Toll-like receptor 8/TLR8 Protein, Human (P.pastoris, His)
Based on 1 Customer Validation
The TLR8 protein is an endosomal receptor critical for innate and adaptive immunity. Toll-like receptor 8/TLR8 Protein, Human (P.pastoris, His) is the recombinant human-derived Toll-like receptor 8/TLR8 protein, expressed by P. pastoris , with N-6*His labeled tag.
- Species: Human
- Source: P. pastoris
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The TLR8 protein is an endosomal receptor critical for innate and adaptive immunity. Toll-like receptor 8/TLR8 Protein, Human (P.pastoris, His) is the recombinant human-derived Toll-like receptor 8/TLR8 protein, expressed by P. pastoris , with N-6*His labeled tag.
Background
Toll-like receptor 8 (TLR8) Protein, an endosomal receptor central to innate and adaptive immunity, plays a crucial role in orchestrating the host immune response against pathogens by recognizing RNA degradation products specific to microorganisms, initially processed by RNASET2. Notably, TLR8 is adept at recognizing GU-rich single-stranded RNA (GU-rich RNA) derived from various viruses, including SARS-CoV-2, SARS-CoV-1, and HIV-1. Upon binding to agonists, TLR8 undergoes dimerization, facilitating the direct contact of Toll/Interleukin-1 receptor (TIR) domains, leading to the recruitment of the downstream adapter MYD88 through homotypic interaction. This sets off the formation of the Myddosome signaling complex involving IRAK4, IRAK1, TRAF6, and TRAF3, ultimately activating downstream transcription factors NF-kappa-B and IRF7 to induce pro-inflammatory cytokines and interferons. TLR8's activation is particularly triggered by RNAs containing a sufficient number of uridines, underscoring its specificity in pathogenic RNA recognition and immune response initiation.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source P. pastoris
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Tag N-6*His
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Accession
Q9NR97 (E27-T827)
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Molecular Construction
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N-term
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6*His
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TLR8 (E27-T827)
Accession # Q9NR97 -
C-term
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Protein Length
Extracellular Domain
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Synonyms
TLR8; Toll-Like Receptor 8 Transcript Variant 4; Toll Like Receptor 8; CD288 Antigen; Toll-Like Receptor 8; IMD98; CD288; TLR-8; HTLR8
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AA Sequence
EENFSRSYPCDEKKQNDSVIAECSNRRLQEVPQTVGKYVTELDLSDNFITHITNESFQGLQNLTKINLNHNPNVQHQNGNPGIQSNGLNITDGAFLNLKNLRELLLEDNQLPQIPSGLPESLTELSLIQNNIYNITKEGISRLINLKNLYLAWNCYFNKVCEKTNIEDGVFETLTNLELLSLSFNSLSHVPPKLPSSLRKLFLSNTQIKYISEEDFKGLINLTLLDLSGNCPRCFNAPFPCVPCDGGASINIDRFAFQNLTQLRYLNLSSTSLRKINAAWFKNMPHLKVLDLEFNYLVGEIASGAFLTMLPRLEILDLSFNYIKGSYPQHINISRNFSKLLSLRALHLRGYVFQELREDDFQPLMQLPNLSTINLGINFIKQIDFKLFQNFSNLEIIYLSENRISPLVKDTRQSYANSSSFQRHIRKRRSTDFEFDPHSNFYHFTRPLIKPQCAAYGKALDLSLNSIFFIGPNQFENLPDIACLNLSANSNAQVLSGTEFSAIPHVKYLDLTNNRLDFDNASALTELSDLEVLDLSYNSHYFRIAGVTHHLEFIQNFTNLKVLNLSHNNIYTLTDKYNLESKSLVELVFSGNRLDILWNDDDNRYISIFKGLKNLTRLDLSLNRLKHIPNEAFLNLPASLTELHINDNMLKFFNWTLLQQFPRLELLDLRGNKLLFLTDSLSDFTSSLRTLLLSHNRISHLPSGFLSEVSSLKHLDLSSNLLKTINKSALETKTTTKLSMLELHGNPFECTCDIGDFRRWMDEHLNVKIPRLVDVICASPGDQRGKSIVSLELTTCVSDVT
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Predicted Molecular Mass
93.5 kDa
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Molecular Weight
Approximately 93-100 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HC1, 0.5 M NaCl, 3% trehalose, pH 8.0.
2.Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (240 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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