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PCSK9 Protein, Rhesus Macaque (HEK293, His)

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Based on 1 Customer Validation

PCSK9 protein regulates cholesterol levels by degrading LDLR, VLDLR, LRP1/APOER and LRP8/APOER2 receptors. It promotes LDLR degradation through non-proteolytic mechanisms, disrupts LDLR recycling, induces LDLR ubiquitination, and inhibits APOB degradation. PCSK9 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived PCSK9 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rhesus Macaque
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for PCSK9 Protein, Rhesus Macaque (HEK293, His)
  • Experimental Validation Results for PCSK9 Protein, Rhesus Macaque (HEK293, His)
  • PCSK9 Protein, Rhesus Macaque (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

PCSK9 protein regulates cholesterol levels by degrading LDLR, VLDLR, LRP1/APOER and LRP8/APOER2 receptors. It promotes LDLR degradation through non-proteolytic mechanisms, disrupts LDLR recycling, induces LDLR ubiquitination, and inhibits APOB degradation. PCSK9 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived PCSK9 protein, expressed by HEK293 , with C-His labeled tag.

Background

PCSK9 Protein plays a crucial role in maintaining the balance of cholesterol levels in the blood. It binds to several receptors involved in lipid metabolism, including LDLR, VLDLR, LRP1/APOER, and LRP8/APOER2, and promotes their degradation in acidic compartments inside cells. It enhances the degradation of hepatic LDLR through a non-proteolytic mechanism mediated by clathrin LDLRAP1/ARH pathway. Additionally, it may disrupt the recycling of LDLR, directing it to lysosomes for degradation. PCSK9 can also induce ubiquitination of LDLR, leading to its subsequent breakdown. Furthermore, it inhibits the degradation of APOB, an important component of lipoproteins, through the autophagosome/lysosome pathway, independently of LDLR. In the early secretory pathway, PCSK9 helps dispose of non-acetylated intermediates of BACE1. It also plays a role in regulating the absorption of sodium ions through the ENaC channel by reducing its surface expression through increased proteasomal degradation. Finally, PCSK9 regulates neuronal apoptosis by modulating the levels of LRP8/APOER2 and related anti-apoptotic signaling pathways.

Verified Bioactivity

Immobilized Rhesus PCSK9 at 5 µg/mL (100 µL/well) can bind Biotinylated rmLDLR. The ED50 for this effect is 224.2 ng/mL

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Immobilized Rhesus PCSK9 at 5 µg/mL (100 µL/well) can bind Biotinylated rmLDLR. The ED50 for this effect is 224.2 ng/mL.

Technical Parameters

  • Species Rhesus Macaque
  • Source HEK293
  • Tag C-His
  • Accession

    A8T666 (Q31-Q692)

  • Gene ID
    717147  [NCBI]
  • Molecular Construction
    • N-term
    • PCSK9 (Q31-Q692)
      Accession # A8T666
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein (with Propeptide)

  • Synonyms

    PCSK9; PC9; Prev. HCHOLA3; Hypercholesterolemia, Autosomal Dominant 3; NARC-1; Neural Apoptosis Regulated Convertase 1; Subtilisin/Kexin-Like Protease PC9; Neural Apoptosis-Regulated Convertase 1; FH3; LDLCQ1; Proprotein Convertase 9; FHCL3; NARC1; Propro

  • AA Sequence

    QEDEDGDYEELVLALRSEEDGLADAPEHGATATFHRCAKDPWRLPGTYVVVLKEETHRSQSERTARRLQAQAARRGYLTKILHVFHHLLPGFLVKMSGDLLELALKLPHVDYIEEDSSVFAQSIPWNLERITPARYRADEYQPPKGGSLVEVYLLDTSIQSDHREIEGRVMVTDFESVPEEDGTRFHRQASKCDSHGTHLAGVVSGRDAGVAKGAGLRSLRVLNCQGKGTVSGTLIGLEFIRKSQLVQPVGPLVVLLPLAGGYSRVFNAACQRLARAGVVLVTAAGNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGEDIIGASSDCSTCFVSRSGTSQAAAHVAGIAAMMLSAEPELTLAELRQRLIHFSAKDVINEAWFPEDQRVLTPNLVAALPPSTHRAGWQLFCRTVWSAHSGPTRMATAVARCAQDEELLSCSSFSRSGKRRGERIEAQGGKRVCRAHNAFGGEGVYAIARCCLLPQVNCSVHTAPPAGASMGTRVHCHQQGHVLTGCSSHWEVEDLGTHKPPVLRPRGQPNQCVGHREASIHASCCHAPGLECKVKEHGIPAPQEQVIVACEDGWTLTGCSPLPGTSHVLGAYAVDNTCVVRSRDVSTTGSTSKEAVAAVAICCRSRHLVQASQELQ

  • Molecular Weight

    Approximately 17-20 kDa (pro domain) & 60-66 kDa (mature form), based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Immobilized Rhesus PCSK9 at 5 µg/mL (100 µL/well) can bind Biotinylated rmLDLR. The ED50 for this effect is 224.2 ng/mL.

  • PCSK9 Protein, Rhesus Macaque (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for PCSK9 Protein, Rhesus Macaque (HEK293, His)
  • Experimental Validation Results for PCSK9 Protein, Rhesus Macaque (HEK293, His)
  • PCSK9 Protein, Rhesus Macaque (HEK293, His) Product Image