Taccalonolide A
Based on 1 Customer Validation
Taccalonolide A is a microtubule stabilizer, which is a steroid isolated from Tacca chantrieri, with cytotoxic and antimalarial activities. Taccalonolide A causes G2-M accumulation, Bcl-2 phosphorylation and initiation of apoptosis. Taccalonolide A is effective in vitro against cell lines that overexpress P-glycoprotein (Pgp) and multidrug resistance protein 7 (MRP7), with an IC50 of 622 nM for SK-OV-3 cells.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.29%
- CAS 番号: 108885-68-3
- 分子式: C36H46O14
- 分子量:702.74
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保管条件:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
IC50 & Target
microtubule[1]
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HeLa | IC50 |
5.3 μM
Compound: Taccalonolide A
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Antiproliferative activity against human HeLa cells by SRB assay
Antiproliferative activity against human HeLa cells by SRB assay
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[PMID: 24491636] |
| HeLa | IC50 |
5.32 μM
Compound: Taccalonolide A
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Antiproliferative activity against human HeLa cells after 48 hrs by SRB assay
Antiproliferative activity against human HeLa cells after 48 hrs by SRB assay
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[PMID: 23855953] |
| HeLa | IC50 |
5380 nM
Compound: 61
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Cytotoxicity against human HeLa cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay
Cytotoxicity against human HeLa cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay
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[PMID: 39068862] |
| HeLa | IC50 |
5400 nM
Compound: 1
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Antiproliferative activity against human HeLa cells after 48 hrs by sulforhodamine B assay
Antiproliferative activity against human HeLa cells after 48 hrs by sulforhodamine B assay
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[PMID: 29360362] |
| HeLa | IC50 |
594 nM
Compound: 1
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Antiproliferative activity against human HeLa cells after 48 hrs by SRB assay
Antiproliferative activity against human HeLa cells after 48 hrs by SRB assay
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[PMID: 21800839] |
化学情報
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CAS 番号 108885-68-3
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性状 Solid
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分子量 702.74
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分子式 C36H46O14
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Color White to off-white
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SMILES
C[C@@]12[C@]([C@@H](OC(C)=O)[C@]3([H])[C@]2([H])[C@@H](C=C(O4)[C@@]3([C@](O)(C4=O)C)C)C)([H])[C@@]([C@H]5O)([H])[C@]([C@]6([C@H]([C@@H](O7)[C@@H]7C[C@]6([H])C5=O)OC(C)=O)C)([H])[C@H](OC(C)=O)[C@@H]1OC(C)=O
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Structure Classification
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Initial Source
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶剤 & 溶解度
体外:
DMSO : 100 mg/mL (142.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.56 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.56 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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データシート (280 KB)
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SDS (252 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Tinley TL, et al. Taccalonolides E and A: Plant -derived steroids with microtubule-stabilizing activity. Cancer Res. 2003 Jun 15;63(12):3211-20. [Content Brief]
[2]. Risinger AL, et al. Taccalonolides: Novel microtubule stabilizers with clinical potential. Cancer Lett. 2010 May 1;291(1):14-9. [Content Brief]
[3]. Risinger AL, et al. The taccalonolides: microtubule stabilizers that circumvent clinically relevant taxane resistance mechanisms. Cancer Res. 2008 Nov 1;68(21):8881-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4230 mL | 7.1150 mL | 14.2300 mL | 35.5750 mL |
| 5 mM | 0.2846 mL | 1.4230 mL | 2.8460 mL | 7.1150 mL | |
| 10 mM | 0.1423 mL | 0.7115 mL | 1.4230 mL | 3.5575 mL | |
| 15 mM | 0.0949 mL | 0.4743 mL | 0.9487 mL | 2.3717 mL | |
| 20 mM | 0.0712 mL | 0.3558 mL | 0.7115 mL | 1.7788 mL | |
| 25 mM | 0.0569 mL | 0.2846 mL | 0.5692 mL | 1.4230 mL | |
| 30 mM | 0.0474 mL | 0.2372 mL | 0.4743 mL | 1.1858 mL | |
| 40 mM | 0.0356 mL | 0.1779 mL | 0.3558 mL | 0.8894 mL | |
| 50 mM | 0.0285 mL | 0.1423 mL | 0.2846 mL | 0.7115 mL | |
| 60 mM | 0.0237 mL | 0.1186 mL | 0.2372 mL | 0.5929 mL | |
| 80 mM | 0.0178 mL | 0.0889 mL | 0.1779 mL | 0.4447 mL | |
| 100 mM | 0.0142 mL | 0.0712 mL | 0.1423 mL | 0.3558 mL |
Taccalonolide A 関連分類
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