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Siglec-3/CD33 Protein, Human (HEK293)

Customer Review

Based on 1 Customer Validation

Siglec-3/CD33, a sialic-acid-binding lectin, crucially mediates cell-cell interactions and immune cell quiescence. Preferring sialic acid on specific mucins, it forms homodimers, interacting with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. CD33 also engages C1QA through its C-terminus, activating CD33 inhibitory motifs. Siglec-3/CD33 Protein, Human (HEK293) is the recombinant human-derived Siglec-3/CD33 protein, expressed by HEK293 , with tag free.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for Siglec-3/CD33 Protein, Human (HEK293)
  • Siglec-3/CD33 Protein, Human (HEK293) Product Image
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Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

Siglec-3/CD33, a sialic-acid-binding lectin, crucially mediates cell-cell interactions and immune cell quiescence. Preferring sialic acid on specific mucins, it forms homodimers, interacting with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. CD33 also engages C1QA through its C-terminus, activating CD33 inhibitory motifs. Siglec-3/CD33 Protein, Human (HEK293) is the recombinant human-derived Siglec-3/CD33 protein, expressed by HEK293 , with tag free.

Background

Siglec-3/CD33, a sialic-acid-binding immunoglobulin-like lectin, plays a crucial role in mediating cell-cell interactions and maintaining immune cells in a resting state. It exhibits a preference for binding sialic acid on the short O-linked glycans of specific mucins. The protein forms homodimers through disulfide linkages and interacts with signaling molecules such as PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. Additionally, CD33 engages with C1QA via its C-terminus, leading to the activation of CD33 inhibitory motifs.

Verified Bioactivity

1.Immobilized Recombinant Human CD33 / Siglec-3 Proteinat 2 μg/mL (100 μL/well) can bind Anti-CD33 Antibody (Lintuzumab), Human IgG1, the EC50 is 5.5 ng/mL.
2.Loaded Anti-CD33 Antibody (OR000283) (HY-P9S0124) on ACH2 biosensor, can bind Siglec-3/CD33 Protein, Human (HEK293) with an affinity constant of 8.988E-10 M as determined in BLI assay.

MCE Validation Data

  • Bioactivity - BLI

    Bioactivity - BLI

    Loaded Anti-CD33 Antibody (OR000283) (HY-P9S0124) on ACH2 biosensor, can bind Siglec-3/CD33 Protein, Human (HEK293) with an affinity constant of 8.988E-10 M as determined in BLI assay.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag Tag Free
  • Accession

    AAH28152 (D18-H259)

  • Gene ID
    945  [NCBI]
  • Molecular Construction
    • N-term
    • CD33 (D18-H259)
      Accession # AAH28152
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    CD33; Myeloid Cell Surface Antigen CD33; CD33 Molecule; FLJ00391; SIGLEC3; Gp67; CD33 Antigen (Gp67); CDNA FLJ50122, Highly Similar To Myeloid Cell Surface Antigen CD33; CD33rSiglec; Sialic Acid Binding Ig-Like Lectin 3; SIGLEC-3; CD33 Molecule Transcript

  • AA Sequence

    DPNFWLQVQESVTVQEGLCVLVPCTFFHPIPYYDKNSPVHGYWFREGAIISGDSPVATNKLDQEVQEETQGRFRLLGDPSRNNCSLSIVDARRRDNGSYFFRMERGSTKYSYKSPQLSVHVTDLTHRPKILIPGTLEPGHSKNLTCSVSWACEQGTPPIFSWLSAAPTSLGPRTTHSSVLIITPRPQDHGTNLTCQVKFAGAGVTTERTIQLNVTYVPQNPTTGIFPGDGSGKQETRAGVVH

  • Predicted Molecular Mass

    27.4 kDa

  • Molecular Weight

    Approximately 45 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

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MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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    MedChemExpress Validation
     Loaded Anti-CD33 Antibody (OR000283) (HY-P9S0124) on ACH2 biosensor, can bind Siglec-3/CD33 Protein, Human (HEK293) with an affinity constant of 8.988E-10 M as determined in BLI assay.

  • Siglec-3/CD33 Protein, Human (HEK293) Product Image
    1/16
  • Experimental Validation Results for Siglec-3/CD33 Protein, Human (HEK293)
  • Siglec-3/CD33 Protein, Human (HEK293) Product Image