRP8/APOER2 levels and related anti-apoptotic signaling pathways.

Verified Bioactivity

1.Measured by its binding ability in a functional ELISA. When Recombinant Mouse LDLR Protein is immobilized at 2 µg/mL (100 µL/well) can bind Mouse PCSK9 Protein. The ED50 for this effect is 145.3 ng/mL.
2.Immobilized Recombinant Mouse PCSK9-His at 5 μg/mL (100 μL/well) can bind Biotinylated Human LDL R-His-Avi. The ED50 of Biotinylated Human LDL R-His-Avi is 70-300 ng/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q80W65 (Q35-Q155&S156-Q694)

  • Gene ID
    100102  [NCBI]
  • Molecular Construction
    • N-term
    • PCSK9 (Q35-Q155&S156-Q694)
      Accession # Q80W65
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein (with Propeptide)

  • Synonyms

    PCSK9; PC9; Prev. HCHOLA3; Hypercholesterolemia, Autosomal Dominant 3; NARC-1; Neural Apoptosis Regulated Convertase 1; Subtilisin/Kexin-Like Protease PC9; Neural Apoptosis-Regulated Convertase 1; FH3; LDLCQ1; Proprotein Convertase 9; FHCL3; NARC1; Proprotein Convertase Subtilisin/Kexin Type 9; Convertase Subtilisin/Kexin Type 9 Preproprotein

  • AA Sequence

    QDEDGDYEELMLALPSQEDGLADEAAHVATATFRRCSKEAWRLPGTYIVVLMEETQRLQIEQTAHRLQTRAARRGYVIKVLHIFYDLFPGFLVKMSSDLLGLALKLPHVEYIEEDSFVFAQSIPWNLERIIPAWHQTEEDRSPDGSSQVEVYLLDTSIQGAHREIEGRVTITDFNSVPEEDGTRFHRQASKCDSHGTHLAGVVSGRDAGVAKGTSLHSLRVLNCQGKGTVSGTLIGLEFIRKSQLIQPSGPLVVLLPLAGGYSRILNAACRHLARTGVVLVAAAGNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGKDIIGASSDCSTCFMSQSGTSQAAAHVAGIVARMLSREPTLTLAELRQRLIHFSTKDVINMAWFPEDQQVLTPNLVATLPPSTHETGGQLLCRTVWSAHSGPTRTATATARCAPEEELLSCSSFSRSGRRRGDWIEAIGGQQVCKALNAFGGEGVYAVARCCLVPRANCSIHNTPAARAGLETHVHCHQKDHVLTGCSFHWEVEDLSVRRQPALRSRRQPGQCVGHQAASVYASCCHAPGLECKIKEHGISGPSEQVTVACEAGWTLTGCNVLPGASLTLGAYSVDNLCVARVHDTARADRTSGEATVAAAICCRSRPSAKASWVQ

  • Predicted Molecular Mass

    14 kDa & 58.2 kDa

  • Molecular Weight

    Approximately 19 kDa & 60 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 20% Glycerol, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When Recombinant Mouse LDLR Protein is immobilized at 2 µg/mL (100 µL/well) can bind Mouse PCSK9 Protein. The ED50 for this effect is 145.3 ng/mL.

  • PCSK9 Protein, Mouse (HEK293, His, solution) Product Image
    1/16
  • Experimental Validation Results for PCSK9 Protein, Mouse (HEK293, His, solution)
  • Experimental Validation Results for PCSK9 Protein, Mouse (HEK293, His, solution)
  • PCSK9 Protein, Mouse (HEK293, His, solution) Product Image