Sphingomyelin phosphodiesterase, Bacillus cereus
Sphingomyelin phosphodiesterase, Bacillus cereus is a sphingomyelin hydrolase and Insecticide. Sphingomyelin phosphodiesterase, Bacillus cereus catalyzes the decomposition of sphingomyelin into ceramide and phosphorylcholine. Sphingomyelin phosphodiesterase, Bacillus cereus causes rapid paralysis and death in injected insects, including German cockroaches and black cutworms. Sphingomyelin phosphodiesterase, Bacillus cereus reduces H2O2 production. Sphingomyelin phosphodiesterase, Bacillus cereus can be used in studies related to sepsis and endophthalmitis.
For research use only. We do not sell to patients.
- CAS No.: 9031-54-3
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Sphingomyelin phosphodiesterase from *Bacillus cereus* (0-200 μM; 30 min) hydrolyzes sphingomyelin in a dose-dependent manner, with a dissociation constant (Kd) of approximately 50 μM[1].
Sphingomyelin phosphodiesterase from *Bacillus cereus* (0.1-1.0 μg/mL; 60 min pre-incubation) dose-dependently inhibits peptidoglycan-induced H2O2 production and phagocytosis in mouse peritoneal macrophages, without altering TNF-α release or inducing cytotoxicity[3].
Sphingomyelin phosphodiesterase, Bacillus cereus (0.05-1.0 μg/mL; 60 min) dose-dependently increases ceramide levels in mouse peritoneal macrophages, induces the formation of ceramide-enriched membrane domains, and reduces membrane fluidity[3].
Sphingomyelin phosphodiesterase from *Bacillus cereus* (30 min) exhibits hemolytic activity against sheep red blood cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Recombinant *Bacillus cereus* sphingomyelin phosphodiesterase (administered via lateral injection, single dose) exhibits potent acute insecticidal activity against black cutworm larvae, with an MPD of 110 ng per insect; whereas inactivation of sphingomyelinase activity via the His151Ala mutation completely abolishes this toxicity[1].
Sphingomyelin phosphodiesterase from *Bacillus cereus* (0.1-5.0 μg per mouse; single dose) dose-dependently enhances the virulence of non-pathogenic B. cereus ATCC21928 in mice. At a dose of 5.0 μg per mouse, it induces 100% mortality, with a blood bacterial count of 300-400 CFU/100 μl[3].
Sphingomyelin phosphodiesterase, *Bacillus cereus* (a mixture of 25 μg Sphingomyelin phosphodiesterase, *Bacillus cereus* and complete Freund's adjuvant; administered subcutaneously; once every two weeks for a total of 2 times) provides complete protection to BALB/c mice against challenge with a lethal clinical isolate of B. cereus, resulting in a 100% survival rate of the mice[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:larvae[1]
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Dosage:110 ng per insect (recombinant); >635 ng per insect (His151Ala mutant)
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Administration:lateral injection; single dose
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Result:Exhibited an MPD of 110 ng per insect (n=3).
Exhibited an MPD of >635 ng per insect (n=3), with no measurable acute insecticidal activity at tested doses.
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Animal Model:ICR mice (6-8-week-old male; septicemia model via intraperitoneal challenge with non-pathogenic B. cereus ATCC21928)[3]
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Dosage:0.1 μg/mouse; 1.0 μg/mouse; 5.0 μg/mouse
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Administration:single dose
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Result:Induced 100% mouse mortality within 30 hours and a blood bacterial count of 300-400 CFU/100 μl 12 hours post-injection at 5.0 μg/mouse.
Induced partial mouse mortality and a blood bacterial count of 50-100 CFU/100 μl 12 hours post-injection at 1.0 μg/mouse.
Caused no mortality when administered alone over 100 hours.
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Animal Model:BALB/c mice (6-8-week-old male; septicemia model via intraperitoneal challenge with clinical B. cereus JMU-06B-35)[3]
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Dosage:25 μg (a mixture of Sphingomyelin phosphodiesterase, Bacillus cereus, and complete Freund's adjuvant)
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Administration:s.c.; two doses at two-week intervals
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Result:Enabled 100% of mice to survive for 100 hours post-challenge, compared to 0% survival of sham-immunized mice within 30 hours.
Chemical Information
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CAS No. 9031-54-3
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Appearance Solid
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Color White to off-white
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SMILES
[Sphingomyelin phosphodiesterase, Bacillus cereus]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
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- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Nishiwaki H, et al. Purification and functional characterization of insecticidal sphingomyelinase C produced by Bacillus cereus. Eur J Biochem. 2004;271(3):601-606. [Content Brief]
[2]. Ikezawa H, et al. Effects of metal ions on sphingomyelinase activity of Bacillus cereus. Arch Biochem Biophys. 1986 Sep;249(2):588-95. [Content Brief]
[3]. Oda M, et al. Role of sphingomyelinase in infectious diseases caused by Bacillus cereus. PLoS One. 2012;7(6):e38054. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Sphingomyelin phosphodiesterase, Bacillus cereus Related Classifications
HY-P2936A Related Classifications
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