1. Recombinant Proteins
  2. Ubiquitin Related Proteins
  3. Deubiquitinase
  4. UCH Proteins
  5. UCH-L1
  6. UCHL1 Protein, Mouse (His)

UCHL1 Protein, Mouse (His)

Cat. No.: HY-P73466
Handling Instructions

UCHL1 Protein, a ubiquitin carboxyl-terminal hydrolase, plays a crucial role in protein degradation and regulation of cellular processes. It is involved in maintaining neuronal integrity and has been linked to neurodegenerative diseases. UCHL1 Protein's potential as a biomarker and therapeutic target in neurodegenerative disorders makes it a subject of intense research in the field of neuroscience. UCHL1 Protein, Mouse (His) is the recombinant mouse-derived UCHL1 protein, expressed by E. coli , with N-His labeled tag. The total length of UCHL1 Protein, Mouse (His) is 222 a.a., with molecular weight of ~29 kDa.

For research use only. We do not sell to patients.

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Description

UCHL1 Protein, a ubiquitin carboxyl-terminal hydrolase, plays a crucial role in protein degradation and regulation of cellular processes. It is involved in maintaining neuronal integrity and has been linked to neurodegenerative diseases. UCHL1 Protein's potential as a biomarker and therapeutic target in neurodegenerative disorders makes it a subject of intense research in the field of neuroscience. UCHL1 Protein, Mouse (His) is the recombinant mouse-derived UCHL1 protein, expressed by E. coli , with N-His labeled tag. The total length of UCHL1 Protein, Mouse (His) is 222 a.a., with molecular weight of ~29 kDa.

Background

UCHL1 Protein is a deubiquitinase that plays a crucial role in various processes, including maintenance of synaptic function, cardiac function, inflammatory response, and osteoclastogenesis. It inhibits the ubiquitination of several proteins, such as WWTR1/TAZ, EGFR, HIF1A, and beta-site amyloid precursor protein cleaving enzyme 1/BACE1. Additionally, it recognizes and hydrolyzes the peptide bond at the C-terminal glycine of ubiquitin, which helps maintain a stable pool of monoubiquitin necessary for the ubiquitin-proteasome and autophagy-lysosome pathways. UCHL1 Protein also regulates amyloid precursor protein/APP processing by promoting BACE1 degradation, leading to reduced amyloid beta production. It is involved in the immune response by regulating the ability of MHC I molecules to reach compartments essential for generating Ag-MHC I complexes. Furthermore, UCHL1 Protein mediates the deubiquitination of the transcriptional coactivator WWTR1/TAZ, stabilizing it and inhibiting osteoclastogenesis. It also stabilizes epidermal growth factor receptor EGFR, preventing its degradation and activating downstream mediators. Moreover, UCHL1 Protein modulates oxidative activity in skeletal muscle by regulating key mitochondrial oxidative proteins. Lastly, it enhances the activity of hypoxia-inducible factor 1-alpha/HIF1A by inhibiting its degradation through abrogating its VHL E3 ligase-mediated ubiquitination.

Species

Mouse

Source

E. coli

Tag

N-His

Accession

Q9R0P9 (Q2-A223)

Gene ID

22223  [NCBI]

Molecular Construction
N-term
His
UCHL1 (Q2-A223)
Accession # Q9R0P9
C-term
Synonyms
Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; Uchl1; PGP9.5
Molecular Weight

Approximately 29 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

UCHL1 Protein, Mouse (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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UCHL1 Protein, Mouse (His)
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HY-P73466
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